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北京西美杰科技有限公司
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A1092,0025Coomassie® Brilliant blue R-250 （C.I. 42660）考馬斯亮藍(lán)
| 參考價(jià) | 面議 |
具體成交價(jià)以合同協(xié)議為準(zhǔn)
- 型號(hào) A1092,0025
- 品牌
- 廠商性質(zhì) 代理商
- 所在地 上海市
更新時(shí)間：2017-11-06 07:08:58瀏覽次數(shù)：1092
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| Synonym | Brilliant blue R, Xylenebrilliantcyanine | |||
|---|---|---|---|---|
| Formula | C45H44N3NaO7S2 | |||
| M | 825.98 g/mol | |||
| CAS-No. | 6104-59-2 | |||
| HS-No. | 32041200 | |||
| EC-No. | 228-060-5 | |||
| Storage | RT | |||
| LGK | 10 - 13 | |||
| WGK | 2 | |||
| ® registered trademark of Imperial Industries PLC | ||||
| Specification | ||||
| λmax. （buffer pH 7.0） | 554 - 563 nm | |||
| E 1 %,1 cm, λmax. | >300 （pH 7.0） | |||
（1） Fazekas De St. Groth, S. et al. （1963） Biochim. Biophys. Acta 71, 377-391
Two new staining procedures for quantitative estimation of proteins on electrophoresis strips.
（2） Chrambach, A. et al. （1967） Anal. Biochem. 20, 150-154
A procedure for rapid and sensitive staining of protein fractionated by polyacrylamide gel electrophoresis.
（3） Neuhoff, V. et al. （1988） Electrophoresis 9, 255-262
Improved staining of proteins in polyacrylamide gels including isoelectric focusing gels with clear background at nanogram sensitivity using Coomassie® Briliant Blue G-250 and R-250.
（4） Choi, J.-K. et al. （1996） Anal. Biochem. 236, 82-84
Modified Coomassie® Blue staining of proteins in polyacrylamide gels with Bismark brown.
（5） Tal, M. et al. （1985） J. Biol. Chem. 260, 9976-9980
Why does Coomassie® Brilliant Blue R Interact Differently with Different Porteins?
Two new staining procedures for quantitative estimation of proteins on electrophoresis strips.
（2） Chrambach, A. et al. （1967） Anal. Biochem. 20, 150-154
A procedure for rapid and sensitive staining of protein fractionated by polyacrylamide gel electrophoresis.
（3） Neuhoff, V. et al. （1988） Electrophoresis 9, 255-262
Improved staining of proteins in polyacrylamide gels including isoelectric focusing gels with clear background at nanogram sensitivity using Coomassie® Briliant Blue G-250 and R-250.
（4） Choi, J.-K. et al. （1996） Anal. Biochem. 236, 82-84
Modified Coomassie® Blue staining of proteins in polyacrylamide gels with Bismark brown.
（5） Tal, M. et al. （1985） J. Biol. Chem. 260, 9976-9980
Why does Coomassie® Brilliant Blue R Interact Differently with Different Porteins?
Coomassie® Brilliant Blue R-250 is one of the most commonly used stains for proteins, after their separation by polyacrylamide gel electrophoresis. The protein-dye complex has an absorption maximum at 549 nm, the dye without protein at 555 nm （in 0.01 M citrate buffer, pH 3）. The intensity in staining of proteins probably depends on the basicity of a protein （5）. Per positively charged amino acid approximay 1.5 - 3 molecules of Coomassie® will be bound. This variation complicate the exact protein determination with albumin as a standard, since this protein contains more basic amino acids than many other proteins （5）.
There do exist many protocols for sensitive staining procedures with Coomassie® （e. g. ref. 3, 4）. The sensitivity reaches a limit at 25 ng protein （4）. We recommend the following protocol:
I. Staining solution: 0.1 % Coomassie® Brilliant Blue R-250 （Prod.-No. A1092）
20 % methanol （or ethanol）
10 % acetic acid
The SDS gel （without 'stacking gel'） is stained for 1 hour at 60°C or for 2 hours at 50°C or over night at RT.
II. Destaining solution: 20 % methanol （or ethanol）
10 % acetic acid
Destain the gel for 3 - 4 hours at 50 - 60°C. Add some sponges.
Subsequently wash the gel for 15 minuts in water and dry under vacuum at 60°C for 2 - 3 hours.
There do exist many protocols for sensitive staining procedures with Coomassie® （e. g. ref. 3, 4）. The sensitivity reaches a limit at 25 ng protein （4）. We recommend the following protocol:
I. Staining solution: 0.1 % Coomassie® Brilliant Blue R-250 （Prod.-No. A1092）
20 % methanol （or ethanol）
10 % acetic acid
The SDS gel （without 'stacking gel'） is stained for 1 hour at 60°C or for 2 hours at 50°C or over night at RT.
II. Destaining solution: 20 % methanol （or ethanol）
10 % acetic acid
Destain the gel for 3 - 4 hours at 50 - 60°C. Add some sponges.
Subsequently wash the gel for 15 minuts in water and dry under vacuum at 60°C for 2 - 3 hours.
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