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大腸桿菌宿主殘留蛋白試劑盒熱賣

時間：2013-7-10閱讀：963
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大腸桿菌宿主殘留蛋白試劑盒熱賣

大腸桿菌宿主殘留蛋白檢測試劑盒（E.coli P）ELISA試劑盒的詳細資料：

中文名稱：大腸桿菌宿主殘留蛋白檢測試劑盒（E.coli P）ELISA試劑盒

貨號：

規(guī)格：96T/48T

特別服務(wù)：可免費代檢測

供貨方式：現(xiàn)貨供應(yīng)

英文名稱：Escherichia coli Protein,E.coli P ELISA Kit

產(chǎn)品編號：RBE1655Ec

檢驗方法：ELISA

包 裝：96T

品 牌：Uscnlife

產(chǎn)品說明：大腸桿菌宿主殘留蛋白（E.coli P）ELISA Kit 概述

預(yù)期應(yīng)用

ELISA法定量測定生物制品或其它相關(guān)制品中大腸桿菌宿主殘留蛋白含量。

實驗原理

用純化的抗體包被微孔板，制成固相抗體，往微孔中依次加入標本、生物素化的抗宿主殘留蛋白抗體、HRP標記的親和素，經(jīng)過*洗滌后用底物TMB顯色。TMB在過氧化物酶的催化下轉(zhuǎn)化成藍色，并在酸的作用下轉(zhuǎn)化成zui終的黃色。顏色的深淺和樣品中的大腸桿菌宿主殘留蛋白呈正相關(guān)。用酶標儀在450nm波長下測定吸光度（OD值），計算樣品濃度。

大腸桿菌宿主殘留蛋白試劑盒實驗原理

本試劑盒應(yīng)用雙抗體夾心法測定標本中大腸桿菌宿主殘留蛋白（E.coli P）水平。用純化的大腸桿菌宿主殘留蛋白（E.coli P）抗體包被微孔板，制成固相抗體，往包被單抗的微孔中依次加入大腸桿菌宿主殘留蛋白（E.coli P），再與HRP標記的大腸桿菌宿主殘留蛋白（E.coli P）抗體結(jié)合，形成抗體-抗原-酶標抗體復(fù)合物，經(jīng)過*洗滌后加底物TMB顯色。TMB在HRP酶的催化下轉(zhuǎn)化成藍色，并在酸的作用下轉(zhuǎn)化成zui終的黃色。顏色的深淺和樣品中的大腸桿菌宿主殘留蛋白（E.coli P）呈正相關(guān)。用酶標儀在450nm波長下測定吸光度（OD值），通過標準曲線計算樣品中大腸桿菌宿主殘留蛋白（E.coli P）濃度。

乾思生物整理編輯： 進口elisa試劑盒，國產(chǎn)elisa試劑盒，elisa試劑盒，試劑盒，dna提取試劑盒，免疫組化試劑盒，rna提取試劑盒，質(zhì)粒提取試劑盒，dna試劑盒

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