中文名稱(chēng)：氮雜二苯并環(huán)辛炔胺，二苯基環(huán)辛炔-氨基

中文別名：氨基丙酰氮雜二苯并環(huán)辛炔

英文名稱(chēng)：Azadibenzocylooctyne-amine

英文別名：Dibenzocyclooctyne-amine;DBCO-NH2;DBCO-amine;ADIBO-AMINE;

CAS號(hào)：1255942-06-3

分子式：C18H16N2O

分子量：276.33200

質(zhì)量：276.12600

PSA：46.33000

LogP：3.04710

結(jié)構(gòu)式：

名稱(chēng)：DBCO-Amine   MW:276.33   CAS:1255942-06-3

英文簡(jiǎn)介：

A carboxyl-reactive building block containing DBCO moiety and a terminal primary amine group.

DBCO-Amine is a simple DBCO-containing reagent possessing a terminal primary amines group (–NH2) that reacts with carboxyl groups in the presence of activators (e.g. EDC, or DCC) or with activated esters (e.g. NHS esters) forming stable amide bonds. A short spacer arm adds minimal mass to modified molecules (273.3 daltons).

 

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名稱(chēng)：DBCO-PEG4-Amine   MW:523.62

英文簡(jiǎn)介：

A carbonyl-reactive building block with enhanced solubility in aqueous media.

DBCO-PEG4-Amine is a polyethylene glycol-containing reagent with terminal primary amines (–NH2) that reacts with carboxyl groups in the presence of activators (e.g. EDC, or DCC) or with activated esters (e.g. NHS esters) forming stable amide bonds. A hydrophilic spacer arm enhances solubility in aqueous buffers.

 

名稱(chēng)：DBCO-NHS Ester   MW：402.40

英文介紹：

DBCO-NHS Ester reacts with primary amines

This product is not recommended for labeling of proteins or any other biopolymers in aqueous buffers due to poor aqueous solubility.

 

名稱(chēng)：Photocleavable DBCO-NHS ester  MW:945.96

英文介紹：

An amine-reactive, photocleavable labeling reagent.

Photocleavable DBCO-NHS ester reagent contains an amine reactive group linked to DBCO moiety through a spacer arm containing a photocleavable moiety. Molecules jointed by the copper-free click reaction can be efficiently photoreleased, typically >90% in 5-25 minutes using an inexpensive, near-UV, low intensity lamp (e.g. 365 nm lamp at 1-5 mW/cm2).

 

名稱(chēng): DBCO-C6-NHS Ester   MW:430.45

英文介紹：

An activated ester with extended 6-carbon atom long spacer.

DBCO-NHS Ester reacts with primary amines (e.g., side chain of lysine residues or aminosilane-coated surfaces) at pH 7-9 to form covalent bonds. Extended 6-carbon spacer arm is beneficial for some application. This reagent has limited shelf life; however, corresponding acid and its derivatives are found to be stable over long period of time. DBCO-Sulfo-NHS-Ester, a water-soluble version of this product is also available for labeling experiments where the presence of organic solvents is highly undesirable.

 

名稱(chēng)：DBCO-PEG5-NHS Ester  MW:693.74

英文簡(jiǎn)介：

An amine-reactive labeling reagent with enhanced water stability and solubility.

DBCO-PEG5-NHS Ester reacts specifically and efficiently with a primary amine (e.g., side chain of lysine residues or aminosilane-coated surfaces) at pH 7-9 to form a covalent bond. The hydrophilic polyethylene glycol (PEG) spacer arm imparts water solubility that is transferred to the labeled molecule, thus reducing aggregation of labeled proteins stored in solution. The PEG spacer arm provides a long and flexible connection that minimizes steric hindrance involved with ligation to complementary azide-containing molecules.

 

名稱(chēng)：DBCO-PEG4-NHS Ester  MW:649.68

英文簡(jiǎn)介：

An amine-reactive labeling reagent with enhanced water solubility provided by a PEG4 spacer.

DBCO-PEG4-NHS Ester reacts specifically and efficiently with a primary amine (e.g., side chain of lysine residues or aminosilane-coated surfaces) at pH 7-9 to form a covalent bond. The hydrophilic polyethylene glycol (PEG) spacer arm imparts water solubility that is transferred to the labeled molecule, thus reducing aggregation of labeled proteins stored in solution. The PEG spacer arm provides a long and flexible connection that minimizes steric hindrance involved with ligation to complementary azide-containing molecules.

 

名稱(chēng)：DBCO-Sulfo-NHS Ester  MW:532.50

英文簡(jiǎn)介：

A water-soluble reagent for labeling antibodies, proteins and other molecules containing primary amines.

DBCO-Sulfo-NHS-Ester enables simple and efficient labeling of antibodies, proteins and any other primary amine-containing macromolecules with DBCO moiety. This reagent is water-soluble, enabling labeling to be performed in the absence of organic solvents such as DMSO or DMF for applications that cannot tolerate solvents or are complicated by their inclusion. Specific labeling of cell surface proteins is another common application for these uniquely water-soluble and membrane impermeable reagents.

 

名稱(chēng)：DBCO-Acid   MW:305.11

英文簡(jiǎn)介：

DBCO-Acid is an amine reactive, non-activated cyclooctyne building block.

The use of strained cyclooctynes instead of terminal alkynes allows for overcoming a major drawback of copper-catalyzed azide-alkyne cycloaddition (CuAAC) - the need for the toxic copper catalyst. The ring strain in cyclooctynes, such as DBCO-Acid makes them highly reactive in the cycloaddition reaction. This reaction was termed as the strain-promoted alkyne-azide cycloaddition (SPAAC) or copper-free click reaction and has been widely used for bioconjugation and for labeling living cells and living animals.

 

名稱(chēng)：DBCO-C6-Acid   MW:333.83

英文簡(jiǎn)介：

An analog of DBCO-Acid with improved stability and solubility in commonly used organic solvents.

DBCO reagents, also known as ADIBO or DIBAC are routinely used building blocks for synthesis of complex molecules to be used in Cu-free ligation applications.

Using DBCO-C6-Acid primary or secondary amines can be easily functionalized with DBCO moiety in the presence of activators (e.g. EDC, or HATU) through a stable amide bond. A longer carbon spacer arm improves stability and solubility of this reagent in commonly used organic solvents.

 

名稱(chēng)：DBCO-PEG5-Acid    MW:596.67

英文簡(jiǎn)介：

An amine-reactive building block with enhanced stability and solubility in aqueous media.

DBCO-PEG5-Acid is a polyethylene glycol-containing reagent with terminal carboxylic acid (–COOH) that can be reacted with primary or secondary amine groups in the presence activators (e.g. EDC) forming a stable amide bond. The PEG spacer arm is hydrophilic and provides greater solubility to labeled molecules compared to reagents having only hydrocarbon spacers. The PEG spacer arm also gives the reagent a long and flexible connection to minimize steric hindrance involved with reacting with complementary azido-modified molecules.

 

名稱(chēng)：DBCO-PEG4-Acid  MW:552.62

英文簡(jiǎn)介：

An amine-reactive building block with enhanced solubility in aqueous media.

DBCO-PEG4-Acid is a polyethylene glycol-containing reagent with terminal carboxylic acid (–COOH) that can be reacted with primary or secondary amine groups in the presence activators (e.g. EDC) forming a stable amide bond. The PEG spacer arm is hydrophilic and provides greater solubility to labeled molecules compared to reagents having only hydrocarbon spacers

 

名稱(chēng)：DBCO-PEG4-4-Formylbenzoate    MW:655.77

英文簡(jiǎn)介：

A heterobifunctional reagent for labeling aminooxy-containing compounds.

DBCO-PEG4-4-Formylbenzoate reacts specifically and efficiently with an aminooxy group at pH 4-5 to form a covalent oxime bond. The oxime ligation with aromatic aldehydes is highly chemoselective and is compatible with other functional groups present in peptides or biomolecules. An aniline catalysis can be used to activate aromatic aldehyde towards an aminooxy group and greatly enhance the rate of ligation reaction.

 

名稱(chēng)：ThioLinker-DBCO

英文簡(jiǎn)介：

A unique, water-soluble, bis-alkylating reagent that allows for site-specific labeling of antibodies under mild aqueous conditions.

The traditional sulfhydryl labeling chemistry cleaves disulfide bridges and leaves them in “open form”, which may compromise the stability and function of an antibody or other proteins. ThioLinkerTM-DCBO re-bridges the disulfide bond, and enhances the structural stability of the protein. In addition, conjugates prepared with ThioLinkerTM reagents benefits from reduced heterogeneity and better stability.

 

名稱(chēng)：DBCO-Maleimide  MW:427.75

英文簡(jiǎn)介：

A short-length, sulfhydryl-reactive labeling reagent that contains a short hydrocarbon spacer.

DBCO-Maleimide enables simple and efficient incorporation of DBCO moiety onto cysteine-containing peptides or other thiol-containing molecules. The maleimide group specifically and efficiently reacts with reduced thiols (sulfhydryl groups, –SH) at pH 6.5 to 7.5 to form stable thioether bonds. A short spacer arm adds minimal mass to modified molecules (427.4 daltons).

 

名稱(chēng)：DBCO-PEG4-Maleimide  MW:674.74

英文簡(jiǎn)介：

A mid-length, sulfhydryl-reactive labeling reagent with enhanced water solubility provided by a PEG4 spacer.

DBCO-PEG4-Maleimide enables simple and efficient incorporation of DBCO moiety onto antibodies, cysteine-containing peptides and other thiol-containing molecules. The hydrophilic polyethylene glycol (PEG) spacer arm imparts water solubility that is transferred to the labeled molecule, thus reducing aggregation of labeled proteins stored in the solution. The PEG spacer arm provides a long and flexible connection that minimizes steric hindrance involved with ligation to complementary azide-containing molecules.

 

名稱(chēng)：Bis-sulfone-PEG4-DBCO   MW：1006.19

英文簡(jiǎn)介：

A bis-alkylating labeling reagent used to create conjugates with enhanced structural stability.

Bis-sulfone-PEG4-DBCO enables site-specific incorporation of DBCO moiety onto antibodies to form well defined conjugates. Unlike conjugates prepared through conventional, maleimide-based labeling chemistry that leaves disulfide bridges in “open form” and produces a heterogeneous mixture labeled species with DAR 1-8, conjugates prepared with bis-sulfone reagents benefits from reduced heterogeneity and enhanced stability.

 

名稱(chēng)：Bis-sulfone-PEG3-Azide  MW:700.82

英文簡(jiǎn)介：

A bis-alkylating labeling reagent used to create well-defined conjugates with enhanced structural stability.

Bis-sulfone-PEG3-Azide enables site-specific incorporation of an azide group onto antibodies to form well defined conjugates. Unlike conjugates prepared through conventional, maleimide-based labeling chemistry that leaves disulfide bridges in “open form” and produces a heterogeneous mixture labeled species with DAR 1-8, conjugates prepared with bis-sulfone reagents benefits from reduced heterogeneity and enhanced stability.

 

DBCO Reagents-Bifunctional Crosslinkers產(chǎn)品詳細(xì)英文介紹和使用說(shuō)明：

名稱(chēng)：DBCO-PEG4-DBCO   MW:854.92

英文簡(jiǎn)介：

An azide-to-azide homobifunctional cross-linker with a hydrophilic PEG4 spacer arm.

DBCO-PEG4-DBCO reacts specifically and efficiently with an azide group (e.g., a side chain of L-azidohomoalanine) to form a stable triazole moiety.

 

名稱(chēng)：TCO-PEG12-DBCO

英文簡(jiǎn)介：

An azide-to-tetrazine heterobifunctional cross-linker with a hydrophilic PEG12 spacer arm.

Heterobifunctional reagents are used for converting azido-containing biomolecules into TCO-modified biomolecules. TCO-PEG12-DBCO can serve as a heterobifunctional azide-to-tetrazine cross-linker for covalently linking azido- and tetrazine-modified peptides or biopolymers. The PEG12 spacer arm provides a long and flexible connection that minimizes steric hindrance involved with ligation to complementary reagent.

 

名稱(chēng)：Methyltetrazine-DBCO

英文簡(jiǎn)介：

A water-soluble azide-to-TCO heterobifunctional crosslinker.

Heterobifunctional reagents used for converting azido-containing peptides or protein into tetrazine-modified peptides or protein without the need for catalyst or axillary reagents. Tetrazine-DBCO can serve as a heterobifunctional azide-to-TCO cross-linker for covalently linking azido- and TCO-modified peptides or biopolymers.

 

名稱(chēng)：Tetrazine-PEG12-DBCO

英文簡(jiǎn)介：

An azide-to-TCO heterobifunctional crosslinker with a hydrophilic PEG12 spacer arm.

Heterobifunctional reagents used for converting azido-containing peptides or protein into tetrazine-modified peptides or protein without the need for catalyst or axillary reagents. Tetrazine-PEG12-DBCO can serve as a heterobifunctional azide-to-TCO cross-linker for covalently linking azido- and TCO-modified peptides or biopolymers.

 

名稱(chēng)：DBCO-PEG4-4-Formylbenzoate

英文簡(jiǎn)介：

A heterobifunctional reagent for labeling aminooxy-containing compounds.

DBCO-PEG4-4-Formylbenzoate reacts specifically and efficiently with an aminooxy group at pH 4-5 to form a covalent oxime bond. The oxime ligation with aromatic aldehydes is highly chemoselective and is compatible with other functional groups present in peptides or biomolecules. An aniline catalysis can be used to activate aromatic aldehyde towards an aminooxy group and greatly enhance the rate of ligation reaction.

 

名稱(chēng)：DBCO-PEG4-Biotin

英文簡(jiǎn)介：

An azide-reactive biotinylation reagent with an extended PEG4 spacer arm.

DBCO reacts with an azide to produce a stable triazole, also referred to as the Cu(I)‐free or strain-promoted click reaction. The azide group, which is the reactive target of DBCO-activated biotin, can be introduced into proteins or other cellular targets via azide labeling reagents or through in vivo labeling with azide-derivatives of naturally occurring metabolic building blocks. This reaction possesses extreme selectivity and biocompatibility, such that the complimentary reagents can form covalent bonds within richly functionalized biological systems, and, in some cases, living organisms.

 

名稱(chēng)：WS DBCO-Biotin

英文簡(jiǎn)介：

A water-soluble, azide-reactive biotinylation reagent.

DBCO reacts with an azide to produce a stable triazole, also referred to as the Cu(I)‐free or strain-promoted click reaction. The azide group, which is the reactive target of DBCO-activated biotin, can be introduced into proteins or other cellular targets via azide labeling reagents or through in vivo labeling with azide-derivatives of naturally occurring metabolic building blocks. This reaction is extremely selective and biocompatible, such that the complimentary reagents can form a covalent bond within richly functionalized biological systems, and, in some cases, living organisms.

 

名稱(chēng)：photoclevable DBCO-Biotin

英文簡(jiǎn)介：

An azide-reactive, photoclevable biotin probe that allows for reagent-free release of the captured biomolecules from streptavidin.Extraordinary strength of the streptavidin-biotin interaction allows for efficient capturing of even highly dilute targets; however, it makes recovery of proteins from affinity resins challenging.

 

名稱(chēng)：DBCO-PEG4-Desthiobiotin

英文簡(jiǎn)介：

An azide-reactive desthiobiotin reagent that allows easy elution from streptavidin.

Desthiobiotin is a single-ring, sulfur-free analog of biotin that binds to streptavidin with nearly equal specificity but less affinity than biotin (Kd = 1011 vs. 1015M, respectively). Consequently, desthiobiotinylated proteins can be eluted readily and specifically from streptavidin affinity resin using mild conditions based on competitive displacement with free biotin. For pull-down experiments with biological samples, this soft-release characteristic of desthiobiotin also helps to minimize co-purification of endogenous biotinylated molecules, which remain bound to streptavidin upon elution of the target protein complexes with free biotin.

 

名稱(chēng)：WS-PC DBCO-Biotin

英文簡(jiǎn)介：

A water-soluble, photoclevable biotin probe that allows for reagent-free release of the captured biomolecules from streptavidin.

Extraordinary strength of the streptavidin-biotin interaction allows for efficient capturing of even highly dilute targets; however, it makes recovery of proteins from affinity resins challenging. Photocleavable biotin probes eliminate a major limitation of the streptavidin-biotin affinity purification. This reagent contains a biotin moiety linked to an azide moiety through a spacer arm containing a photocleavable moiety. Captured biomolecules can be efficiently photoreleased, typically >90% in 5-25 minutes using an inexpensive, near-UV, low intensity lamp (e.g. 365 nm lamp at 1-5 mW/cm2).

 

名稱(chēng)：Dde Biotin-DBCO

英文簡(jiǎn)介：

A hydrazine cleavable biotinylation reagent for selective enrichment of azide-tagged biopolymers via SPAAC reaction.

Extraordinary strength of the streptavidin-biotin interaction allows for efficient capturing of even highly dilute targets; however, it makes recovery of proteins from affinity resins challenging. Dde Biotin-DBCO probe eliminates a major limitation of the streptavidin-biotin affinity purification. This reagent contains a biotin moiety linked to an alkyne moiety through a spacer arm containing a Dde linker. Captured biomolecules can be efficiently released, typically >90%.

 

名稱(chēng)：Diazo Biotin-DBCO

英文簡(jiǎn)介：

A sodium dithionite (Na2S2O4) cleavable biotinylation reagent for selective enrichment of azide-tagged biopolymers via SPAAC reaction.

Diazo Biotin-DBCO allows capture of azide-tagged biomolecules via the Cu-free click reaction under mild aqueous buffer conditions. This reaction possesses extreme biocompatibility, such that the complementary reagents can form covalent bonds within living organisms. Biotinylated protein targets can be selectively captured through conventional affinity purification protocols that exploit the biotin - streptavidin interaction. Captured protein targets can be released under mild conditions with 50 mM sodium dithionite solution, and identified by mass spectrometry, ELISA, dot blot, or Western blot applications.

 

名稱(chēng)：DBCO-S-S-PEG3-Biotin

英文簡(jiǎn)介：

A cleavable biotinylation reagent for specific enrichment azide-tagged biomolecules.

DBCO-S-S-PEG3-Biotin enables simple and efficient biotin labeling of azide-tagged antibodies, proteins and other macromolecules. The DBCO group reacts with an azide to produce an stable triazole, which is also referred to as the Cu(I)-free or strain-promoted click reaction. The hydrophilic PEG spacer arm imparts water solubility and reduces aggregation of labeled proteins stored in the solution. The integral disulfide bond enables the biotin group to be cleaved from the labeled molecule using DTT or other reducing agents.

 

名稱(chēng)：Carboxyrhodamine 110 DBCO

英文簡(jiǎn)介：

A green fluorescence-emitting probe with excitation/emission maximum of 501/523 nm

DBCO-activated Carboxyrhodamine110 dye reacts with azides to produce a stable triazole, also referred to as the Cu-free click reaction, and does not require Cu-catalyst or elevated temperatures. This reaction is extremely selective and biocompatible, such that the complimentary reagents can form a covalent bond within richly functionalized biological systems, and, in some cases, living organisms.

 

名稱(chēng)：Sulforhodamine B DBCO

英文簡(jiǎn)介：

A red fluorescence-emitting probe with excitation/emission maximum of 568/585 nm

DBCO-activated Sulforhodamine B dye reacts with azides to produce a stable triazole, also referred to as the Cu-free click reaction, and does not require a Cu-catalyst or elevated temperatures. This reaction possesses extreme selectivity and biocompatibility, such that the complimentary reagents can form covalent bonds within richly functionalized biological systems, and, in some cases, living organisms.

 

名稱(chēng)：TAMRA DBCO

英文簡(jiǎn)介：

An orange-to-red fluorescence-emitting probe with excitation/emission maximum of 554/567 nm

DBCO-activated TAMRA dye reacts with azides to produce a stable triazole, also referred to as the Cu-free click reaction, and does not require Cu-catalyst or elevated temperatures. This reaction possesses extreme selectivity and biocompatibility, such that the complimentary reagents can form covalent bonds within richly functionalized biological systems, and, in some cases, living organisms.

 

名稱(chēng)：Sulforhodamine 101 DBCO

英文簡(jiǎn)介：

A red fluorescence-emitting probe with excitation/emission maximum of 586/601 nm

DBCO-activated Sulforhodamine 101 dye reacts with azides to produce a stable triazole, also referred to as the Cu-free click reaction, and does not require a Cu-catalyst or elevated temperatures. This reaction is extremely selective and biocompatible, such that the complimentary reagents can form covalent bonds within richly functionalized biological systems, and, in some cases, living organisms.

 

名稱(chēng)：Cy3 DBCO

英文簡(jiǎn)介：

An azide-reactive Cy3 dye for chemoselective labeling and detection of azido-tagged molecules via the Cu-free click reaction.

 

名稱(chēng)：Cy5 DBCO

英文簡(jiǎn)介：

A water-soluble, azide-reactive Cy5 dye for chemoselective labeling and detection of azido-tagged molecules via the Cu-free click reaction.

 

名稱(chēng)：Cy5.5 DBCO

英文簡(jiǎn)介：

An azide-reactive, water soluble Cy5.5 dye for chemoselective labeling and detection of azido-tagged molecules.

 

名稱(chēng)：Cy7 DBCO

英文簡(jiǎn)介：

An azide-reactive, water-soluble Cy7 dye for chemoselective labeling and detection of azido-tagged molecules via the Cu-free click reaction.

 

名稱(chēng)：DBCO-PEG4-Phosphoramidite

英文簡(jiǎn)介：

DBCO-PEG4-Phosphoramidite is a reagent for incorporation of DBCO moiety onto an oligonucleotide for subsequent copper-free click elaboration.

 

名稱(chēng)：DBCO-PEG4-Hydroxyl

英文簡(jiǎn)介：

A modification reagent containing a long 4-unit polyethylene glycol (PEG4) spacer arm and a terminal primary hydroxyl group.

 

TCO Reagents-Amine Reactive點(diǎn)擊化學(xué)詳細(xì)介紹：

名稱(chēng)：TCO-NHS Ester

英文簡(jiǎn)介

An amine-reactive, succinimidyl carbonate/NHS functionalized cyclooctene building block.

NHS functionalized cyclooctene derivative is a useful building block for incorporation of the cyclooctene moiety into amine containing compounds or biomolecules. A TCO functionalized compounds will react with tetrazine containing compounds or biomolecules without pre-activation or need for a catalyst to result in a stable covalent linkage. TCO-NHS ester is supplied as a single axial (major) diastereomer. Specially formulated crystalline provides easy handling and extended shelf life.

 

名稱(chēng)：TCO-PEG4-NHS Ester

英文簡(jiǎn)介

An amine-reactive labeling reagents with enhanced solubility in aqueous buffers provided by a PEG4 spacer.

TCO-PEG4-NHS Ester reacts specifically and efficiently with a primary amine (e.g., side chain of lysine residues or aminosilane-coated surfaces) at pH 7-9 to form a covalent bond. The hydrophilic polyethylene glycol (PEG) spacer arm significantly improves labeling efficiency, imparts water solubility, and reduces aggregation of labeled proteins stored in solution. The PEG spacer arm also gives the reagent a long and flexible connection that minimizes steric hindrance involved with ligation to complementary tetrazine-containing molecules.

 

名稱(chēng)：TCO-PEG12-NHS Ester

英文簡(jiǎn)介：

An amine-reactive labeling reagents with extended PEG12 spacer arm.

TCO-PEG12-NHS Ester reacts specifically and efficiently with a primary amine (e.g., side chain of lysine residues or aminosilane-coated surfaces) at pH 7-9 to form a covalent bond. The hydrophilic polyethylene glycol (PEG) spacer arm significantly improves labeling efficiency, imparts water solubility, and reduces aggregation of labeled proteins stored in solution. The PEG spacer arm also gives the reagent a long and flexible connection that minimizes steric hindrance involved with ligation to complementary tetrazine-containing molecules.

 

名稱(chēng)：UV-Tracer TCO-NHS Ester

英文簡(jiǎn)介：

UV-TracerTM TCO-NHS Ester is the ultimate labeling reagent where great precision in optimization and characterization of crosslinking process is required.

UV-TracerTM TCO‐NHS Ester is an amine‐reactive labeling reagent with built‐in signal quantification capability. It was carefully engineered to provide researchers with maximum control and reproducibility over the labeling process while preserving maximum reactivity toward complementary tetrazine groups.

 

名稱(chēng)：TCO-PEG4-Acid

英文簡(jiǎn)介：

An amine-reactive, non-activated building block with enhanced solubility in aqueous buffers.

TCO-PEG4-Acid is a polyethylene glycol-containing reagent with terminal carboxylic acid (–COOH) that can be reacted with primary or secondary amine groups in the presence of activators (e.g. EDC) forming a stable amide bond. The PEG spacer arm is hydrophilic and provides greater solubility to labeled molecules compared to reagents having only hydrocarbon spacers. The PEG spacer arm also gives the reagent a long and flexible connection to minimize steric hindrance involved with reacting with complementary tetrazine-modified molecules.

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