excellness Prime excellness代理,PrimeCoat彈性基底培養(yǎng)皿板

干細(xì)胞不同基底硬度機(jī)械力培養(yǎng)系統(tǒng)

特別適合細(xì)胞、干細(xì)胞不同基底硬度牽張拉伸刺激培養(yǎng)或靜態(tài)培養(yǎng)（模量剛度范圍1-80 kPa）。

美國(guó)flexcell提供CellSoft™型號(hào)的不同基底硬度培養(yǎng)耗材包括：不同基底硬度牽張拉伸培養(yǎng)板、培養(yǎng)皿和可用于顯微觀察的腔室載玻片（圓形多孔板）多種不同的種類，該cellsoft不同基底硬度培養(yǎng)耗材共價(jià)包被Collagen I或其他蛋白配合flexcell細(xì)胞牽張拉伸培養(yǎng)儀，可對(duì)細(xì)胞進(jìn)行靜態(tài)或動(dòng)態(tài)牽拉應(yīng)力刺激。更重要的一點(diǎn)，新型的CellSoft™ 培養(yǎng)板可以反復(fù)胰酶消化和再接種細(xì)胞，蛋白包被的表面可以重復(fù)使用多達(dá)三次。

美國(guó)Flexcell公司專注于細(xì)胞組織應(yīng)力（牽張拉伸應(yīng)力、三維水凝膠牽張拉伸應(yīng)力、壓應(yīng)力和流體切應(yīng)力等）加載刺激培養(yǎng)產(chǎn)品的設(shè)計(jì)和制造，提供*的體外細(xì)胞拉應(yīng)力、壓應(yīng)力和流體剪切應(yīng)力加載刺激與立體水凝膠支架三維細(xì)胞組織牽拉加載培養(yǎng)系統(tǒng)而*。

其產(chǎn)品成熟度高、成功應(yīng)用文獻(xiàn)量達(dá)4000多篇，國(guó)內(nèi)有包括上海交通大學(xué)、復(fù)旦大學(xué)、同濟(jì)大學(xué)、上海第九醫(yī)院、中科院力學(xué)所、北京大學(xué)第三醫(yī)院、北航生物與醫(yī)學(xué)工程學(xué)院、都醫(yī)科大學(xué)、廣州醫(yī)科大學(xué)、南方科技大學(xué)、福建協(xié)和醫(yī)院、南方醫(yī)科大學(xué)近100家成功高校、醫(yī)院及基礎(chǔ)科研單位使用，無技術(shù)風(fēng)險(xiǎn)和使用風(fēng)險(xiǎn)，flexcell體外高通量細(xì)胞牽張拉伸力、壓應(yīng)力以及流體剪切力加載培養(yǎng)系統(tǒng)已成為細(xì)胞力學(xué)體外加載模型的黃金標(biāo)準(zhǔn)，是細(xì)胞組織力學(xué)研究者的shou選。

FX-5000T細(xì)胞牽張拉伸應(yīng)力加載系統(tǒng)(Flexcell FX5000 Tension system)

系統(tǒng)基本原理（負(fù)氣壓交換模式）：

橡膠密封墊在細(xì)胞培養(yǎng)板基底膜與基板之間形成封閉腔，把此密封腔的進(jìn)、出氣管插入二氧化碳培養(yǎng)箱里，把此密封腔放入二氧化碳培養(yǎng)箱， 利用封閉腔抽真空產(chǎn)生的負(fù)壓使彈性基底膜（拉動(dòng)三維支架）發(fā)生形變，通過計(jì)算機(jī)控制系統(tǒng)調(diào)節(jié)氣體的壓力來改變基底膜的形變量,進(jìn)而使貼壁生長(zhǎng)的細(xì)胞受到牽拉加載刺激。

亮點(diǎn)：
1）該系統(tǒng)對(duì)二維、三維細(xì)胞和組織各種培養(yǎng)物提供軸向和圓周應(yīng)力加載；不但具有雙軸向拉伸力加載，還具備單軸向加力功能
2）計(jì)算機(jī)控制的應(yīng)力加載系統(tǒng)，為體外培育的細(xì)胞提供的、可控制的、可重復(fù)的、靜態(tài)的或者周期性的應(yīng)力變化。
3）使用真空泵，抻拉培養(yǎng)板底部的彈性硅膠模，細(xì)胞培養(yǎng)板底部伸展度可達(dá)到33%，通過氣體裝置可以自動(dòng)調(diào)節(jié)和控制應(yīng)力。
4）基于柔性膜基底變形、受力均勻;
5）可實(shí)時(shí)觀察細(xì)胞、組織在應(yīng)力作用下的反應(yīng);
6）*的flexstop隔離閥可使同一塊培養(yǎng)板力的一部分培養(yǎng)孔的細(xì)胞受力，一部分培養(yǎng)孔的細(xì)胞不受力，方便對(duì)比實(shí)驗(yàn);
7）與壓力傳導(dǎo)儀整合，同時(shí)兼?zhèn)涠嗤ǖ兰?xì)胞壓力加載功能;
8）與Flex Flow平行板流室配套，可在牽拉細(xì)胞的同時(shí)施加流體切應(yīng)力;
9）多達(dá)4通道，可4個(gè)不同程序同時(shí)運(yùn)行，進(jìn)行多個(gè)不同拉伸形變率對(duì)比實(shí)驗(yàn)；
10）同一程序中可以運(yùn)行多種頻率，多種振幅和多種波形；
11）加載模擬波形種類豐富：靜態(tài)波形、正旋波形、心動(dòng)波形、三角波形、矩形以及各種特制波形；
12）更好地控制在超低或超高應(yīng)力下的波形；
13)電腦系統(tǒng)對(duì)牽張拉伸力加載周期、大小、頻率、持續(xù)時(shí)間智能調(diào)控
14)加載分析各種細(xì)胞在牽張拉應(yīng)力刺激下的生物化學(xué)反應(yīng)
15)伸展度范圍廣：0-33%
16)牽拉頻率范圍廣：0.01-5Hz

17)典型應(yīng)用:

該系統(tǒng)感應(yīng)各種細(xì)胞在應(yīng)力刺激下的生物化學(xué)反應(yīng)，例如：骨骼細(xì)胞，肺細(xì)胞，心肌細(xì)胞，血細(xì)胞，皮膚細(xì)胞，
肌腱細(xì)胞，韌帶細(xì)胞，軟骨細(xì)胞和骨細(xì)胞等各種2D或3D細(xì)胞組織。
典型應(yīng)用科室:

2、FX-5000C細(xì)胞壓力加載系統(tǒng)(flexcell FX5000 Compression system)——提供樣機(jī)體驗(yàn)

系統(tǒng)基本原理（正氣壓交換模式）：

利用橡膠密封墊在細(xì)胞培養(yǎng)板基底膜與基座之間形成封閉腔，把此密封腔的進(jìn)、出氣管插入二氧化碳培養(yǎng)箱里，把此密封腔放入二氧化碳培養(yǎng)箱，利用封閉腔正氣壓擠壓培養(yǎng)孔里的活塞，進(jìn)而使活塞和固定臺(tái)之間的凝膠三維培養(yǎng)物間接受到壓力發(fā)生形變，通過計(jì)算機(jī)控制系統(tǒng)調(diào)節(jié)氣體的壓力來改變基底膜的形變量。

(注釋：壓力加載培養(yǎng)板每個(gè)培養(yǎng)孔里都有一對(duì)活塞或固定臺(tái))

亮點(diǎn)

1）該系統(tǒng)對(duì)各種組織、三維細(xì)胞培養(yǎng)物提供周期性或靜態(tài)的壓力加載；
2）基于柔性膜基底變形、受力均勻;
3）可實(shí)時(shí)觀察細(xì)胞、組織在壓力作用下的反應(yīng);
4）可有選擇性地封阻對(duì)細(xì)胞的應(yīng)力加載;

5）同時(shí)兼?zhèn)涠嗤ǖ兰?xì)胞牽拉力加載功能;

6）多達(dá)4通道，可4個(gè)不同程序同時(shí)運(yùn)行，進(jìn)行多個(gè)不同壓力形變率對(duì)比實(shí)驗(yàn)；

7）同一程序中可以運(yùn)行多種頻率(0.01- 5 Hz)，多種振幅和多種波形；
8）更好地控制在超低或超高應(yīng)力下的波形；
9）多種波形種類：靜態(tài)波形、正旋波形、心動(dòng)波形、三角波形、矩形以及各種特制波形；
10)電腦系統(tǒng)對(duì)壓力加載周期、大小、頻率、持續(xù)時(shí)間智能調(diào)控
11)壓力范圍：0.1 - 14磅，夾在活塞和固定臺(tái)之間的BioPress細(xì)胞培養(yǎng)板可承受正壓力0.1---14磅
12)典型應(yīng)用科室: 
檢測(cè)各種三維細(xì)胞組織在壓力作用下的生物變化、反應(yīng)， 
例如：軟骨組織，椎間盤骨組織，肌腱組織，韌帶組織，以及從肌肉，肺，心臟，血管，皮膚，肌腱，韌帶，軟骨和骨中分離出來的細(xì)胞。 
13)在智能電腦主機(jī)的控制下，壓力傳導(dǎo)儀內(nèi)的密封閥門裝置自動(dòng)調(diào)節(jié)和控制壓力。 
14)系統(tǒng)具有模塊化易升級(jí)，可擴(kuò)展拉應(yīng)力加載、流利切應(yīng)力加載、三維細(xì)胞組織培養(yǎng)功能。具有細(xì)胞組織力學(xué)所要求的所有類型：牽張拉伸力、壓力、流體切應(yīng)力加載刺激功能。 
15)通過StagePress顯微壓應(yīng)力加載設(shè)備,實(shí)時(shí)觀察細(xì)胞、組織在拉/壓應(yīng)力作用下的反應(yīng) 

3、全自動(dòng)可牽張拉伸刺激立體水凝膠支架三維細(xì)胞培養(yǎng)系統(tǒng)(Flexcell TissueTrain System)——提供樣機(jī)體驗(yàn)

二、excellness代理,PrimeCoat彈性基底培養(yǎng)皿板

PrimeCoat series
 

The ExCellness PrimeCoat series is designed specifically to provide a biomimetic cell culture environment that improves cell characteristics and phenotype in laboratory applications.

Key features

• PrimeCoat elastic substrates are easy to use for cell culture and subsequent analysis.
• PrimeCoat elastic substrates are available with 6 degrees of softness within the elasticity range of body tissues: 2, 5, 10, 15, 30 and 100 kPa.
• PrimeCoat elastic substrates are available in 4 standard formats: 100 mm diameter dishes, 40 mm diameter dishes, 24-well plates and 20x20 mm cover slips.
• PrimeCoat elastic substrates require to be coated by the end user to promote cell adhesion.
• PrimeCoat elastic substrates are transparent. Cells can be visualized with standard transmission light microscopes (e.g., Phase contrast, DIC).
• PrimeCoat elastic substrates are compatible with most standard molecular or cellular techniques: immunofluorescence, immunohistochemistry, protein analysis (e.g., Western blotting, and RNA/DNA extraction).

Biomimetic cell culture substrates at reach

PrimeCoat series combines simplicity and accessibility:

• simplicity of cell handling
• straightforward compatibility with cell analysis tools
• accessible prices

ExCellness in selected peer reviewed publications

In the following peer-reviewed publications, ExCellness biomimetic cell culture devices have been used or cited:

• A Glentis et al. Cancer-associated fibroblasts induce metalloprotease-independent cancer cell invasion of the basement membrane. Nat Commun. 2017 Oct 13;8(1):924.
• N Vedrenne et al. Isolation of astrocytes displaying myofibroblast properties and present in multiple sclerosis lesions. Neurochem. Res. 2017 Apr 22. 
• X L Chen et al. MicroRNA-21 preserves the fibrotic mechanical memory of mesenchymal stem cells. Nature Materials 16, 379–389 (2017).
• MR Zeglinski et al. Chronic expression of Ski induces apoptosis and represses autophagy in cardiac myofibroblasts. Biochim.  Biophys. Acta. 2016 Jun;1863(6 Pt A):1261-8.
• H Chen et al. Mechanosensing by the α6-integrin confers an invasive fibroblast phenotype and mediates lung fibrosis. Nat. Commun. 2016 Aug 18;7: 12564.
• NP Talele et al. Expression of α-Smooth Muscle Actin Determines the Fate of Mesenchymal Stromal Cells. Stem Cell Reports. 2015 Jun 9;4(6) : 1016-30.

• V Sarrazy et al. Integrins avb5 and avb3 promote latent TGF-beta 1 activation by human cardiac fibroblast contraction. Cardiovasc Res (2014) 102 (3)
• VF Achterberg et al. The nano-scale mechanical properties of the extracellular matrix regulate dermal fibroblast function.J Invest Dermatol. 2014 Jul;134(7):1862-72.
• JA Cadby et al. Differences between the Cell Populations from the Peritenon and the Tendon Core with Regard to Their Potential Implication in Tendon Repair. 2014. PLoS ONE 9(3): e92474.
• T. Grand et al. Aggravation of Cardiac Myofibroblast Arrhythmogeneicity by Mechanical Stress. Cardiovasc Res. 2014 Dec 1;104(3):489-500.
• JA Cadby. Can we improve tendon healing in the horse? A multi-angle study of a multi-facet problem. ISBN: 978-90-5335-715-6.
• A Vashist et al. Recent advances in hydrogel based drug delivery systems for the human body. J. Mater. Chem. B, 2014,2, 147-166.
• EP van der Veer et al. The RNA-Binding Protein Quaking is a Critical Regulator of Vascular Smooth Muscle Cell Phenotype. Circ Res. 2013 Oct 12;113(9):1065-75.
• A De Boeck et al. Differential secretome analysis of cancer-associated fibrobroblasts and bone marrow-derived precursors toidentify microenvironmental regulators of colon cancerprogression. Proteomics 2013,13,379-388.
• C Godbout et al. The Mechanical Environment Modulates Intracellular Calcium Oscillation Activities of Myofibroblasts.PLoS One. 2013; 8(5): e64560.
• S Constant et al. Colon Cancer: Current Treatments and Preclinical Models for the Discovery and Development of New Therapies. Drug discovery; Editor Hany A. El-Shemy. ISBN 978-953-51-0906-8.
• JL Balestrini et al. The mechanical memory of lung myofibroblasts. Integr. Biol., 2012,4, 410-421.
• Stem Cells and Cancer Stem Cells, Volume 8. Therapeutic Applications in Disease and Injury. Editors M.A. Hayat. ISBN 978-94-007-4797-5.

• A Skardal et al. Bioprinted amniotic fluid-derived stem cells accelerate healing of large skin wounds. Stem Cells Transl Med. 2012 Nov;1(11):792-802. doi: 10.5966/sctm.2012-0088. Epub 2012 Oct 29.
• A Skardal et al. Substrate elasticity controls cell proliferation, surface marker expression and motile phenotype in amniotic fluid-derived stem cells. J Mech Behav Biomed Mater. 2013 January; 17: 307-316.
• BJ Crider et al. Myocardin-Related Transcription Factors A and B Are Key Regulators of TGF-b1-Induced Fibroblast to Myofibroblast Differentiation. Journal of Investigative Dermatology (2011) 131, 2378-2385.
• L Follonier Casla et al. A new lock-step mechanism of matrix remodelling based on subcellular contractile events. Journal of Cell Science 123, 1751-1760.

訂貨信息：

Dishes
Product Code     Description     
01. 100.002.00     2 kPa culture surface 100 mm ø     
01. 100.005.00     5 kPa culture surface 100 mm ø     
01. 100.010.00     10 kPa culture surface 100 mm ø     
01. 100.015.00     15 kPa culture surface 100 mm ø     
01. 100.030.00     30 kPa culture surface 100 mm ø     
01. 100.100.00     100 kPa culture surface 100 mm ø     

Product Code     Description     
01. 035.002.00     2 kPa culture surface 35 mm ø     
01. 035.005.00     5 kPa culture surface 35 mm ø     
01. 035.010.00     10 kPa culture surface 35 mm ø     
01. 035.015.00     15 kPa culture surface 35 mm ø     
01. 035.030.00     30 kPa culture surface 35 mm ø     
01. 035.100.00            100 kPa culture surface 35 mm ø 
 
Multi-well plates 

Product Code     Description     
04.024.002.00     2 kPa culture surface 24-well plate     
04.024.005.00     5 kPa culture surface 24-well plate     
04.024.010.00     10 kPa culture surface 24-well plate     
04.024.015.00     15 kPa culture surface 24-well plate     
04.024.030.00     30 kPa culture surface 24-well plate     
04.024.100.00     100 kPa culture surface 24-well plate 

    
Cover slips

Product Code     Description 

03.020.002.00     2 kPa culture surface 20x20 mm c.slip 
03.020.005.00     5 kPa culture surface 20x20 mm c.slip 
03.020.010.00     10 kPa culture surface 20x20 mm c.slip 
03.020.015.00     15 kPa culture surface 20x20 mm c.slip 
03.020.030.00     30 kPa culture surface 20x20 mm c.slip