廣州測迪生物科技有限公司
主營產(chǎn)品: 美國kernel試劑盒,德國拜發(fā)試劑盒,瑞士Prionics試劑盒,荷蘭賽迪試劑盒 |
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廣州測迪生物科技有限公司
主營產(chǎn)品: 美國kernel試劑盒,德國拜發(fā)試劑盒,瑞士Prionics試劑盒,荷蘭賽迪試劑盒 |
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荷蘭賽迪/測迪 ceditest 瑞士Prionics公司 華南地區(qū)總代理 廣州測迪生物科技有限公司
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豬旋毛蟲抗體檢測試劑盒
7610150 PrioCHECK? Trichinella Antibody ELISA Kit,
The PrioCHECK? Trichinella Antibody ELISA is a highly sensitive and specific ELISA for the detection of antibodies against Trichinella in serum and meat juice samples of pigs. No cross reactivity with commonly found pig parasites has been observed.
Test procedure
The PrioCHECK? Trichinella Antibody ELISA is a diagnostic kit based on ELISA technology. It follows a four-step protocol consisting of sample preparation, sample incubation, conjugate incubation, and detection. Serum or meat juice samples are incubated in an ELISA plate coated with the Trichinella E/S antigen. Anti-Trichinella antibodies present in the sample bind to the antigen. Plates are then washed and an anti-swine antibody labeled with horse radish peroxidase (HRP) is used to detect antibodies bound to the E/S antigen. Color development using mits tetramethyl-benzidine (TMB) substrate measured optically at a wavelength of 450 nm shows the presence of antibodies directed against Trichinella spp.
旋毛蟲癥是一種通常由線蟲類旋毛蟲引起的人獸共患傳染病。通常,它對于動物來說是良性的,而對于人類則是一種嚴重的疾病,當(dāng)蛔蟲從腸內(nèi)轉(zhuǎn)移至肌肉組織時可導(dǎo)致腸部的癥狀和肌肉疼痛。
人類通過攝取未煮熟的感染肉(以豬肉zui為常見)而受到感染。因此,鑒定感染動物對于公共衛(wèi)生而言至關(guān)重要。
該病診斷是基于在屠宰時直接檢測幼蟲,通過旋毛蟲鏡檢法或肌肉檢測法或血清學(xué)篩查以檢測旋毛蟲抗體spp而實現(xiàn)的。旋毛蟲血清ELISA可檢測出豬、野豬的血清和肉汁中的旋毛蟲、偽旋毛蟲、布氏旋毛蟲和北方旋毛蟲的抗體。