武漢原生原代生物醫(yī)藥科技有限公司

PriCells: Isolation of human atrial or ventricular cells

時(shí)間:2022-1-4 閱讀:255
分享:

PriCells: Isolation of human atrial or ventricular cells                                          


1. Human tissue was derived from atrial or ventricular biopsy specimens belonging to patients undergoing heart surgery.
2. Isolated myocardial tissue was cut into 1- to 2-mm3 pieces.
3. Isolated myocardial tissue was washed with Ca2+ -Mg2+free phosphate-buffered solution (PBS).
4. Isolated myocardial tissue was digested three times for 5 minutes at 37℃ with 0.2% trypsin and 0.1% collagenase IV.
5. The obtained cells were discarded, and the remaining tissue fragments washed with primary cell culture medium and primary cell culture supplemented with 10% fetal calf serum, 100 U/mL penicillin G, 100 µg/mL streptomycin, and 2 mmol/L L-glutamine were cultured at 37℃and 5% CO2.
6. Twenty-four hours later, the medium was again replaced with primary cell medium and supplement.


會(huì)員登錄

×

請(qǐng)輸入賬號(hào)

請(qǐng)輸入密碼

=

請(qǐng)輸驗(yàn)證碼

收藏該商鋪

X
該信息已收藏!
標(biāo)簽:
保存成功

(空格分隔,最多3個(gè),單個(gè)標(biāo)簽最多10個(gè)字符)

常用:

提示

X
您的留言已提交成功!我們將在第一時(shí)間回復(fù)您~
撥打電話 產(chǎn)品分類(lèi)
在線留言