花青-3- dUTP [Cy 3-dUTP] ：DNA標(biāo)記與檢測(cè)的高效工具

染料修飾的脫氧尿苷5'-三磷酸鹽（如氨基烯丙基dUTP）可用于通過常規(guī)酶摻入方法（如逆轉(zhuǎn)錄、缺口翻譯、隨機(jī)引物標(biāo)記或PCR）生產(chǎn)含染料的DNA。這種酶熒光標(biāo)記方法廣泛用于FISH探針和基于微陣列的實(shí)驗(yàn)。這種Cy3-dUTP綴合物可以用Spectrum Orange™濾光片組作為紅色熒光色（Spectrum Oranges™是Vysis的商標(biāo)）。它在功能上與Cyanine 3-dUTP（Perkin-Elmer的NEL578001EA）相當(dāng)。

艾美捷花青-3- dUTP [Cy 3-dUTP] ：

貨號(hào) AAT-17025

單位大?。?span style="font-family: Calibri;">25納摩爾

分子量：1150.00

溶劑：水

預(yù)期用途 僅供研究使用（RUO）

儲(chǔ)存條件：冷凍（<-15°C）；盡量減少光照

校正因子（260納米）：0.07

校正因子（280納米）：0.073

消光系數(shù)（cm -1 M -1）：150000

激發(fā)（納米）：555

發(fā)射（納米）：569

量子產(chǎn)率：0.15

圖：Cy3和iFluor®555 dUTP標(biāo)記的端粒探針在中期HeLa細(xì)胞中的熒光原位雜交。

花青-3- dUTP [Cy 3-dUTP] 文獻(xiàn)參考：

A Fluorescent DNA Hydrogel Aptasensor Based on the Self-Assembly of Rolling Circle Amplification Products for Sensitive Detection of Ochratoxin A.

Authors: Hao, Liling and Wang, Wei and Shen, Xueqing and Wang, Shuliu and Li, Qian and An, Faliang and Wu, Shijia

Journal: Journal of agricultural and food chemistry (2020): 369-375

Engineering nicking enzymes that preferentially nick 5-methylcytosine-modified DNA.

Authors: Gutjahr, Alice and Xu, Shuang-yong

Journal: Nucleic acids research (2014): e77

Digital detection of multiple minority mutants in stool DNA for noninvasive colorectal cancer diagnosis.

Authors: Deng, Lili and Qi, Zongtai and Zou, Binjie and Wu, Haiping and Huang, Huan and Kajiyama, Tomoharu and Kambara, Hideki and Zhou, Guohua

Journal: Analytical chemistry (2012): 5645-52

Digital analysis of the expression levels of multiple colorectal cancer-related genes by multiplexed digital-PCR coupled with hydrogel bead-array.

Authors: Qi, Zongtai and Ma, Yinjiao and Deng, Lili and Wu, Haiping and Zhou, Guohua and Kajiyama, Tomoharu and Kambara, Hideki

Journal: The Analyst (2011): 2252-9

Microisolation and microcloning of bovine X-chromosomes for identification of sorted buffalo (Bubalus bubalis) spermatozoa.

Authors: Zhuang, Xin-jie and Lu, Yang-qing and Zhang, Ming and Lu, Sheng-sheng and Lu, Ke-huan

Journal: Animal reproduction science (2011): 32-6