Overview

Human embryonic stem (ES) cells are routinely passaged as clumps, rather than single cells. To generate clumps appropriate for passaging, both enzymatic and mechanical (by dissection or scraping) methods can be used. Collagenase Type IV is an enzyme that is routinely used at 1 mg/mL for the generation of human ES cell and iPS cell clumps for passaging.

Contains:

• 1 mg/mL Collagenase Type IV isolated from Clostridium histolyticum 
• DMEM/F-12

Subtype:

酶

Species:

人; 小鼠; 非人靈長類動物; 其他; 大鼠