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實(shí)驗(yàn)室化合物結(jié)構(gòu)測定之紫外可見吸收光譜法簡介

時間:2011/10/13閱讀:1026
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紫外可見吸收光譜法是基于在200nm-800nm光譜區(qū)域內(nèi)測定物質(zhì)的吸收光譜或在某波長處的吸光度值,對物質(zhì)進(jìn)行定性、定量或結(jié)構(gòu)分析的一種方法。該法又稱為紫外可見分光光度法。紫外可見吸收光譜屬于電子光譜,是由多原子分子的外層電子或價電子的躍遷而產(chǎn)生的。這種光譜可用于含有不飽和鍵的化合物,尤其含有共軛體系的化合物的分析和研究。雖然紫外吸收光譜基本上只能反映分子中發(fā)色團(tuán)和助色團(tuán)的特性,而不是反映整體分子的特性,但在化合物結(jié)構(gòu)測定中仍有重要作用。紫外可見吸收光譜用于定量分析是這種方法zui重要的用途。利用有色溶液對可見光的吸收來進(jìn)行定量分析的方法稱為比色分析,這是古老的分析方法之一,至今仍被人們廣泛采用。自1945年美國Beckman公司推出世界上*臺成熟的紫外可見分光光度商品儀器以后,紫外可見分光光度法帶到飛速發(fā)展。利用的光譜區(qū)從可見光區(qū)擴(kuò)展到近紫外區(qū),不僅適用于有色溶液的測定,也可以用于紫外光區(qū)有吸收的無色組分,為許多無機(jī)和有機(jī)物的分析提供了一種方便的手段。

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