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培養(yǎng)基的鑒別

時(shí)間:2011/11/1閱讀:693
分享:
一、EMB培養(yǎng)基:常用于鑒別E.coli  蛋白胨10g 乳糖5g 蔗糖5g 磷酸氫二鉀2g 伊紅Y 0.4g 美藍(lán)0.065g 蒸餾水1000g pH7.2
二、2216E培養(yǎng)基配方: 分離海洋微生物的培養(yǎng)基配方 ,蛋白胨 5g 酵母膏1g 磷酸高鐵 0.01g 瓊脂15~20g 陳海水 1000ml 煮沸氫氧化鈉(5%)的溶液調(diào)PH值7.6~7.8.
三、伊紅美藍(lán)培養(yǎng)基:可用于檢測水中大腸桿菌的含量,首先按以下組成配制基礎(chǔ)培養(yǎng)基,蛋白胨10g NaCl 5g,瓊脂15~20g.
   將上述物質(zhì)溶解后,用蒸餾水定容至1000mL,調(diào)節(jié)pH為7.6.滅菌后,冷卻至60℃左右,再按下面的比例,在無菌操作條件下加入滅菌的乳糖溶液、伊紅水溶液以及美藍(lán)水溶液。搖勻后,立即倒平板。溶液中的乳糖在高溫下會(huì)破壞,因此一般使用壓力為70kPa、溫度為115℃的條件滅菌20min.
四、基礎(chǔ)培養(yǎng)基 100mL 20%乳糖溶液2mL :2%伊紅水溶液 2mL 0.5%美藍(lán)水溶液1mL.

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