3D細(xì)胞共培養(yǎng)系統(tǒng)-3D CoSeedis，

模塊化多功能3D細(xì)胞共培養(yǎng)系統(tǒng)

在3D共培養(yǎng)新標(biāo)準(zhǔn)

3D CoSeedis™是一種新型的無支架3D細(xì)胞共培養(yǎng)系統(tǒng)。 它由*的基于瓊脂糖的基質(zhì)組成，其中包含一系列圓錐形微孔。 這種布置的特殊且專有的形貌允許以高度可重復(fù)且一致的方式形成球形和非球形細(xì)胞聚集體。 陣列內(nèi)的圓錐形微孔可確定聚集體的體積，從而確定細(xì)胞的生長。 此外，3D CoSeedis™系統(tǒng)的模塊化組成允許與實際測試細(xì)胞物理分離的飼養(yǎng)細(xì)胞共培養(yǎng)（遠(yuǎn)距離共培養(yǎng)）。 因此，此設(shè)置負(fù)責(zé)定義條件下的協(xié)議標(biāo)準(zhǔn)化。

3D CoSeedis™矩陣的主要功能，模塊化設(shè)置

與飼養(yǎng)細(xì)胞接觸或遠(yuǎn)距離共培養(yǎng)
●多組合3種細(xì)胞類型以分析相互作用
●可能出現(xiàn)血清，血清減少或無血清的情況
●組織特定的ECM組件的應(yīng)用

 基于瓊脂糖的滲透性基質(zhì)，允許細(xì)胞聚集體的長期3D培養(yǎng)

●充足的營養(yǎng)，氧氣和分解代謝物–有效處置有毒廢物
●有效利用物理分離的飼養(yǎng)細(xì)胞（遠(yuǎn)距離共培養(yǎng)）
 ●在標(biāo)準(zhǔn)或定義的媒體中進(jìn)行培養(yǎng)

多功能適用性，適用于各種細(xì)胞
●支持球狀和非球狀聚集體的形成

●適用于高通量和高含量的應(yīng)用

流程指導(dǎo)的工作流程協(xié)議
經(jīng)過驗證的協(xié)議●標(biāo)準(zhǔn)化和優(yōu)化的組件●高度可重復(fù)的3D細(xì)胞構(gòu)造●適用于高通量和高含量的應(yīng)用

 適用的細(xì)胞系統(tǒng)我們的學(xué)術(shù)合作伙伴已成功測試了這些細(xì)胞的聚集球體形成。 在出版物中描述了詳細(xì)信息：用于獨(dú)立于粘附的三維細(xì)胞培養(yǎng)和動態(tài)體積測量的深錐形瓊脂糖微孔陣列Andreas R. Thomsen等，Lab Chip，DOI 10.1039 / C7LC00832E。

3D類器官系統(tǒng)的標(biāo)準(zhǔn)化協(xié)議
3D CoSeedis™系統(tǒng)的模塊化組成允許進(jìn)行遠(yuǎn)程共培養(yǎng)，因此可以實現(xiàn)協(xié)議的標(biāo)準(zhǔn)化。 abc biopply的產(chǎn)品組合已包含適用于多種共培養(yǎng)的標(biāo)準(zhǔn)化協(xié)議，尤其是用于培養(yǎng)不同腫瘤的協(xié)議

High-Throughput / High-Content Screening Applications

3D CoSeedis™ is an ideally suited production tool to grow spheroidal 3D cell constructs in large scale, particularly for the use in HTS/HCS applications. With the various formats (2x2, 1x1, 0.5x0.5), it is possible to grow up to 1’200, 5’280, and 16’320 spheroids per plate, respectively.

The major advantages for subsequent HTS/HCS are:

• 3D CoSeedis™ matrix consists of agarose => biologically inert carrier matrix
• Substantial reduction of cell culture work (50’000 3D constructs/day ® 3 – 10  3D CoSeedis™ plates).
  > 5’000 3D constructs per 6-well plate; >16’000 per 24-well plate.
• Highly reproducible and homogeneous 3D cell aggregates across matrix and plates.
• Increased screening efficiency through highly homogenous 384-well plates seeded with 1 to several 3D constructs / well.
• Long-term studies possible: up to 70 days in culture.
• Dual and sequential treatments possible over extended
  period of time with lots of up to 900 cell aggregates.
• Fully validated for a wide range of tumour 3D aggregates.
• Modular set-up (e.g. distance co-cultures) significantly extends
  the range of testable cells in 3D including primary cells and iPS.
• Fully validated read-outs:
  • Growth monitoring
  • Viability
  • Cytotoxicity
  • Histology: paraffin and frozen
  • Colony forming assay

3D CoSeedis in High-Throughput / High-Content Screening

Research Applications

3D CoSeedis™ has been validated for various applications and according protocols have been developed. The different application notes can be downloaded here or contact us for further information.

Colony Forming Assay

A new approach to the colony forming assay in cancer research. 3D CoSeedis™ simplifies and facilitates the set-up, read-out and analysis of the assay substantially.

Download Application Note

For further details you may also refer to the following Whitepaper.

Viability / Cytotoxicity

The application note addresses viability and/or cytotoxicity of 3D cell constructs grown in 3D CoSeedis™. The protocol describes an extension to all existing 3D CoSeedis™ systems and can be implemented easily without further requirements in equipment or appliances.

Cryo-embedding

3D CoSeedis™ offers an easy and elegant way to embed 3D constructs for cryo-sectioning without the need to manipulate individual spheroids. It reduces tricky and cumbersome manipulations and prevents damage or loss of 3D constructs.

References

• Tspan8 is expressed in breast cancer and regulates E-cadherin / catenin signalling and metastasis accompanied by increased circulating extracellular vesicles
  Maren Vogelstaetter et al., J. Pathol., 2019, DOI:10.1002/path.5281

• 3D Cellular Architecture Affects MicroRNA and Protein Cargo of Extracellular Vesicles
  Sara Rocha et al., Adv. Sci. 2018, 1800948; DOI: 10.1002/advs.201800948

• A deep conical agarose microwell array for adhesion independent three-dimensional cell culture and dynamic volume measurement
  Andreas R. Thomsen et al., Lab Chip, 2018, 18, 179; DOI: 10.1039/c7lc00832e
• Proteome Profiling of Primary Pancreatic Ductal Adenocarcinomas Undergoing Additive Chemoradiation Link ALDH1A1 to Early Local Recurrence and Chemoradiation Resistance
  V. O. Oria et al., Translational Oncology, Vol. 11, Issue 6, Dec. 2018; DOI.org/10.1016/j.tranon.2018.08.001

• A binary approach to the colony forming assay: reliable and reproducible read-outs using 3D CoSeedis™