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SIGMA現(xiàn)貨,SIGMA代理

微量移液器的實驗室簡單檢測與校準(zhǔn)

時間:2011/10/13閱讀:912
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乾思生物編輯整理:
(一)氣密性檢測
移液器吸滿液體后,手持垂直放置15s,檢查吸嘴的尖頭有無液滴,如有,則說明漏氣。
 
(二)準(zhǔn)確性檢測
1)量程小于1ul,建議使用分光光度法檢測。將移液器調(diào)至目標(biāo)體積,然后移取染料溶液,加入一定體積的蒸餾水中,測定溶液的稀釋度(334nm或340nm),重復(fù)幾次移液操作,計算移液器的度。
 
2)量程大于l/ul,可以用稱重法檢測。通過對水的稱重,轉(zhuǎn)換成體積(體積=質(zhì)量/密度),鑒別移液器的準(zhǔn)確性。由于水的密度是隨著溫度變化而變化,且稱量天平本身度不符合檢測要求,檢測又大多在一個開放式空間內(nèi)操作,偏差在所難免。因而,此種稱量法只能現(xiàn)場粗略地判斷移液器的準(zhǔn)確性,進一步的校準(zhǔn)必須在專業(yè)的實驗室操作進行。
 
注意:稱重法實驗室*條件是高度靈敏的分析天平(定期校準(zhǔn))、雙蒸水和稱量容器。水、移液器和吸嘴必須具有相同的溫度(20℃時水的密度為0.099 82)。 

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