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SIGMA現(xiàn)貨,SIGMA代理

ELISA試劑盒的清洗

時(shí)間:2011/11/4閱讀:694
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 清洗ELISA試劑盒自備材料:

1.蒸餾水。   
2.加樣器:5ul、10ul、50ul、100ul、200ul、500ul、1000ul。 
3.振蕩器及磁力攪拌器等。
ELISA試劑盒操作注意事項(xiàng):
1.試劑應(yīng)按標(biāo)簽說(shuō)明書(shū)儲(chǔ)存,使用前恢復(fù)到室溫。稀稀過(guò)后的標(biāo)準(zhǔn)品應(yīng)丟棄,不可保存。  
2.實(shí)驗(yàn)中不用的板條應(yīng)立即放回包裝袋中,密封保存,以免變質(zhì)。 
3.不用的其它試劑應(yīng)包裝好或蓋好。不同批號(hào)的試劑不要混用。保質(zhì)前使用。   
4.使用一次性的吸頭以免交叉污染,吸取終止液和底物A、B液時(shí),避免使用帶金屬部分的加樣器。   
5.使用干凈的塑料容器配置洗滌液。使用前充分混勻試劑盒里的各種成份及樣品。   
6.洗滌酶標(biāo)板時(shí)應(yīng)充分拍干,不要將吸水紙直接放入酶標(biāo)反應(yīng)孔中吸水。   
7.底物A應(yīng)揮發(fā),避免長(zhǎng)時(shí)間打開(kāi)蓋子。底物B對(duì)光敏感,避免長(zhǎng)時(shí)間暴露于光下。避免用手接觸,有毒。實(shí)驗(yàn)完成后應(yīng)立即讀取OD值。  
8.加入試劑的順序應(yīng)一致,以保證所有反應(yīng)板孔溫育的時(shí)間一樣。  
9.按照說(shuō)明書(shū)中標(biāo)明的時(shí)間、加液的量及順序進(jìn)行溫育操作。
安全性:
1.避免直接接觸終止液和底物A、B。一旦接觸到這些液體,請(qǐng)盡快用水沖洗。
2.實(shí)驗(yàn)中不要吃喝、抽煙或使用化妝品。
3.不要用嘴吸取試劑盒里的任何成份。

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