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GE Pharmacia 17-0851-01 預(yù)裝脫鹽柱 Disposable PD-10 Desalting Columns

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PD-10

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         上海嘉適科學(xué)儀器有限公司成立于1996年,座落在浦東醫(yī)學(xué)園區(qū)內(nèi),是美國JUST scientific CO,LTD公司的中國分理處,慣程美國實驗室供應(yīng)商的標(biāo)準(zhǔn)為中國實驗室和企業(yè)提供優(yōu)良的實驗室裝備,just for you的理念不僅為美國東部大學(xué)提供大批高質(zhì)量、高性能的實驗室安全和檢測儀器,也為實驗室節(jié)省了選購儀器所花費(fèi)的人力、物力。性價比和優(yōu)良的服務(wù)我們一直會延伸到中國的每一個實驗室和客戶手中。

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預(yù)裝脫鹽柱 Disposable PD-10 Desalting Columns
GE預(yù)裝脫鹽柱 Disposable PD-10 Desalting Columns IMPROVED:一次性脫鹽緩沖液交換制備。 球蛋白分離范圍:1,000-5,000 柱體積:8.3ml

Technical Information


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PD-10 columns are packaged with a convenient column stand¾the PD-10 Desalting Workmate. Use LabMate Buffer Reservoir for easier equilibration.

 


Disposable PD-10 Desalting Columns offer an inexpensive and convenient alternative to lengthy and troublesome dialysis procedures. In minutes, biological samples are desalted and small molecular weight solutes are removed or exchanged with excellent recoveries of macromolecules.

 


The instructions for Disposable PD-10 Desalting Columns have been updated with two new application protocols: gravity or centrifugation, to increase the flexibility of the product. The gravity protocol allows a simple cleanup of samples with one or several columns in parallel, without the need for a purification system. The spin protocol enables runs in parallel in a standard centrifuge with minimal dilution of the eluted sample.

 

Four adapters are included in each product pack to enable easy centrifugation. Together with LabMate PD-10 Buffer Reservoir, wash and equilibration buffer can be applied in one step with the gravity flow protocol (order separay).

 


Empty PD-10 columns are recommended for use with bulk media for affordable and versatile sample preparation.

 

TECHNICAL SPECIFICATIONS
Disposable PD-10 Desalting Columns
Matrix   Sephadex G-25 Medium  
Separation mechanism   According to size  
Particle size range  85-260 µm  
Exclusion limit   Mr 5000  
Sample volume (gravity)   1.0 to 2.5 ml  
Sample volume (spin)   1.75 to 2.5 ml  
Column volume   13.5 ml  
Medium volume   8.3 ml  
Column material   Polypropylene barrel and polyethylene frits  
Chemical stability   All commonly used buffers  
Working pH range  2¾13  
Storage temperature  4°C to 30°C  

 

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Removal of NaCl from BSA using the gravity protocol. Recovery of the sample was 95% and the desalting capacity was > 99%.

 

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PD-10 Desalting Columns give fast desalting with gravity flow or spin protocols. The blue adapters are used together with the spin protocols.
 



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