Invitrogen 18374058 10次- 5´RACE System for Rapid Amplification of cDNA Ends

5’Race System

品名:  5´ RACE System for Rapid Amplification of cDNA Ends, Version 2.0

Cat. No. 18374-058

Product Size: 10 reactions

產(chǎn)品介紹:

The 5´ RACE System, Version 2.0 is suitable for rapid amplification of cDNA ends （RACE） （1） and anchored PCR （2） between a defined point in the mRNA and the 5´ end. The system is useful for the amplification of rare messages for which little sequence information may be available, and for capturing the 5´ end information of mRNA （3,4）. The 5´ RACE system:

Uses the advanced capabilities of SuperScript® II RNase H- RT for greater first-strand cDNA yields and increased cDNA lengths

Prepares specific cDNA from up to 1 µg of total or poly（A）+ RNA in one reaction

Offers improved first-strand cDNA purification procedure that yields cDNA suitable for tailing

Uses recombinant TdT with optimized reaction conditions in a PCR-compatible buffer to provide an efficient primer annealing site for PCR （Figure 1）

Contents & Storage:

The 5´ RACE System is supplied with the components listed in Table 1. Store the reagents at -20°C and the DNA purification components at +4°C. All reagents guaranteed stable for 6 months when properly stored.