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SIGMA現(xiàn)貨,SIGMA代理

ELISA檢測技術(shù)的優(yōu)勢

時間：2011/11/15閱讀：550
分享：

   近二十幾年來，免疫學(xué)分析方法發(fā)展突飛猛進，尤其是在使用標(biāo)記了的抗原和抗體的分析技術(shù)以后，使原來許多經(jīng)典的分析方法在敏感性和特異性方面都不能相比。繼50年代的免疫熒光（IFA）和60年代的放射免疫（RIA）分析技術(shù)之后，在70年代初期又建立了用酶來標(biāo)記抗原或抗體的分析技術(shù)。由于酶的生物催化作用，一個酶分子在數(shù)分鐘內(nèi)可以催化幾十幾百個底物分子發(fā)生反應(yīng)，產(chǎn)生了放大作用，使得原來極其微乎其微的抗原或抗體在數(shù)分鐘后就可被識別出來，這種技術(shù)被稱為酶聯(lián)免疫吸附試驗法（Enzyme—Linked Immunosorbent Assay，ELISA），本法自70年代初期由VAN WEEMEN、SCHUARS.ENGVALL及PERLMARN等相繼分別報道后，現(xiàn)已引起廣泛重視。ELISA法是免疫診斷中的一項新技術(shù)，現(xiàn)已成功地應(yīng)用于多種病原微生物所引起的傳染病、寄生蟲病及非傳染病等方面的免疫診斷。也已應(yīng)用于大分子抗原和小分子抗原的定量測定，根據(jù)已經(jīng)使用的結(jié)果，認(rèn)為ELISA法具有靈敏、特異、簡單、快速、穩(wěn)定及易于自動化操作等特點。不僅適用于臨床標(biāo)本的檢查，而且由于一天之內(nèi)可以檢查幾百甚至上千份標(biāo)本,因此，也適合于血清流行病學(xué)調(diào)查。本法不僅可以用來測定抗體，而且也可用于測定體液中的循環(huán)抗原，所以也是一種早期診斷的良好方法。因此ELISA法在生物醫(yī)學(xué)各領(lǐng)域的應(yīng)用范圍日益擴大，可概括四個方面：

1.免疫酶染色各種細胞內(nèi)成份的定位。 
2.研究抗酶抗體的合成。 
3.顯現(xiàn)微量的免疫沉淀反應(yīng)。 
4.定量檢測體液中抗原或抗體成份。 
酶聯(lián)免疫吸附試驗法以其靈敏度高（可達 ng 甚至pg 水平），特異性好等優(yōu)點，在生命科學(xué)各領(lǐng)域得到廣泛應(yīng)用，它已邁出醫(yī)藥、臨床領(lǐng)域，步入農(nóng)業(yè)、漁業(yè)、畜牧業(yè)和食品加工業(yè)。

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