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CITRIC ACID, TRISODIUM SALT, DIHYDR
TMB PLUS,液體,英文名稱:TMB PLUS 1-COMPONENT LIQUID,等級:生物技術級,包裝規(guī)格:100ML,CAS:N/A
K830
TMB PLUS, LIQUID 1-COMPONENT SUBSTRATE
Grade:BIOTECHNOLOGY GRADE
CAS Number: N/ASynonym(s): None
Molecular Weight: N/AMolecular Formula: N/A
Storage Condition: COLD
CodeSizePriceQuantity
K830-100ML
TMB Plus Liquid 1-Component Substrate is a ready-to-use soluble substrate for kinetic and endpoint assays of horseradish peroxidase (HRP) in microwell procedures, such as ELISA. Reaction of the substrate with HRP generates a free-radical cation that exhibits blue color with an absorption maximum at 653 nm. Acidification of the reaction by addition of sulfuric acid yields a yellow-colored diimine terminal oxidation product, which absorbs light at 450 nm. TMB Plus Liquid 1-Component Substrate is optimized with TMB and hydrogen peroxide concentrations that allow a linear response to typical HRP concentrations utilized in immunologic assays.
Highly sensitive, soluble substrate for HRP
Ready-to-use solution
Measure color formation as a function of time (650 nm) or at a fixed interval (650 nm or 450 nm)
TMB Plus Liquid 1-Component Substrate: Contains 2.08 mmol L-1 TMB, hydrogen peroxide, citric acid buffer pH 3.3 and additional proprietary, non-toxic stabilizers
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