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使用移液器問(wèn)題解答

時(shí)間:2011/10/13閱讀:676
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 乾思 生物編輯整理:

使用移液器問(wèn)題一:
移液器滲漏,多道移液器吸嘴里不均衡的液體水平
 
可能原因
·吸嘴與移液器吸嘴基部不配套
·當(dāng)裝吸嘴在多道移液器上時(shí)吸嘴架彎曲
·吸嘴接觸不緊密
 
提議
·換成質(zhì)量一致的吸嘴(例如: Expel PlusTM 吸嘴)
·換成更堅(jiān)固的吸嘴架
·稍用力擠壓吸嘴(然而不要用力過(guò)量)
 
使用移液器問(wèn)題二:
不準(zhǔn)確性,不性
 
可能原因
·移液器校準(zhǔn)不正確
·吸嘴質(zhì)量不夠高
·移液器基部的 O 型環(huán)與吸嘴內(nèi)環(huán)沖突
·吸嘴基部的 O 型環(huán)用舊了或損壞了
·活塞密封(內(nèi)部 O 型環(huán))損壞或用舊了
·吸嘴基部可能松了
·體積控制器的頂部旋可能松了
 
提議
·根據(jù)說(shuō)明書重新校準(zhǔn)
·換成質(zhì)量一致的吸嘴(例如: Expel PlusTM 吸嘴)
·換成沒(méi)有內(nèi)部密封環(huán)的吸嘴
·換 O 型環(huán)
·卸下吸嘴基部,換 O 型環(huán),檢查校準(zhǔn)
·拉緊吸嘴基部
·檢查旋鈕,上緊且必要時(shí)重新校準(zhǔn)
 
使用移液器問(wèn)題三:
液體殘留在吸嘴里
 
可能原因
·吸嘴可能是親水性的
 
提議
·使用疏水性(低保持力)吸嘴,例如: Expel PlusTM 吸嘴
 
使用移液器問(wèn)題四:
液體殘留在吸嘴里
 
可能原因
·活塞密封(內(nèi)部 O 型環(huán))損壞或用舊了
·分散于活塞上的油不均衡(可能由儲(chǔ)存過(guò)長(zhǎng),沒(méi)有垂直儲(chǔ)存移液器或在中間體積過(guò)度使用移液器等引起)
·活塞需要潤(rùn)滑油
·活塞上具有堆積物
 
提議
·卸下吸嘴基部,換 O 型環(huán),檢查校準(zhǔn)
·將移液器調(diào)至zui大體積(吸嘴:使用移液器隨機(jī)贈(zèng)送的固定體積移液器),使活塞充分上下來(lái)回 3-5 次
·根據(jù)說(shuō)明書潤(rùn)滑活塞
·清洗活塞并根據(jù)說(shuō)明書重新潤(rùn)滑
 
 
乾思 生物提醒:
·總是以直立(垂直)狀態(tài)儲(chǔ)存移液器。每天早晨通過(guò)在zui大體積處壓下活塞 4-5 次,預(yù)先準(zhǔn)備好移液器
·千萬(wàn)不要將帶有吸嘴(里面有液體)的移液器倒置或水平放置??偸钦_的裝置高質(zhì)量的吸嘴。

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