B淋巴瘤細(xì)胞B-lymphoma cells懸浮細(xì)胞如何固定免疫刺激解決方案？

B-lymphoma cells懸浮細(xì)胞，B細(xì)胞淋巴瘤是B細(xì)胞發(fā)生的實(shí)體腫瘤。包括霍奇金淋巴瘤和非霍奇金淋巴瘤。B細(xì)胞淋巴腫瘤是一種起源于B淋巴細(xì)胞的惡性腫瘤，也被稱(chēng)為非霍奇金淋巴瘤（NHL）。它是淋巴瘤的一種常見(jiàn)類(lèi)型，約占所有淋巴瘤的30-40%。B細(xì)胞淋巴腫瘤的特點(diǎn)是異常B細(xì)胞在淋巴結(jié)或其他器官中異常增生，并可能影響患者的免疫功能。

研究懸浮細(xì)胞時(shí)，我們經(jīng)常需要做免疫熒光，但是懸浮細(xì)胞如何固定？一直困擾著我們。如何像貼壁細(xì)胞一樣處理懸浮細(xì)胞，避免掉片或者細(xì)胞固定不住，痛點(diǎn)解決方案來(lái)了。靶點(diǎn)科技Shi-Fix coverslips可以固定懸浮細(xì)胞，僅僅需要四步。

B-lymphoma cells如何固定？并且固定還可以刺激培養(yǎng)，可以參考如下解決方案。

Evaluation of cellular uptake of gold nanoparticles under a confocal microscope

B-lymphoma cells (5?×?105 cells/mL) were seeded in Shi-fix coverslips (Shikhar Biotech) and incubated in RPMI 1640 medium supplied with 10% FBS under a 5% CO2 atmosphere. After 24?h of incubation, the cells were washed twice with PBS and treated with FITC-labeled NK20a peptide (NK20a-FITC) or NK20a-FITC@Au nanoparticles for 1?h at 37?°C. For visualization, the cell membranes were stained with Cytopainter (ab219942, Abcam, Cambridge, UK), and the nuclei were stained with 4’,6-diamidino-2-phenylindole (DAPI). The samples were each treated with 100?µL of Cytopainter for 20?min, then washed with fresh PBS. Subsequently, the cells were fixed with 4% glutaraldehyde for another 20?min. After the fixation, 3–4 drops of mounting medium with 0.0002% DAPI (ab104139, Abcam, Cambridge, UK) were applied directly to the samples to stain the nuclei of cells. The prepared samples were imaged with a confocal fluorescence microscope (C2si?+?, Nikon, Tokyo, Japan).