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超氧化物岐化酶ELISA試劑盒使用說明

時間：2018/9/3閱讀：445
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超氧化物岐化酶ELISA試劑盒 elisa試劑盒檢測應(yīng)用

超氧化物岐化酶ELISA試劑盒的產(chǎn)品介紹

超氧化物岐化酶ELISA試劑盒由我司*銷售超氧化物岐化酶ELISA試劑盒。

超氧化物岐化酶ELISA試劑盒 (多種種屬)實驗科研認可品牌

超氧化物岐化酶ELISA試劑盒

超氧化物岐化酶ELISA試劑盒

標準品的稀釋原則： 2 瓶，每瓶臨用前以樣品稀釋液稀釋至 1ml ，蓋好后靜置 10 分鐘以上，然后反復(fù)顛倒 / 搓動以助溶解，其濃度為 300 pg/ml ，做系列倍比稀釋后，分別稀釋 300 pg/ml ， 150 pg/ml ， 75 pg/ml ， 37.5 pg/ml ， 18.5 pg/ml ， 9 pg/ml ， 4.5 pg/ml ，樣品稀釋液直接作為標準濃度 0 pg/ml ，臨用前 15 分鐘內(nèi)配制。 如配制 150 pg/ml 標準品：取 0.5ml （不要少于 0.5ml ） 300 pg/ml 的上述標準品加入含 0.5ml 樣品稀釋液的 Eppendorf 管中，混勻即可，其余濃度以此類推。 生物素標記抗體的稀釋原則： 臨用前以生物素標記抗體稀釋液稀釋，稀釋前根據(jù)預(yù)先計算好的每次實驗所需的總量配制（每孔 100 μ l ），實際配制時應(yīng)多配制 0.1-0.2ml 。如 10 μ l 生物素標記抗體加 990 μ l 生物素標記抗體稀釋液的比例配制，輕輕混勻，在使用前一小時內(nèi)配制。 辣根過氧化物酶標記親和素的稀釋原則： 臨用前以辣根過氧化物酶標記親和素稀釋液稀釋，稀釋前根據(jù)預(yù)先計算好的每次實驗所需的總量配制（每孔 100 μ l ），實際配制時應(yīng)多配制 0.1-0.2ml 。如 10 μ l 辣根過氧化物酶標記親和素加 990 μ l 辣根過氧化物酶標記親和素稀釋液 的比例配制，輕輕混勻，在使用前一小時內(nèi)配制。

超氧化物岐化酶ELISA試劑盒

感謝各位對本公司的支持與認可，我們將再接再厲為您們提供的產(chǎn)品與服務(wù)。超氧化物岐化酶ELISA試劑盒外，還有更多相關(guān)實驗產(chǎn)品，標準品，細胞株和實驗技術(shù)服務(wù)等等前期后續(xù)服務(wù)。如果您對考慮購買的ELISA試劑盒還有任何疑問，都可以直接向我們咨詢。為您服務(wù)是我們的榮幸。

（編輯：tanxi）

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