070222h Human Interferon,Beta（IFNb） ELISA Kit

一、人β干擾素 ELISA試劑盒的產(chǎn)品簡述：

       ELISA 品  牌： USCN

       ELISA 貨  號：070222h

       ELISA英文名：human IFNb ELISAKit

       ELISA中文名：人β干擾素,IFNb ELISA 試劑盒

二、人IFNb ELISAKIT的技術(shù)參數(shù)：
      1. 檢測范圍：

      2. 靈  敏  度：

      3. 適用標本：

      4.保存條件：2-8℃

       5. 有 效  期： 6個月

三、human IFNb ELISA試劑盒的產(chǎn)品用途：
      產(chǎn)品用途：酶聯(lián)免疫實驗（ELISA實驗）

      備     注：僅供參考，請以隨貨紙質(zhì)說明書為準，更多產(chǎn)品如抗體（antibody）；蛋白（protein）；鱟試劑；內(nèi)毒素工作標準品；內(nèi)毒素指示劑；無熱原檢查用水；無熱原吸咀；紫外燈；色譜儀；色譜柱等更詳細信息請登錄上海武昊公司www.wuhaosh.com 或致電咨詢

    
 ELISA實驗前，血清、血漿、組織、細胞培養(yǎng)上清等樣品的采集及處理

 Serum - Use a serum separator tube and allow samples to clot for two hours at room temperature or overnight at 4oC before centrifugation for 20 minutes at approximay 1000×g. Assay freshly prepared serum immediay or store samples in aliquot at -20oC or -80oC for later use. Avoid repeated freeze/thaw cycles.

Plasma - Collect plasma using EDTA or heparin as an anticoagulant. Centrifuge samples for 15 minutes at  000×g at 2 - 8oC within 30 minutes of collection. Remove plasma and assay immediay or store samples in aliquot at -20oC or -80oC for later use. Avoid repeated freeze/thaw cycles.

Tissue homogenates  - The preparation of tissue homogenates will vary depending upon tissue type. For this assay, tissues were rinsed in ice-cold PBS（0.02mol/L,pH 7.0-7.2） to remove excess blood thoroughly and weighed before homogenization. Minced the tissues to small pieces and homogenized them in 5-10 mL of PBS with a glass homogenizer on ice（Micro Tissue Grinders woks, too）. The resulting suspension was sonicated with an ultrasonic cell disrupter or subjected to two freeze-thaw cycles to further break the cell membranes. After that, the homogenates were centrifugated for 5 minutes at 5000×g. Remove the supernate and assay immediay or aliquot and store at ≤-20oC.

Cell culture supernates    - Centrifuge samples for 20 minutes at 1000×g. Remove particulates and assay immediay or store samples in aliquot at -20oC or -80oC for later use. Avoid repeated freeze/thaw cycles.