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新品速遞---“一步法"建庫試劑盒
-----Hieff NGS® OnePot DNA Library Prep Kit for Illumina®
? 產(chǎn)品背景:
DNA文庫的構(gòu)建是二代測序前期的核心環(huán)節(jié)。目前市面上推廣的建庫試劑盒主要有常規(guī)建庫試劑盒以及轉(zhuǎn)座酶法快速建庫試劑盒。常規(guī)試劑盒需要購買單獨的片段化模塊或者采用機械法進行片段化,機械法片段化對DNA分子有一定的損傷,影響文庫質(zhì)量。轉(zhuǎn)座酶法試劑盒都是針對特定Input DNA量的樣本進行建庫,并且存在一定的偏好性,影響測序質(zhì)量。
Hieff NGS® OnePot DNA Library Prep Kit是針對Illumina高通量測序平臺專業(yè)開發(fā)設(shè)計的新一代酶切法建庫試劑盒。與傳統(tǒng)的建庫法比較,本品采用高質(zhì)量的酶學組成,擺脫了繁瑣的超聲過程,解決您的難題。
1.適用500 pg-100 ng的基因組DNA、cDNA等樣本;
2.一管式操作,片段化,末端修復(fù)/A尾添加,接頭連接三步實現(xiàn)一管操3.作,簡單;
4.高質(zhì)量片段化酶,優(yōu)化的緩沖體系,具有穩(wěn)定的片段化效果;
5.強擴增效率的高保真酶,顯著提高文庫轉(zhuǎn)化率與擴增效率;
6.多樣本驗證可獲得優(yōu)異的文庫與測序數(shù)據(jù)。
? 適用范圍:
常規(guī)動植物基因、微生物基因組DNA等樣本的建庫。
? 性能展示:
高質(zhì)量的片段化酶引入,極大降低偏好性
強擴增效率的高保真酶,保證文庫測序均一性
不同深度下測序數(shù)據(jù),*
Species | GC % | Mapped Reads % | Coverage % | Coverage Depth % | Quality % | ||||
10× | 50× | 100× | 150× | Q20 | Q30 | ||||
蠟狀芽孢桿菌 | 34 | 93.73 | 99.64 | 99.64 | 99.62 | 99.58 | 98.8 | 96.29 | 91.73 |
大腸桿菌 | 50 | 98.98 | 100 | 100 | 99.98 | 99.95 | 97.12 | 96.97 | 92.39 |
嗜鹽桿菌 | 65 | 94.15 | 100 | 100 | 99.98 | 99.91 | 99.89 | 96.46 | 91.72 |
? 產(chǎn)品訂購:
產(chǎn)品 | 貨號 | 規(guī)格 | 價格(元) |
Hieff NGS® OnePot DNA Library Prep Kit for Illumina® | 12203ES08 | 8 T | 3656.00 |
12203ES24 | 24 T | 7486.00 | |
12203ES96 | 96 T | 28567.00 |
? 相關(guān)產(chǎn)品:
>>>建庫試劑盒 | ||||
類別 | 產(chǎn)品名稱 | 貨號 | 規(guī)格 | 價格 (元) |
快速流程 | Hieff NGS® MaxUp II DNA Library Prep Kit for Illumina® | 12200ES24/96 | 24/96 T | 6486/23567 |
一步法建庫流程 | Hieff NGS® OnePot DNA Library Prep Kit for Illumina® | 12203ES24/96 | 24/96 T | 7486/28567 |
鏈特異性mRNA | Hieff NGS® MaxUp Dual Mode mRNA Library Prep Kit for Illumina® | 12301ES24/96 | 24/96 T | 9756/32856 |
>>>磁珠類 | ||||
分選100-200 bp DNA | Hieff NGS® cfDNA Clean Beads(100~200bp) | 12599ES08/56 | 5/60 mL | 1666/9106 |
回收50 bp以上DNA | Hieff NGS® Smarter DNA Clean beads (50bp以上) | 12600ES56/75 | 60/450 mL | 7586/29886 |
等同AMPure XP | Hieff NGS® DNA Selection Beads(Superior AMPure XP alternative) | 12601ES56/75 | 60/450 mL | 6286/26186 |
總RNA純化 | Hieff NGS® RNA Cleaner | 12602ES56/75 | 60/450 mL | 4266/38566 |
mRNA分離 | Hieff NGS® mRNA Isolation Master Kit | 12603ES24/96 | 24/96 T | 616/2266 |
>>>建庫接頭 | ||||
完整接頭48種Index | Hieff NGS® Complete Adapter Kit for Illumina®, Set 1/2 | 12615/12616ES04/16 | 12×4/12×16 T | 1256/4536 |
Hieff NGS® Complete Adapter Kit for Illumina®, Set 3/4 | 12617/12618ES04/16 | 12×4/12×16 T | 1256/4536 | |
短接頭 | Hieff NGS® 96 Single Index Primers Kit for Illumina®, Set 1/2 | 12611/12612ES02 | 48×2 T | 1493 |
Hieff NGS® 384 Dual Index Primers Kit for Illumina®, Set 1/2 | 12613/12614ES02 | 96×2 T | 2753 | |
96種Index | Hieff NGS® Tagment Index Kit for Illumina® (96 Index) | 12610ES96 | 96 T | 1365 |
>>>文庫定量 | ||||
qPCR Mix | Hieff NGS® Library Quantification Kit for Illumina®, qPCR Master Mix | 12302ES05 | 500 T | 1526 |
qPCR標準品 | Hieff NGS® Library Quantification Kit for Illumina®, DNA Standard | 12307ES09 | 6×96 μL | 2126 |
Qubit法 | dsDNA HS Assay Kit for Qubit® | 12640ES05/09 | 100/500 T | 686/2696 |
>>>建庫模塊 | ||||
片段化 | Hieff NGS® Fast-Pace DNA Fragmentation Reagent | 12609ES50 | 50 T | 1466 |
末端修復(fù)/加A | Hieff NGS® Fast-Pace End Repair/dA-Tailing Module | 12608ES24/96 | 24/96 T | 2066/6166 |
快速連接 | Hieff NGS® Fast-Pace DNA Ligation Module | 12607ES24/96 | 24/96 T | 3263/10863 |
文庫擴增 | 2×Super Canace®Ⅱ High-Fidelity Mix for Library Amplification | 12621ES24/96 | 24/96 T | 583/1783 |
逆轉(zhuǎn)錄 | Hieff NGS® Dual-mode cDNA Synthesis Kit | 12250ES24/96 | 24/96 T | 4885/16485 |
>>>多重PCR定制咨詢 | ||||
>>>文庫構(gòu)建原料酶咨詢 |