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SIGMA現(xiàn)貨,SIGMA代理

近年來新建立的與補體有關(guān)的血清學(xué)試驗

時間:2011/10/26閱讀:588
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(一) 單擴散溶血試驗(single radial hemolysis) 又稱固相溶血試驗。根據(jù)抗原(如綿羊紅細(xì)胞)與相應(yīng)抗體相遇形成的免疫復(fù)合物,可通過傳統(tǒng)途徑激活補體,使綿羊紅細(xì)胞發(fā)生溶血,其溶血程度與特異抗體滴度成正比的原理設(shè)計而成。現(xiàn)以鼻疽為例說明其操作原則。取鼻疽抗原致敏的綿羊紅細(xì)胞加入融化的瓊脂糖凝膠中,混合均勻,澆注反應(yīng)盤,制成致敏的反應(yīng)盤,打孔,隨后將被檢血清和補體的等量混合物加入到上述致敏反應(yīng)盤孔中,于室溫下感作3~4小時,陽性者,在孔的周圍發(fā)生明顯的溶血環(huán),其直徑在8.1mm以上。(二) 固相補體結(jié)合試驗(solid phase CFT) 系根據(jù)直接補體結(jié)合反應(yīng)的原理設(shè)計而成,只不過用瓊脂糖凝膠代替生理鹽水作介質(zhì)實施反應(yīng),即將鼻疽抗原、被檢血清與補體等量混合,放恒溫箱中感作1小時后,取混合物滴加于預(yù)先制備好的含溶血素致敏綿羊紅細(xì)胞的瓊脂糖凝膠的相應(yīng)孔中,與此同時,每份血清必須作一個不加抗原的對照孔,再置恒溫箱中感作3小時,然后根據(jù)每份血清對照孔與試驗孔之間發(fā)生的溶血環(huán)直接差的大小,確定檢驗結(jié)果,凡在0.6mm以上者,判為鼻疽陽性。

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