瑞士Prionics公司 華南地區(qū)總代理 廣州測迪生物科技有限公司

Prionics Parachek牛羊副結(jié)核ParaTB 抗體檢測ELISA 試劑盒

Parachek試劑盒，應(yīng)用間接ELISA方法，檢測牛，綿羊，山羊的血清或者奶樣中副結(jié)核分枝桿菌抗體。Parachek僅僅需要4個步驟大概80分鐘就能完成整個實驗。
Parachek是*獲得USDA批準(zhǔn)應(yīng)用于奶樣品檢測副結(jié)核分枝桿菌抗體的試劑盒，診斷副結(jié)核病的有力工具，也能幫助廣大農(nóng)場工作人員用于DHI中對副結(jié)核的評估。

PARACHEK? 2 is a solid phase, indirect enzyme immunoassay (EIA) to detect antibodies to M. paratuberculosis in animal sera and milk of cattle, sheep, and goats. The PARACHEK? 2 procedure for 96 determinations (including controls) takes approximay 80 min and consists of four steps. In the first step, the samples are diluted and incubated in a diluent buffer containing M. phlei to remove cross-reacting antibodies. In the second step, specific antibodies present in the diluted samples bind to M. paratuberculosis antigens coated to the wells of a microtiterplate. Unreacted proteins are removed by washing. A secondary antibody that is conjugated to an enzyme that generates a color signal is added in the third step. In the fourth step, the presence of specific antibodies is visualized by adding the enzyme substrate. The intensity of the color signal is proportional to the amount of antibodies in the sample.

Parachek? is the first USDA approved Johne’s disease test for bovine milk.
Prionics, a world leader in farm animal diagnostics, and An BioSystems, Inc. have received United States Department of Agriculture (USDA) approval for the extension of use of the Parachek? test for diagnosis of Johne’s disease in bovine milk samples. It is the first ELISA kit approved by the USDA for testing bovine milk. Parachek?, the original Johne’s ELISA kit, is widely used for the detection of antibodies to Mycobacterium paratuberculosis in serum of cattle, sheep and goats.

As a part of their collaboration, An BioSystems, Inc. (AnBio) and Prionics submitted data to USDA’s Center for Veterinary Biologics last fall to obtain the dual claim for Prionics’ Parachek? kit. With the added USDA claim, Prionics and AnBio sought to have milk testing officially acknowledged in the U.S. Voluntary Johne’s Control Program. The integration of milk testing in state and nationally-funded Johne’s programs will have an immediate and positive impact on the level of participation of milk producers. These programs have been designed to address the growing concern about Johne’s disease.

The Parachek? milk test will enable dairy producers to use milk samples -- routinely collected as part of the Dairy Herd Improvement (DHI) system -- for Johne’s diagnosis. Since these milk samples are already collected on a regular basis for nearly half the dairy producers in the U.S. and Canada, the cost and inconvenience of sample collection and shipping associated with serum testing is eliminated. The potential to integrate herd health data into DHI could also significantly enhance the ability to track and manage Johne’s disease. 

由于患病動物可能沒有臨床癥狀，因此感染往往無法被發(fā)現(xiàn)。但通過進(jìn)行靈敏而經(jīng)濟的ELISA檢測和執(zhí)行副結(jié)核控制計劃，牛場管理者可準(zhǔn)確鑒別副結(jié)核感染，從而提高畜群健康水平并增加收益。