上海乾思生物科技有限公司
中級(jí)會(huì)員 | 第16年

18916423352

細(xì)胞庫(kù)
優(yōu)勢(shì)品牌
Encapsula NanoSciences Quidel Bioporto Salimetrics Research Products International Corp Quanta BioDesign Peninsula Laboratories International Golden West Diagnostics Ocean NanoTech HaemoScan affinitybiologicals Cytodiagnostics Innovagen AB CLIAwaived Svar Abbexa ABMgood AffinityImmuno AGScientific AHF analysentechnik AG Alomone 英國(guó)Antibodies AstaTech Avidity Badrilla BioAcademia Biomatik Biosynth BioTeZ Biotrend Calbiotech CaroteNature Cell Sciences Click Chemistry Tools Cohesion Biosciences CordenPharma Cosmo Bio Creative Biomart Crystal Chem Crystalgen Diagnovum DiaMetra Dianova Diatheva Eagle Manufacturing Echelon Biosciences Edgebio Emfret Epoch Life Science Equitech-Bio Eramask Euro Diagnostica Exocell Fine Science Tools GenwayBio Giotto Biotech Glen Research Glycosynth Gold Biotechnology Golden West Biologicals Haemtech(HTI) Herolab IHC WORLD ImmuChrom GmbH Immunostep Immunotools Immunovision INCELL Corporation Innovative Research Innovrsrch(IRA) Insitus Iris Biotech GmbH Isosep IST Engineering Inc J.G. Finneran Kainos Kamiya Biomedical Labor Diagnostika Nord Larodan Laysanbio Lc laboratories Lee BioSolutions LGC Biosearch Technologies Lifecore List Labs lumiprobe Macrocyclics Medix Biochemica Meridian Life Science Microbix Milenia Biotec Moscerdam Mybiosource Nanoimmunotech Nanoprobes Neuro Probe Paratechs Physitemp Polypure Polysciences Probetex Progen ProScience Inc ProSpec QAbio Qorpak Quanta BioSciences QuickZyme RevMAb Biosciences SCHMIDT BioMedTech Seven Hills Foundation Smartox Biotechnology Spherotech Surgimax Swant SYSY Ted Pella Torpac Trilink USBio VIDIA Ltd ViroStat Virusys VP-scientific Xcessbio XENOMETRIX Zeptometrix ZYTOVISION
自產(chǎn)ELISA試劑盒,試劑盒
進(jìn)口ELISA試劑盒
人細(xì)胞,人細(xì)胞系,人細(xì)胞株
3h biomedical,3H Biomedical AB,3hbiomedical,3-M Company,4adi,4rtilab,5 prime,5prime a & g pharmaceuticals,A&D Weighing,Aalto Scientific,Aalto Scientific ltd,Aalto Scientific,ltd.,aaltobioreagents,aaltoscientific,aat bioquest AAT Bioquest Inc,AB Biolabs,ab peptides,AB Sciex,Abaxis,Abazyme,Abberior,abbexa abbiotec,Abbkine,abbomax,Abbott Molecular,abcam,ABclonal,ABclonal Inc.,abcore-inc abcr GmbH,abcrGmbH,abcysonline,ABD,ABD Bioquest,Inc,abd serotec,ab-direct,abdserotec Abeomics,abfrontier,abgab,abgene,abgent,ABI Applied Biosystems,abiocode,abionline Abkem Iberia,ABL,Inc,Abliance,abm Inc.,abmart,abmgood,Abmole,Abmole Bioscience Inc. abnova,Abraxis,Absolute Antibody,ABT Beads,abt beads technologies,ABTbeads,abvision,Abways abzoom,academy biomed,academy bio-medical,acanthus research,acanthusresearch,ACC,accelagen,Accelagen inc. Access Biologicals,LLC,accessbiologicals,acciusa,accu-chek,AccuDiag Allergy,AccuDiag Anemia,AccuDiag Autoimmune Diseases,AccuDiag Bone Metabolism AccuDiag Thyroid,accumax accutase,Accumetrics,Accurate,accurate chemical,Accurate Wirecraft Company,accuratechemical,accustandard acdbio,Ace Glass,Ace Hose and Rubber Co,ACEA Biosciences,ACL Incorporated,ACME Microspheres Inc,ACMEmicrospheres,acris acris antibodies,Acris Antibodies GmbH,acris-antibodies,ACROBiosystems,acrometrix,acros,Acros Organics,ACT Test Panels LLC actc,actgene,active motif,activemotif,Adam Equipment,Inc,AddexBio,Addexbio Technologies,addgene ademtech,adg,ADI,ADInstruments,adipogen,Adolf Kuhner,adooq,Advance Tabco advanced immunochemical,advanced immunochemical inc,advanced targeting systems,Advansta,Advansta Inc.,Advansta,Inc,Advantech,Advent Electric Inc adventbio,advnt,advtechind,advy chemical,advy chemical Purified Proteins,advychemical Polyclonal Antisera,aesku,aetltd affiland,affinity,affinity biologicals,affinity bioreagent,Affinity BioReagents,Affinity BioReagents abr,Affinity Biosciences,affinitybiologicals AffinityBioReagents,AffinityImmuno,affix scientific,affixscientific,affymetrix,ag scientific,Agar Scientific,agarscientific agdia,Agela Technologies,agilent,Agilent Seahorse,agilent technologies,agilet,agilet solutions,agiletsolutions agrenvec,agrisera,AGRO-BIO,agscientific,ahdiagnostics,ahf,AHF analysentechnik,AHF analysentechnik AG Air Technology Solutions,Airgas Safety,Air-Tite,AJAX SCIENTIFIC LTD,AK Limited,Akro-Mils,ALA Scientfic,alamarblue Alba Bioscience,Alconox Inc,aldevron,alerchek,alere,Alexis,ALEXIS Biochemicals,Alfa Aesar Aline Biosciences,AlineBiosciences,All Foils Incorporated,Allcells,allele biotech,allelebiotech,allen-bradley,AllerMAbs
大鼠細(xì)胞,大鼠細(xì)胞系
細(xì)胞株,細(xì)胞系,傳代細(xì)胞
白鰱細(xì)胞 斑馬魚細(xì)胞 豹貓細(xì)胞 成人細(xì)胞 赤狐細(xì)胞 大額牛細(xì)胞 大耳山羊細(xì)胞 大黃魚細(xì)胞 大長(zhǎng)舌果蝠細(xì)胞 俄勒岡地鼠細(xì)胞 非洲綠猴細(xì)胞 高背鯽魚細(xì)胞 果蠅細(xì)胞 果子貍細(xì)胞 荷蘭黑白花奶牛細(xì)胞 黑化大家鼠細(xì)胞 花鼠細(xì)胞 家兔細(xì)胞 昆蟲細(xì)胞 綠猴細(xì)胞 馬鐵菊頭蝠細(xì)胞 綿羊細(xì)胞 三葉小蹄蝠細(xì)胞 散鱗鏡鯉細(xì)胞 社鼠細(xì)胞 鼠細(xì)胞 樹鼩細(xì)胞 團(tuán)頭魴細(xì)胞 新生兒細(xì)胞 新生牛細(xì)胞 敘利亞倉(cāng)鼠細(xì)胞 長(zhǎng)尾綠猴細(xì)胞 中國(guó)倉(cāng)鼠細(xì)胞 中華鱉細(xì)胞 棕果蝠細(xì)胞 腫瘤細(xì)胞 豬細(xì)胞 兔細(xì)胞 犬細(xì)胞
抗體庫(kù),小包裝,流式抗體
SIGMA現(xiàn)貨,SIGMA代理

移液器的檢測(cè)和校準(zhǔn)指南

時(shí)間:2011/10/13閱讀:539
分享:
乾思 生物編輯整理:
一、實(shí)驗(yàn)室簡(jiǎn)單檢測(cè)
(一)氣密性檢測(cè)
移液器吸滿液體后,手持垂直放置15s,檢查吸嘴的尖頭有無(wú)液滴,如有,則說(shuō)明漏氣。
(二)準(zhǔn)確性檢測(cè)
1)量程小于1μl,建議使用分光光度法檢測(cè)。將移液器調(diào)至目標(biāo)體積,然后移取染料溶液,加入一定體積的蒸餾水中,測(cè)定溶液的稀釋度(334nm或340nm),重復(fù)幾次移液操作,計(jì)算移液器的度。
2)量程大于1μl,可以用稱重法檢測(cè)。通過(guò)對(duì)水的稱重,轉(zhuǎn)換成體積(體積=質(zhì)量/密度),鑒別移液器的準(zhǔn)確性。由于水的密度是隨著溫度變化而變化,且稱量天平本身度不符合檢測(cè)要求,檢測(cè)又大多在一個(gè)開放式空間內(nèi)操作,偏差在所難免。因而,此種稱量法只能現(xiàn)場(chǎng)粗略地判斷移液器的準(zhǔn)確性,進(jìn)一步的校準(zhǔn)必須在專業(yè)的實(shí)驗(yàn)室操作進(jìn)行。
注意:稱重法實(shí)驗(yàn)室*條件是高度靈敏的分析天平(定期校準(zhǔn))、雙蒸水和稱量容器。水、移液器和吸嘴必須具有相同的溫度(20℃時(shí)水的密度為0.09982)。
二、專業(yè)校準(zhǔn)
移液器的工作量大,長(zhǎng)期使用,將會(huì)使彈簧彈力發(fā)生變形,加之本身是塑料,不耐磨擦,就會(huì)產(chǎn)生誤差。為了保證移液器傳遞液體量的準(zhǔn)確性,必須對(duì)移液器進(jìn)行定期校準(zhǔn)。為了更好地發(fā)揮該類計(jì)量?jī)x器的作用,對(duì)新購(gòu)進(jìn)的儀器要進(jìn)行校準(zhǔn)才能使用;對(duì)正在使用的計(jì)量?jī)x器,由于長(zhǎng)期使用會(huì)造成其示值于度量對(duì)象的誤差,這種誤差若不進(jìn)行控制,及時(shí)校正,必定會(huì)影響科研工作。因此,對(duì)使用的移液器要定期檢定、校正,并建立檔案,只有這樣才能使其在日常工作中更好的發(fā)揮作用。

會(huì)員登錄

×

請(qǐng)輸入賬號(hào)

請(qǐng)輸入密碼

=

請(qǐng)輸驗(yàn)證碼

收藏該商鋪

X
該信息已收藏!
標(biāo)簽:
保存成功

(空格分隔,最多3個(gè),單個(gè)標(biāo)簽最多10個(gè)字符)

常用:

提示

X
您的留言已提交成功!我們將在第一時(shí)間回復(fù)您~
撥打電話
在線留言