flexcell細(xì)胞應(yīng)力加載培養(yǎng)2019新文獻(xiàn)

flexcell細(xì)胞應(yīng)力加載培養(yǎng)2019新文獻(xiàn)

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[3] Bianchi F, George JH, Malboubi M, Jerusalem A, Thompson MS, Ye H. Engineering a uniaxial substrate-stretching device for simultaneous electrophysiological measurements and imaging of strained peripheral neurons. Medical engineering & physics. 2019;67:1-10.

[4] Boyle ST, Kular J, Nobis M, Ruszkiewicz A, Timpson P, Samuel MS. Acute compressive stress activates RHO/ROCK-mediated cellular processes. Small GTPases. 2018:1-17.

[5] Dolzani P, Assirelli E, Pulsatelli L, Meliconi R, Mariani E, Neri S. Ex vivo physiological compression of human osteoarthritis cartilage modulates cellular and matrix components. PloS one. 2019;14:e0222947.

[6] Fang B, Liu Y, Zheng D, Shan S, Wang C, Gao Y, et al. The effects of mechanical stretch on the biological characteristics of human adipose-derived stem cells. Journal of cellular and molecular medicine. 2019;23:4244-55.

[7] Friedrich O, Merten AL, Schneidereit D, Guo Y, Schurmann S, Martinac B. Stretch in Focus: 2D Inplane Cell Stretch Systems for Studies of Cardiac Mechano-Signaling. Frontiers in bioengineering and biotechnology. 2019;7:55.

[8] He YB, Liu SY, Deng SY, Kuang LP, Xu SY, Li Z, et al. Mechanical Stretch Promotes the Osteogenic Differentiation of Bone Mesenchymal Stem Cells Induced by Erythropoietin. Stem cells international. 2019;2019:1839627.

[9] Hilscher MB, Sehrawat T, Arab JP, Zeng Z, Gao J, Liu M, et al. Mechanical Stretch Increases Expression of CXCL1 in Liver Sinusoidal Endothelial Cells to Recruit Neutrophils, Generate Sinusoidal Microthombi, and Promote Portal Hypertension. Gastroenterology. 2019;157:193-209 e9.

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美國Flexcell公司，成立于1987年，該公司專注于細(xì)胞力學(xué)培養(yǎng)產(chǎn)品的設(shè)計和制造。以提供*的體外細(xì)胞拉應(yīng)力、壓應(yīng)力和流體剪切應(yīng)力加載刺激系統(tǒng)以及配套的培養(yǎng)板、硅膠膜載片等耗材*。

Flexcell細(xì)胞組織力學(xué)培養(yǎng)系統(tǒng)不僅能對各種2D、3D細(xì)胞組織提供拉應(yīng)力、壓應(yīng)力、切應(yīng)力刺激加載,而且還可以提供拉應(yīng)力和切應(yīng)力混合力同時加載;不僅能對細(xì)胞組織進(jìn)行機械力加載刺激,而且還能進(jìn)行三維培養(yǎng)、人工生物組織構(gòu)建、動力模擬;不僅能單軸向牽張拉伸,而且還可以雙軸向牽張拉伸;不僅可以根據(jù)實驗需要調(diào)節(jié)受力大小,而且還可以模擬不同受力時間。

Flexcell*的StageFlexer拉應(yīng)力顯微設(shè)備、StagePresser壓應(yīng)力顯微設(shè)備、Flex Flow切應(yīng)力顯微設(shè)備，可在加力培養(yǎng)的同時實時觀察研究細(xì)胞組織在力作用下的反應(yīng)變化；*的flexstop隔離閥能使同一塊培養(yǎng)板里的細(xì)胞組織一部分受力，一部分不受力，方便進(jìn)行對比實驗

這些系統(tǒng)智能、精準(zhǔn)誘導(dǎo)來自各種細(xì)胞、組織在拉應(yīng)力、壓應(yīng)力和流體切應(yīng)力作用下發(fā)生的生化生理變化，專業(yè)、細(xì)膩的闡釋了體外細(xì)胞、組織機械力刺激加載、力學(xué)信號感受和響應(yīng)機制。對研究細(xì)胞的形態(tài)結(jié)構(gòu)及功能，細(xì)胞的生長、發(fā)育、成熟、增殖、衰老、凋亡、死亡及癌變以及通路表達(dá)，細(xì)胞信號傳導(dǎo)及基因表達(dá)的調(diào)控，細(xì)胞的分化及其調(diào)控機理具有重要意義。

細(xì)胞組織應(yīng)力背景與作用
生命活動中無論是心臟的博動、動脈的收縮和舒張、腸道的蠕動，骨生長正畸，肌肉生長正畸，血管蠕動，肢體運動，器官活動，還是胸肺的呼吸都不斷地對參與其中的細(xì)胞施加動態(tài)的應(yīng)力（拉應(yīng)力、壓應(yīng)力、切應(yīng)力）作用。因此正確理解細(xì)胞對外應(yīng)力刺激行為對骨肉正畸、肌肉收縮、創(chuàng)傷修復(fù)、腫瘤轉(zhuǎn)移、器官組織康復(fù)等許多重要生物醫(yī)學(xué)領(lǐng)域都有十分重要的意義

應(yīng)力信號協(xié)同生物化學(xué)信號是生物自適應(yīng)結(jié)構(gòu)自我設(shè)計和調(diào)控長成的設(shè)計和調(diào)控者，細(xì)胞核是細(xì)胞代謝活動的控制中心，指揮它的活動除了遺傳密碼外主要是外部刺激傳來的信號。細(xì)胞處于組織的應(yīng)力環(huán)境中，應(yīng)力刺激細(xì)胞膜并通過微絲和微管傳遞到細(xì)胞核，應(yīng)力信號在傳遞過程中引起一系列生化反應(yīng)。研究成果已證明應(yīng)力信號與化學(xué)信號在決定細(xì)胞活動中具有同等重要性，應(yīng)力信號在調(diào)控細(xì)胞的分化、生長和凋亡中起著主導(dǎo)作用。應(yīng)力刺激按作用方向分為張應(yīng)力、壓應(yīng)力和切應(yīng)力（血流對管壁）等，按時間分為定常和脈動應(yīng)力。 
研究確認(rèn)應(yīng)力是調(diào)控功能細(xì)胞的決定性因素 
應(yīng)力仿真加載模擬膜型是細(xì)胞力學(xué)研究面臨的shou要問題
由于生物體內(nèi)器官和組織結(jié)構(gòu)極其復(fù)雜，生物個體也存在較大差異，致使在體細(xì)胞的力學(xué)環(huán)境復(fù)雜多樣，從而增加在體細(xì)胞力學(xué)行為研究的難度。由于生物體內(nèi)的細(xì)胞、細(xì)胞膜極小，宏觀力學(xué)加載方法和實驗技術(shù)無法直接使用，因此，尋找合適細(xì)胞力學(xué)加載方法和能膜擬生命體內(nèi)細(xì)胞組織生長生物力環(huán)境的細(xì)胞組織體外機械力加載裝置，實現(xiàn)體外分離和建立合適的加載膜型是細(xì)胞力學(xué)研究面臨的要問題。美國Fexcell®研制的體外細(xì)胞組織拉應(yīng)力、壓應(yīng)力、和流體剪切力加載仿真模擬模型系統(tǒng)智能、精準(zhǔn)誘導(dǎo)來自各種細(xì)胞、組織在拉力、壓力和流體切應(yīng)力等體外機械力刺激作用下發(fā)生的生化生理變化，專業(yè)、細(xì)膩的闡釋了體外細(xì)胞、組織機械力刺激加載、力學(xué)信號感受和響應(yīng)機制。國內(nèi)外有近3000篇成功應(yīng)用文獻(xiàn)案例，是細(xì)胞組織力學(xué)研究者的shou選。 
Flexcell總授權(quán)代理
世聯(lián)博研北京科技有限公司是Flexcell細(xì)胞力學(xué)設(shè)備與耗材在中國大陸、香港、澳門、馬來西亞、新加波區(qū)域總授權(quán)代理商， 為廣大科研用戶提供flexcell全系列產(chǎn)品。