PRDM9 Chemiluminescent Assay Kit 返回列表頁

The PRDM9 Chemiluminescent Assay Kit is designed to measure PRDM9 activity for screening and profiling applications, using purified PRDM9. The PRDM9 Chemiluminescent Assay Kit comes in a convenient format, with 8-well strips pre-coated with histone H3 peptide substrate, an antibody against methylated lysine on Histone H3, a secondary HRP-labeled antibody, S-adenosylmethionine, methyltransferase assay buffer, and enough purified PRDM9 enzyme for 100 enzyme reactions. The key to the PRDM9 Chemiluminescent Assay Kit is a highly specific antibody that recognizes methylated K36 residue of Histone H3. With this kit, only three simple steps on a microtiter plate are required for methyltransferase detection. First, S-adenosylmethionine is incubated with a sample containing assay buffer and methyltransferase enzyme. Next, primary antibody is added. Finally, the plates are treated with an HRP-labeled secondary antibody followed by the addition of the HRP substrate to produce chemiluminescence that can be measured using a chemiluminescence reader.