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細(xì)胞毒性T淋巴細(xì)胞相關(guān)抗原4ELISA試劑盒

時(shí)間:2018/8/16閱讀:440
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細(xì)胞毒性T淋巴細(xì)胞相關(guān)抗原4ELISA試劑盒 elisa試劑盒檢測(cè)應(yīng)用

細(xì)胞毒性T淋巴細(xì)胞相關(guān)抗原4ELISA試劑盒的產(chǎn)品介紹

細(xì)胞毒性T淋巴細(xì)胞相關(guān)抗原4ELISA試劑盒由我司*銷售細(xì)胞毒性T淋巴細(xì)胞相關(guān)抗原4ELISA試劑盒。

細(xì)胞毒性T淋巴細(xì)胞相關(guān)抗原4ELISA試劑盒

細(xì)胞毒性T淋巴細(xì)胞相關(guān)抗原4ELISA試劑盒

細(xì)胞毒性T淋巴細(xì)胞相關(guān)抗原4ELISA試劑盒

標(biāo)準(zhǔn)品的稀釋原則: 2 瓶,每瓶臨用前以樣品稀釋液稀釋至 1ml ,蓋好后靜置 10 分鐘以上,然后反復(fù)顛倒 / 搓動(dòng)以助溶解,其濃度為 300 pg/ml ,做系列倍比稀釋后,分別稀釋 300 pg/ml , 150 pg/ml , 75 pg/ml , 37.5 pg/ml , 18.5 pg/ml , 9 pg/ml , 4.5 pg/ml ,樣品稀釋液直接作為標(biāo)準(zhǔn)濃度 0 pg/ml ,臨用前 15 分鐘內(nèi)配制。 如配制 150 pg/ml 標(biāo)準(zhǔn)品:取 0.5ml (不要少于 0.5ml ) 300 pg/ml 的上述標(biāo)準(zhǔn)品加入含 0.5ml 樣品稀釋液的 Eppendorf 管中,混勻即可,其余濃度以此類推。 生物素標(biāo)記抗體的稀釋原則: 臨用前以生物素標(biāo)記抗體稀釋液稀釋,稀釋前根據(jù)預(yù)先計(jì)算好的每次實(shí)驗(yàn)所需的總量配制(每孔 100 μ l ),實(shí)際配制時(shí)應(yīng)多配制 0.1-0.2ml 。如 10 μ l 生物素標(biāo)記抗體加 990 μ l 生物素標(biāo)記抗體稀釋液的比例配制,輕輕混勻,在使用前一小時(shí)內(nèi)配制。 辣根過(guò)氧化物酶標(biāo)記親和素的稀釋原則: 臨用前以辣根過(guò)氧化物酶標(biāo)記親和素稀釋液稀釋,稀釋前根據(jù)預(yù)先計(jì)算好的每次實(shí)驗(yàn)所需的總量配制(每孔 100 μ l ),實(shí)際配制時(shí)應(yīng)多配制 0.1-0.2ml 。如 10 μ l 辣根過(guò)氧化物酶標(biāo)記親和素加 990 μ l 辣根過(guò)氧化物酶標(biāo)記親和素稀釋液 的比例配制,輕輕混勻,在使用前一小時(shí)內(nèi)配制。

細(xì)胞毒性T淋巴細(xì)胞相關(guān)抗原4ELISA試劑盒

感謝各位對(duì)本公司的支持與認(rèn)可,我們將再接再厲為您們提供的產(chǎn)品與服務(wù)。細(xì)胞毒性T淋巴細(xì)胞相關(guān)抗原4ELISA試劑盒外,還有更多相關(guān)實(shí)驗(yàn)產(chǎn)品,標(biāo)準(zhǔn)品,細(xì)胞株和實(shí)驗(yàn)技術(shù)服務(wù)等等前期后續(xù)服務(wù)。如果您對(duì)考慮購(gòu)買的ELISA試劑盒還有任何疑問(wèn),都可以直接向我們咨詢。為您服務(wù)是我們的榮幸。

(編輯:tanxi)

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