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- quickzyme是歐洲著名的EILISA 試劑盒提供商，為很多大的試劑公司OEM試劑盒，其產(chǎn)品高質(zhì)量和高靈敏性及穩(wěn)定性得到各大實(shí)驗(yàn)室及制藥公司認(rèn)可，其Granzyme B ELISA KIT 早已經(jīng)被世界各大實(shí)驗(yàn)室使用，高品質(zhì)的質(zhì)量得到眾多客戶的選擇
- bace1（β-site app cleaving enzyme），即β-分泌酶，是淀粉樣前體蛋白（amyloid precursor protein，app）產(chǎn)生aβ的關(guān)鍵酶。bace1屬于ⅰ型跨膜蛋白，由501個(gè)氨基酸組成，其中46～460位氨基酸為功能活性區(qū)域。bace1通過(guò)水解app670met-671asp之間的肽鍵，生成670個(gè)氨基酸的可溶性n末端和包含完整aβ區(qū)的app跨膜c端。后者經(jīng)γ分泌酶進(jìn)一步酶切形成aβ。zui近的研究表明，bace1基因敲除小鼠腦內(nèi)的aβ水平可降低90%，且其生理表型基本正常，顯示了bace1抑制劑在ad治療中的廣闊前景［1］。通過(guò)酵母或昆蟲表達(dá)系統(tǒng)表達(dá)活性bace1在zui近已見報(bào)道［2，3］。然而，由于上述系統(tǒng)表達(dá)的蛋白量低，且純化流程復(fù)雜，不適合bace1抑制劑的大規(guī)模篩選。而bace1的原核表達(dá)可克服以上缺陷，為篩選特異性抑制劑及研究bace1結(jié)構(gòu)與功能關(guān)系提供大量材料，為zui終從根本上治療ad帶來(lái)希望。
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The QuickZyme hydroxyproline assay is used for the quantitative determinantion of hydroxyproline. Hydroxyproline often results from （acid） hydrolysis of collagen. The QuickZyme hydroxyproline assay has a colorimetric read-out and a fast and simple assay format （< 2 hrs）.
Assay specifications
- Quantitative measurement of hydroxyproline.
- Samples: acid hydrolized tissue culture supernatants, cellular extracts, tissue homogenates, tissues.
- Colorimetric measurement
- Range: 6 to 300 μM. Sensitivity: 2.4 µM
- Uses hydroxyproline as standard
- Ease-of-use: Equivalent to ELISA
- Store at room temperature
- This kit does not contain tubes and a protocol for collagen hydrolysis. If you need these items please order the
| QZBhypro1 | QuickZyme Hydroxyproline Assay | 96T | 5980 | QuickZyme |
| QZBhypro2 | QuickZyme Hydroxyproline Assay | 96T*2 | 11270 | QuickZyme |
| QZBhypro5 | QuickZyme Hydroxyproline Assay | 96T*5 | 22770 | QuickZyme |
