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Ellman's Reagent, 5,5'-Dithio-bis-（2-nitrobenzoic Acid）介紹

Thermo Scientific BCA蛋白定量產(chǎn)品可以用于研究蛋白：蛋白間相互作用，衡量親和層析后的組分含量，估算從細胞裂解液里提取的膜蛋白含量和高通量篩選融合蛋白。

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Ellman's Reagent, 5,5'-Dithio-bis-（2-nitrobenzoic Acid）品牌介紹

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Thermo Scientific Pierce DTNB （Ellman's Reagent） reacts with sulfhydryl groups to yield a colored product, providing a reliable method to measure reduced cysteines and other free sulfhydryls in solution.

Ellman's Reagent （5,5'-dithio-bis-[2-nitrobenzoic acid]） is used to estimate sulfhydryl groups in a sample by comparing to a standard curve of a sulfhydryl-containing compound such as cysteine. Alternatively, sulfhydryl groups can be assayed using the extinction coefficient of TNB （14,150M-1cm-1 at 412nm）. Ellman's Reagent has been used for applications other than sulfhydryl estimation, such as for determining alkylthiols by HPLC using a pre-column derivatization procedure and to study thiols in the active site of several enzymes.

Highlights:

• Determine availability of sulfhydryls for labeling or crosslinking applications
• Assay by measuring the absorbance at 412nm or using cysteine as a standard
• Temporarily block sulfhydryls to perform chemistries on other protein functionalities

 Ellman's Reagent, 5,5'-Dithio-bis-（2-nitrobenzoic Acid）僅用于科研

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