人皮質(zhì)酮CORT酶聯(lián)免疫分析試劑盒實(shí)驗(yàn)原理

人皮質(zhì)酮CORT酶聯(lián)免疫分析試劑盒實(shí)驗(yàn)原理：本試劑盒用于測定人血清，細(xì)胞上清及相關(guān)液體樣本中皮質(zhì)酮 (CORT)的含量。應(yīng)用雙抗體夾心法測定標(biāo)本中人皮質(zhì)酮 (CORT)水平。用純化的人皮質(zhì)酮 (CORT)抗體包被微孔板，制成固相抗體，往包被單抗的微孔中依次加入皮質(zhì)酮 (CORT)，再與HRP標(biāo)記的皮質(zhì)酮 (CORT)抗體結(jié)合，形成抗體-抗原-酶標(biāo)抗體復(fù)合物，經(jīng)過*洗滌后加底物TMB顯色。TMB在HRP酶的催化下轉(zhuǎn)化成藍(lán)色，并在酸的作用下轉(zhuǎn)化成zui終的黃色。顏色的深淺和樣品中的人皮質(zhì)酮 (CORT)呈正相關(guān)。用酶標(biāo)儀在450nm波長下測定吸光度（OD值），通過標(biāo)準(zhǔn)曲線計(jì)算樣品中人皮質(zhì)酮 (CORT)濃度。

Human Corticosterone (CORT) ELISA Kit

Principle of the assay

The kit assay Human CORT level in the sample，use Purified Human CORT to coat microtiter plate wells, make solid-phase antibody, then add CORT to wells, Combined antibody which With HRP labeled goat anti-Mouse become antibody - antigen - enzyme-antibody complex, after washing Compley, Add TMB substrate solution,TMB substrate becomes blue color At HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of CORT in the samples is then determined by comparing the O.D. of the samples to the standard curve.

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