72302 stemcell 72302 Y-27632 （Dihydrochloride）

Description

Y-27632 is a cell-permeable, highly potent and selective inhibitor of Rho-associated, coiled-coil containing protein kinase (ROCK). Y-27632 inhibits both ROCKI (K_i = 220 nM) and ROCKII (K_i = 300 nM) by competing with ATP for binding to the catalytic site. (Davies et al., Ishizaki et al.)

Published Applications

MAINTENANCE:

• Enhances survival of human embryonic stem (ES) cells when they are dissociated to single cells by preventing dissociation-induced apoptosis (anoikis), thus increasing their cloning efficiency. (Watanabe et al.)
• Improves embryoid body formation using forced-aggregation protocols. (Ungrin et al.)
• Increases the survival of cryopreserved single human ES cells after thawing. (Li et al.)

REPROGRAMMING

• Direct lineage reprogramming of fibroblasts to mature neurons, in combination with CHIR99021, Valproic Acid, RepSox, Forskolin, SP600125, and Gö6983 (Hu et al.).