ICG maleimide，吲哚菁綠-馬來酰亞胺

ICG maleimide，吲哚菁綠-馬來酰亞胺 我公司是國內(nèi)生命科學(xué)和生物技術(shù)領(lǐng)域試劑供應(yīng)商，從成立初期就致力于為生命科學(xué)和生物技術(shù)領(lǐng)域提供優(yōu)質(zhì)產(chǎn)品和技術(shù)服務(wù)，力求將的產(chǎn)品和服務(wù)奉獻(xiàn)給我們的客戶。

ICG maleimide，吲哚菁綠-馬來酰亞胺

英文名：ICG maleimide，ICG-MAL

中文名：吲哚菁綠-馬來酰亞胺

外觀：綠色粉末

溶解度：二氯甲烷

純度：95%+

結(jié)構(gòu)式：

ICG maleimide，吲哚菁綠-馬來酰亞胺

ICG is a tricarbocyanine-type dye with NIR-absorbing properties (peak absorption around 800 nm) and emission maximum at 807 nm (10% v/vDMSO/PBS). Little absorption in the visible range accounts for the low autofluorescence, tissue absorbance, and scattering at NIR wavelengths (700-900 nm).

The maleimide of the ICG dye offers the opportunity to develop optimal conjugates. Maleimide active group provides an efficient and convenient way to selectively link ICG dye to sulfhydryl groups (free thiol, R-SH) on various substrates (antibodies[1], peptides, proteins, oligonucleotides, small molecule drugs etc.) at neutral (physiological) pH without any activation. Maleimides have very low reactivity with amines, alcohols, and phenols (incl. tyrosine and histidine) and do not react with histidine and methionine thus providing very high labeling selectivity.

It has been reported that conjugation to some antibodies may cause decrease in fluorescence intensity of ICG bound to antibody due to the formation of H-dimers4. However, fluorescence is restored after cell binding and internalization, which can be used to design an activatable NIR fluorescent probe. Fluorescence intensity of the unbound ICG-antibody conjugate can be dramatically increased (up to 50-fold) by addition of SDS and β-mercaptoethanol, which diminishes π- π stacking5.

近紅外熒光染料Near IRDyes激發(fā)和發(fā)射波長和顏色圖：

ICG NHS ester的NHS可以和蛋白、多肽或抗體上面的NH2發(fā)生反應(yīng)鏈接到一起。

ICG-maleimide的馬來酰亞胺可以和蛋白、多肽、小分子或抗體上的SH巰基反應(yīng)連接。

ICG-Amine的NH2可以和蛋白、多肽小分子上面的NHS反應(yīng)連接起來。

ICG和PEG鏈接可在體內(nèi)長循環(huán)。

  
ICG衍生物產(chǎn)品：
ICG修飾蛋白類產(chǎn)品
ICG-Avidin
ICG-Streptavidin
ICG-BSA      
ICG-Dextran
ICG-Alginate
ICG-Chitosan
ICG-Hyaluronic acid
ICG-Heparin
ICG-Ficoll
ICG-Cellulose
ICG-Concanavalin A
ICG-Transferrin
ICG-Polylysine
ICG NHS ester
ICG Vinylsulfone
ICG isothiocyanate
ICG Carboxlaic acid
ICG Maleimide
ICG amine
ICG thiol
ICG dichlorotriazine
ICG Hydrazide
ICG alkyne
ICG Avidin
ICG Biotin

吲哚菁綠直接標(biāo)記在磷脂上:
DSPE-ICG
DPPE-ICG
DMPE-ICG
DLPE-ICG
DOPE-ICG
 
吲哚菁綠通過一個(gè)小分子PEG偶連到磷脂上,PEG重復(fù)單元從2-5個(gè)不等:
DSPE-PEG2-ICG
DSPE-PEG3-ICG
DSPE-PEG4-ICG
 
吲哚菁綠連接高分子PEG再連接到磷脂上，PEG分子量有550/750/1000/2000/3400/5000
ICG-PEG-DSPE
ICG-PEG-DMPE
ICG-PEG-DPPE
ICG-PEG-DLPE
ICG-PEG-DOPE
 
吲哚菁綠標(biāo)記PEG末端帶活性基團(tuán)產(chǎn)品,PEG分子量1000/2000/3400/5000 Da ：
mPEG-ICG
ICG-PEG-NH2
ICG-PEG-COOH
ICG-PEG-MAL
ICG-PEG-NHS
ICG-PEG-N3
ICG-PEG-Biotin
ICG-PEG-SH
ICG-PEG-OH
ICG-PEG-Alkyne
ICG-PEG-CHO
ICG-PEG-Folate
ICG-PEG-cRGD
 
吲哚菁綠修飾一些聚合物產(chǎn)品：
PLGA-ICG
PCL-ICG
PDLLA-ICG
Dextran-ICG
PEI-ICG
PAMAM-ICG
PLL-ICG
 
吲哚菁綠修飾一些共聚物產(chǎn)品：
PLGA-PEG-ICG
PCL-PEG-ICG
PLA-PEG-ICG
mPEG-PLGA-ICG
mPEG-PLA-ICG
mPEG-PCL-ICG
PLGA-PEI-ICG
Dextran-PLGA-ICG
Dextran-PCL-ICG
ICG-PEG-PEI