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GeneTex熱銷抗體 — GAPDH抗體原理/介紹

來源：深圳欣博盛生物科技有限公司 2024年12月13日 17:41

GAPDH抗體識別甘油醛-3-磷酸脫氫酶(GAPDH)蛋白，相基因的產(chǎn)物催化碳水化合物代謝中一個(gè)重要的能量產(chǎn)生步驟，即在無機(jī)磷酸鹽和煙酰胺腺嘌呤二核苷酸（NAD）存在的情況下，對甘油醛-3-磷酸進(jìn)行可逆的氧化磷酸化。GeneTex熱銷抗體 —— GAPDH抗體（克隆號GT239）助理您的科研實(shí)驗(yàn)！

產(chǎn)品詳情

產(chǎn)品名稱：GAPDH antibody [GT239]

別名：glyceraldehyde-3-phosphate dehydrogenase , G3PD , GAPD , HEL-S-162eP

反應(yīng)種屬：Candida albicans, Chinese Hamster, Dog, Drosophila, E. coli, Hamster, Human, Monkey, Mouse, Pig, Rat, Yeast, Zebrafish

宿主來源：Mouse

實(shí)驗(yàn)應(yīng)用：EMSA, ICC/IF, IHC-P, WB

同種型：IgG2b

免疫原：Recombinant protein encompassing a sequence within the center region of human GAPDH. The exact sequence is proprietary.

克隆性：Monoclonal

克隆號：GT239

純化方式：Affinity purified by Protein G.

偶聯(lián)：Unconjugated

產(chǎn)品濃度：1 mg/ml (Please refer to the vial label for the specific concentration.)

保存溫度：Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4oC. For long-term storage, aliquot and store at -20oC or below. Avoid multiple freeze-thaw cycles.

運(yùn)輸溫度：4°C

預(yù)期分子量：36 kDa

產(chǎn)品形式：Liquid

存儲溶液：PBS, no preservative.

驗(yàn)證數(shù)據(jù)

WB Image

GAPDH antibody [GT239] detects GAPDH protein by western blot analysis.

A. 30 μg yeast whole cell lysate/extract

10% SDS-PAGE

GAPDH antibody [GT239] (GTX627408) dilution: 1:10000

The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

GTX627408(1).png

ICC/IF：

GAPDH antibody [GT239] detects GAPDH protein at cytoplasm by immunofluorescent analysis.

Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.

Green: GAPDH stained by GAPDH antibody [GT239] (GTX627408) diluted at 1:500.

Blue: Hoechst 33342 staining.

GTX627408(2).png

IHC-P：

GAPDH antibody [GT239] detects GAPDH protein at cytoplasm by immunohistochemical analysis.

Sample: Paraffin-embedded mouse kidney.

GAPDH stained by GAPDH antibody [GT239] (GTX627408) diluted at 1:200.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

產(chǎn)品部分引用文獻(xiàn)

1.S Shivaprasad et al. Viruses 2024; 16 (5) : 725 CRISPR Screen Reveals PACT as a Pro-Viral Factor for Dengue Viral Replication.

2.X Chen et al. Nature 2024; 628 (8009) : 818-825 Antisense oligonucleotide therapeutic approach for Timothy syndrome.

3.CP Chen et al. Heliyon 2024; 10 (7) : 29055 Synergistic effects of the combination of trametinib and alpelisib in anaplastic thyroid cancer with BRAF and PI3KCA co-mutations.

4.YL Chen et al. Viruses 2024; 16 : 523 Novel Anti-Viral Properties of the Herbal Extract of Davallia mariesii against Influenza A Virus.

訂購詳情

貨號	產(chǎn)品名稱	規(guī)格
GTX627408-S	GAPDH antibody	25 μl
GTX627408	GAPDH antibody	100 μl

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GeneTex熱銷抗體 — GAPDH抗體原理/介紹

WB Image

ICC/IF：

IHC-P：

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