豬瘟病毒抗體檢測(cè)試劑盒

Classical Swine Fever Virus Antibody Test Kit (CSFV Ab)

概述

豬瘟病毒（CSFV）、牛病毒性腹瀉性病毒(BVDV)以及邊界?。˙DV）都屬于黃病毒屬的成員。由于CSFV的高致病力和高致死率，它已給養(yǎng)殖業(yè)造成了巨大的損失。豬感染高致病力毒株后在潛伏期就可以造成傳染，經(jīng)歷急性豬瘟或亞急性豬瘟臨床癥狀的豬在出現(xiàn)癥狀前大量排毒，但耐過后的豬只體內(nèi)有明顯的CSFV抗體，且不再排毒。感染溫和性毒株的豬呈慢性感染，在病死前會(huì)長(zhǎng)期或間歇性地排毒。懷孕母豬可以通過胎盤感染胎兒導(dǎo)致流產(chǎn)、木乃伊化或產(chǎn)出弱豬、畸形胎等。先天性感染低毒力病毒的結(jié)果是產(chǎn)出持續(xù)性感染的仔豬，仔豬出現(xiàn)免疫耐受，不表現(xiàn)任何癥狀向外排毒。豬也有可能感染BVDV或BDV。盡管這些感染通常呈溫和過程并且是自限性的，但有效的將它們同豬瘟病毒區(qū)分是很重要的。

原理

豬瘟病毒抗體ELISA檢測(cè)試劑盒是用來(lái)檢測(cè)豬血清或血漿中豬瘟病毒抗體的檢測(cè)試劑盒。該試劑盒是用豬瘟病毒抗原包被的微量反應(yīng)板，利用阻斷ELISA原理來(lái)檢測(cè)豬血清或血漿中豬瘟病毒抗體。如果被檢樣品中存在豬瘟病毒抗體，它們就會(huì)阻斷辣根過氧化物酶標(biāo)記（HRPO）的抗豬瘟病毒的單克隆抗體。單克隆抗體與豬瘟病毒抗原的結(jié)合可以通過辣根過氧化物酶與底物的顯色程度進(jìn)行判定，即用酶標(biāo)儀在單波長(zhǎng)450nm或雙波長(zhǎng)450nm與620nm測(cè)定該反應(yīng)體系的吸光度。當(dāng)被檢樣品中含有豬瘟病毒抗體（陽(yáng)性結(jié)果）時(shí)，顯色就會(huì)變淺，當(dāng)被檢樣品中不含有抗豬瘟病毒抗體（陰性結(jié)果）時(shí)，顯色就會(huì)變深。樣本的阻斷率可以通過450nm波長(zhǎng)樣本吸光度與陰性對(duì)照吸光度的比值來(lái)確定。

The PrioCHECK? CSFV Antibody 2.0 ELISA is a blocking ELISA that specifically detects antibodies against the A domain of the E2 protein of CSFV. This domain is more specific for CSFV than the B, C, and D domain of the E2 protein. An important advantage of the test is that it does not cross-react with related viruses such as Bovine Viral Diarrhoea virus (BVDV) and Border Disease virus (BDV). The test detects antibodies against high-, moderate-, and low-virulent CSFV strains early after infection. Detection of antibodies elicited by low-virulent CSFV strains is of paramount importance to trace sub clinical CSFV infections.

Test procedure
The PrioCHECK? CSFV Antibody 2.0 ELISA is a blocking ELISA. In this test, the plates are coated with the E2 CSFV envelope protein. The key reagent is a monoclonal antibody (mAb) directed against an epitope located on the A domain of the envelope protein E2 of CSFV, making the test more specific for CSFV. The mAb is labeled with an enzyme that generates a color signal. The signal is measured and when no color is formed, the sample containing anti-CSFV antibodies that competed for the A domain of the E2 protein is positive for CSFV.