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細(xì)胞培養(yǎng)中常用培養(yǎng)基

時(shí)間:2011/10/25閱讀:639
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1、RPMI-1640 Medium,RPMI-1640廣泛應(yīng)用于哺乳動(dòng)物、特殊造血細(xì)胞、正常或惡性增生的白細(xì)胞,雜交瘤細(xì)胞的培養(yǎng),是目前應(yīng)用十分廣泛的培養(yǎng)基。主要用于懸浮細(xì)胞培養(yǎng)。其它像K-562、HL-60、Jurkat、Daudi、IM-9等成淋巴細(xì)胞、T細(xì)胞淋巴瘤細(xì)胞以及HCT-15上皮細(xì)胞等均可參考使用。2、Minimum Essential Medium(MEM),也稱(chēng)zui低必需培養(yǎng)基,它僅含有12種必需氨基酸、谷氨酰胺和8種維生素。成分簡(jiǎn)單,可廣泛適應(yīng)各種已建成細(xì)胞系和不同地方的哺乳動(dòng)物細(xì)胞類(lèi)型的培養(yǎng)。MEM-Alpha一般用于培養(yǎng)一些難培養(yǎng)細(xì)胞類(lèi)型,而其它沒(méi)有特殊之處的細(xì)胞株則幾乎均可采用MEM來(lái)培養(yǎng)。3、DMEM-高糖(標(biāo)準(zhǔn)型)是一種應(yīng)用十分廣泛的培養(yǎng)基,可用于許多哺乳動(dòng)物細(xì)胞培養(yǎng),更適合高密度懸浮細(xì)胞培養(yǎng)。適用于附著性較差,但又不希望它脫離原來(lái)生長(zhǎng)點(diǎn)的克隆培養(yǎng),也可用于雜交瘤中骨髓瘤細(xì)胞和DNA轉(zhuǎn)染的轉(zhuǎn)化細(xì)胞的培養(yǎng)。4、DMEM-低糖(標(biāo)準(zhǔn)型)是一種應(yīng)用十分廣泛的培養(yǎng)基,可用于許多哺乳動(dòng)物細(xì)胞培養(yǎng)。低糖適于依賴(lài)性貼壁細(xì)胞培養(yǎng),特別適用于生長(zhǎng)速度快、附著性較差的腫瘤細(xì)胞培養(yǎng)。5、DMEM/F12,DMEM/F12培養(yǎng)基適于克隆密度的培養(yǎng)。F12培養(yǎng)基成分復(fù)雜,含有多種微量元素,和DMEM以1:1結(jié)合,稱(chēng)為DMEM/F12培養(yǎng)基(DME/F12medium),作為開(kāi)發(fā)無(wú)血清配方的基礎(chǔ),以利用F12含有較豐富的成分和DMEM含有較高濃度的營(yíng)養(yǎng)成分為優(yōu)點(diǎn)。該培養(yǎng)基適用于血清含量較低條件下哺乳動(dòng)物細(xì)胞培養(yǎng)。為了增強(qiáng)該培養(yǎng)基的緩沖能力,改良之一是在DMEM/F12(1:1)中加入15mMHEPES緩沖液。6、McCoy’s ,McCoy’s Medium 主要為肉瘤細(xì)胞的培養(yǎng)所設(shè)計(jì),可支持多種(如骨髓、皮膚、肺和脾臟等)的原代移植物的生長(zhǎng),除適于一般的原代細(xì)胞培養(yǎng)外,主要用于作組織活檢培養(yǎng)、一些淋巴細(xì)胞培養(yǎng)以及一些難培養(yǎng)細(xì)胞的生長(zhǎng)支持。例如Jensen大鼠肉瘤成纖維細(xì)胞、人淋巴細(xì)胞、HT-29、BHL-100等上皮細(xì)胞。7、Iscove’s Modified Dulbecco Medium (IMDM),Guilber 和Iscove將Dulbecco’Medium 改良為 Iscove’s Medium,用于培養(yǎng)紅細(xì)胞和巨噬細(xì)胞前體。此種培養(yǎng)液含有硒、額外的氨基酸和維生素、丙酮酸鈉和HEPES。并用硝酸鉀取代了硝酸鐵。IMDM還能夠促進(jìn)小鼠B淋巴細(xì)胞,LPS刺激的B細(xì)胞,骨髓造血細(xì)胞,T細(xì)胞和淋巴瘤細(xì)胞的生長(zhǎng)。IMDM為營(yíng)養(yǎng)非常豐富的培養(yǎng)液,因此可以用于高密度細(xì)胞的快速增殖培養(yǎng)。8、M-199 Medium,1950年,Morgan成功研制出具有確定化學(xué)成分的細(xì)胞培養(yǎng)液,即M-199,主要用于雞胚成纖維細(xì)胞培養(yǎng)。此培養(yǎng)液必須輔以血清才能支持長(zhǎng)期培養(yǎng)。M-199可用于培養(yǎng)多種種屬來(lái)源的細(xì)胞,并能培養(yǎng)轉(zhuǎn)染的細(xì)胞。9、Leibovitz Medium (L-15),L-15培養(yǎng)液適用于快速增殖瘤細(xì)胞的培養(yǎng),用于在CO2缺乏的情況下培養(yǎng)腫瘤細(xì)胞株。此培養(yǎng)液采用磷酸鹽緩沖體系,氨基酸組成進(jìn)一步改良,并由半乳糖替代了葡萄糖。10、Ham’s F-10培養(yǎng)基:適應(yīng)小鼠細(xì)胞、人類(lèi)二倍體的培養(yǎng)。11、Ham’s F-12培養(yǎng)基:可以在加入很少血清的情況下應(yīng)用,特別適合單細(xì)胞培養(yǎng)和克隆化培養(yǎng),是無(wú)血清培養(yǎng)中常用的基礎(chǔ)培養(yǎng)液。12、William’s Medium E:用于大鼠肝上皮細(xì)胞的長(zhǎng)期細(xì)胞培養(yǎng)。13、MCDB 131 培養(yǎng)液:用于培養(yǎng)內(nèi)皮細(xì)胞。14、Opti-MEM I Reduced Serum Media:用于培養(yǎng)造血細(xì)胞。15、植物血凝素(PHA):增加細(xì)胞轉(zhuǎn)化和DNA合成。

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