9
IRDye® 800CW Carboxylate 羧基IR染料，西安瑞禧生物科技有限公司可以提供近紅外熒光染料
IRDye® 800CW Carboxylate 羧基IR染料，西安瑞禧生物科技有限公司可以提供近紅外熒光染料
IRDye® 800CW Carboxylate 羧基IR染料，西安瑞禧生物科技有限公司可以提供近紅外熒光染料
IRDye® 800CW Carboxylate 羧基IR染料
Assays that use IRDye® 800CW conjugates (such asin vivo imaging and cell binding assays) may require a "dye-only" control for potential effects or retention of the dye.
Carboxylate (non-reactive) form of IRDye 800CW is an ideal control.
Note: The carboxylate dye has no reactive group and cannot be used for labeling.
[Carboxylate clearance time course]
[ABOVE] Clearance kinetics of IRDye 800CW carboxylate. A single SCID mouse was injected with 1 nmol of non-reactive IRDye 800CW dye, and clearance monitored over time as indicated. Pseudocolor fluorescence is superimposed on a white light image. IRDye 800CW carboxylate dispersed rapidly, and was compley cleared after 48 h.
Originally published in Kovar et al, Anal Biochem 367:1-12 (2007).
How is IRDye 800CW carboxylate used?
In vitro cell-based assays
As a control for cell-based assays that monitor binding of a dye-labeled agent.
Validation of optical agents for in vivo administration
Evaluation of binding specificity
In vivo imaging
For evaluation of behavior and clearance of the dye itself
Timing of dye clearance from the animal's body
Retention of dye in certain organs or sites (e.g., liver or kidneys)
Labeling Reaction Reference
As a standard to determine the amount of unreacted ("free") dye after IRDye 800CW conjugation and purification.
Residual unreacted dye may cause:
Artificially high values when dye/protein (D/P) ratio is calculated
Increased background fluorescence in biological assays
