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SIGMA現(xiàn)貨,SIGMA代理

培養(yǎng)基的質(zhì)量判斷

時(shí)間:2011/11/4閱讀:952
分享:
1.外型:顏色、粉末細(xì)度都應(yīng)該均勻。
2.溶解性:培養(yǎng)基能夠*溶解,這樣可以避免培養(yǎng)基內(nèi)營養(yǎng)成分的流失。
3.pH值:pH值的測定也可以檢查培養(yǎng)基的批間差異。
4.水分:培養(yǎng)基的營養(yǎng)成份很豐富,利于微生物的滋長,因此水分的含量控制可以延長有效期。
5.冰點(diǎn)滲透壓:細(xì)胞必須生活在合適的滲透壓環(huán)境中;同時(shí)滲透壓值的測定也可以檢查培養(yǎng)基的批間差異。
6.菌落總數(shù):粉末培養(yǎng)基不是無菌制劑,培養(yǎng)基本身的營養(yǎng)成分很豐富,容易滋生微生物,因此菌落的控制可以延長有效期
7.細(xì)胞生長試驗(yàn):可檢查細(xì)胞培養(yǎng)基是否有促生長的能力,以及是否有不利細(xì)胞生長的毒素。
8.細(xì)菌內(nèi)毒素:為滿足生物制品細(xì)菌內(nèi)毒素的控制要求,作為生物制品生產(chǎn)原料的培養(yǎng)基同樣需要細(xì)菌內(nèi)毒素控制。
9.穩(wěn)定性:確定產(chǎn)品的儲(chǔ)存、運(yùn)輸條件,產(chǎn)品的保質(zhì)期限。
10.一致性:驗(yàn)證產(chǎn)品的均一性。

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