本產(chǎn)品僅用于科研,不用于臨床
人胚肺二倍體細(xì)胞;HEL-1
形態(tài)特性 淋巴母細(xì)胞樣;單個或成簇生長
生長特性 懸浮生長
特征特性 該細(xì)胞是從在WEHI-3細(xì)胞條件化培養(yǎng)基中長期培養(yǎng)的DBA/2小鼠正常骨髓細(xì)胞建立的;細(xì)胞的持續(xù)生長依賴于WEHI-3細(xì)胞的條件化培養(yǎng)基。該細(xì)胞表達(dá)Thy-1.2 (24%)。
培養(yǎng)條件 DMEM-H: Dulbecco's Modified Eagle's Medium (DME H-21 4.5g/Liter Glucose) 10%FBS;IL-3
傳代方法 1:3傳代;3~4天1次。
傳代情況 C5
凍存條件人胚肺二倍體細(xì)胞;HEL-1基礎(chǔ)培養(yǎng)基+5%DMSO+20%FBS
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