Invitrogen 12578035- GB C-TERM GATEWAY IN VITRO KIT

The GeneBLAzer™ C-terminal Gateway® Fusion Kit contains the Gateway®-adapted destination
vector, pcDNA™6.2⁄cGeneBLAzer™-DEST, designed for use with the GeneBLAzer™ Technology. The
pcDNA™6.2⁄cGeneBLAzer™-DEST vector enables easy fusing of any gene to a mammalian-optimized b-lactamase gene, bla, and facilitates in vivo or in vitro detection of ß-lactamase reporter activity in mammalian cells using a
unique fluorescent substrate. Use of the GeneBLAzer™ Technology provides a
highly sensitive and accurate method to quantitate gene expression in
mammalian cells. The vector contains the following elements:
• Human cytomegalovirus immediate-early （CMV） promoter⁄enhancer for
high-level expression in a wide range of mammalian cells
• ß-lactamase bla（M） reporter gene for C-terminal fusion to the gene of interest
• Two recombination sites, attR1 and attR2, downstream of the CMV promoter
for recombinational cloning of the gene of interest from an entry clone
• Chloramphenicol resistance gene located between