T040-1.0，0.2 Biontex哺乳動物細(xì)胞轉(zhuǎn)染試劑

METAFECTENE ®PRO(專業(yè)級)是針對新標(biāo)準(zhǔn)的哺乳動物細(xì)胞轉(zhuǎn)染的一代轉(zhuǎn)染試劑。它成分新穎，結(jié)合了Biontex的“RMA"技術(shù)和新型毒性優(yōu)化模塊。

優(yōu)點： 改善毒理學(xué)（毒性優(yōu)化技術(shù)）轉(zhuǎn)染到每個細(xì)胞的高表達率大量用戶的報告綜合庫尤其適用于桿狀病毒質(zhì)粒的轉(zhuǎn)染 METAFECTENE PRO是針對新標(biāo)準(zhǔn)的哺乳動物細(xì)胞轉(zhuǎn)染的一代轉(zhuǎn)染試劑。采用結(jié)合毒性優(yōu)化（TOP）技術(shù)，Biontex成功地在顯著提高RMA功能促進DNA釋放進入細(xì)胞的效率的同時zui小化了對細(xì)胞的毒性。除了能加強細(xì)胞的轉(zhuǎn)染效率之外，METAFECTENE PRO的另一主要特點是潛在提高每個細(xì)胞的基因表達。 TOP技術(shù)zui小化毒性是現(xiàn)代轉(zhuǎn)染試劑達到率和廣泛應(yīng)用的首要前提條件。 TOP技術(shù)的基本準(zhǔn)則是以通過模擬自然結(jié)構(gòu)原件加強生物降解能力、保證顯著提高細(xì)胞耐受性為目標(biāo)。另外，它的物理化學(xué)修飾會降低脂質(zhì)復(fù)合體的穩(wěn)定性從而促進DNA在細(xì)胞質(zhì)的釋放。

產(chǎn)品規(guī)格

應(yīng)用 核酸轉(zhuǎn)染真核細(xì)胞

制定 陽離子和中性脂質(zhì)融于水中

檢測 每1ml試劑可使用1500次/六孔板、400次/60mm培養(yǎng)皿

運輸 常溫

儲存 ≤ –150 C

保質(zhì)期 一年

目錄號

產(chǎn)品 貨號 規(guī)格 目錄價

METAFECTENERPRO T040-0.2 0.2 ml 720

METAFECTENERPRO T040-1.0 1.0 ml 3500

METAFECTENERPRO T040-2.0 2 ×1.0 ml 6400

METAFECTENERPRO T040-5.0 5× 1.0 ml 12000

應(yīng)用的細(xì)胞類型 Application Notes from independent scientists siRNA transfection Cell Type Description Growth Properties Cat. No. Notes 1BR3 Human primary fibroblasts adherent HEK293 Human embryonic kidney adherent HeLa Human cervix carcinoma adherent ATCC CCL-2 LN-308 Human glioma adherent T98G Human glioma adherent ATCC U87MG Human glioma adherent UACC-257 Human Melanoma adherent Plasmid transfection （Cell lines） Cell Type Description Growth Properties Cat. No. Notes 1BRneo Transformed human fibroblasts adherent 55.1 Immortalized human cord blood suspension 55.2 Immortalized human cord blood suspension 143B Human Osteosarcoma adherent 293T Human kidney cells, SV40 large T antigen inserted adherent ATCC A172 Human glioma adherent A375 Human Melanoma adherent A549 NSCLC Human lung adenocarcinoma adherent alphaTC1 Mouse pancreatic insulinoma adherent B16-F10 Mouse melanoma adherent BE（2）M17 Human neuroblastoma adherent betaTC3 Mouse pancreatic insulinoma adherent BHK-21 Baby hamster kidney adherent C2C12 Mouse myoblast adherent ATCC CRL-1772 C6 Rat glioma adherent CHO Chinese hamster ovary adherent CHO-K1 Chinese hamster ovary adherent ATCC Colo205 Human colon carcinoma adherent ATCC COS-7 African green monkey kidney suspension ATCC COS-7 African green monkey kidney adherent ATCC E14/T Mouse embryonic stem adherent EVLC2 Human umbilical vein endothelia adherent HaCaT Human immortalized keratinocytes adherent HAEC human aortic endothelial adherent HCT116 Human colon carcinoma adherent ATCC HEK293 Human embryonic kidney suspension ATCC HEK293 Human embryonic kidney adherent ATCC HeLa Human cervix carcinoma adherent ATCC CCL-2 HepG2 Human hepatoma adherent Hep 3B Human hepatocellular carcinoma adherent HT-1080 Human breast fibrocarcinoma adherent ATCC IMR32 Human Neuroblastoma adherent Jurkat Human T lymphoblastic leukeamia suspension Kelly Human neuroblastoma adherent KG1 Human myelogenous leukemia suspension ATCC La1-5S Human neuroblastoma adherent LS174T Human colon adenocarcinoma adherent ATCC LN-308 Human glioma adherent LNT-229 Human glioma adherent M2-10B4 Murine bone marrow stroma adherent MCF-7 Human caucasian breast adenocarcinoma adherent ATCC MDA-MB231 Human breast carcinoma adherent MDCK Madin Darby Canine Kidney adherent MEF Mouse embryonic fibroblast adherent MG-63 Human osteosarcoma adherent MIN6 pancreatic beta-cells adherent MLEC32 Mink epithelial lung adherent mPAC Mouse pancreatic insulinoma adherent mpkCCD Mouse principal kidney cortical collecting duct adherent N18TG2 Mouse neuroblastoma adherent N2a Mouse Neuroblastoma adherent N-Tera2 Human teratocarcinoma adherent NG 108-15 Neuroblastoma X glioma hybrid adherent ATCC NIH-3T3 Mouse fibroblast adherent UCSF OLN93 Rat oligodendroglial adherent P388 45R Mus musculus （mouse） suspension Panc-1 Human exocrine pancreas carcinoma adherent ATCC PC 12 Neuroendocrine cell adherent PC 12 Neuroendocrine cell suspension Phi-NX Originally based on 293T adherent Raji Human B lymphocytes suspension RAW264.7 Murine macrophage adherent RD Rabdomiosarcoma adherent RF24 Human vascular umbilical endothelia adherent RGE rat glomerular endothelial adherent S2 Drosophila adherent S91 Mouse melanoma adherent SAOS-2 Human Osteosarcoma adherent SCCVII Murine squamous carcinoma adherent SH-SY5Y Human neuroblastoma adherent SMA-560 Mouse glioma adherent SK-N-BE Human neuroblastoma adherent ATCC SK-N-SH Human neuroblastoma adherent SK-UT-1 Human mesoderam tumor adherent SW480 Human colon adenocarcinoma adherent ATCC SW982 Human sarcoma adherent T98G Human glioma adherent ATCC U-2 OS Human osteosarcoma adherent U87MG Human glioma adherent