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新品推薦|反轉(zhuǎn)、高質(zhì)量cDNA,用Yeasen第三代逆轉(zhuǎn)錄酶預(yù)混液
你的逆轉(zhuǎn)錄,交給YEASEN三代逆轉(zhuǎn)錄酶來守護 !
Hifair® Ⅲ 1st Strand cDNA Synthesis SuperMix for qPCR (gDNA digester plus)是基于翊圣Hifair® Ⅲ Reverse Transcriptase而開發(fā)的即用型預(yù)混液。該產(chǎn)品可耐受高達65℃的反應(yīng)溫度,適合具有復(fù)雜二級結(jié)構(gòu)的RNA模板的逆轉(zhuǎn)錄;同時增強了與模板的親和力,非常適合少量模板以及低拷貝基因的逆轉(zhuǎn)錄。
產(chǎn)品帶基因組DNA去除成分,適用于兩步法RT-qPCR檢測,針對qPCR進行特別優(yōu)化,比例優(yōu)化的Random primers/ Oligo (dT)18 primer mix,使cDNA合成可從RNA轉(zhuǎn)錄本的各個區(qū)域起始,并具有相同的逆轉(zhuǎn)錄效率,zui大程度保證了qPCR結(jié)果的真實性和可重復(fù)性。
產(chǎn)品特點
1.耐熱性
耐受65℃,適合高GC及具有復(fù)雜二級結(jié)構(gòu)的RNA模板
2.兼容性
適合不同GC含量、不同表達豐度基因逆轉(zhuǎn)錄
3.線性檢測范圍
在寬廣模板范圍內(nèi)(Total RNA 10pg-5μg)均可合成cDNA
4.應(yīng)用范圍
一管多用,適用于后續(xù)qPCR及常規(guī)長度基因克隆實驗
5.模板投入量
RNA投入量多可加12μL,有利于低濃度模板檢測
6.操作簡便性
基因組DNA去除與逆轉(zhuǎn)錄均設(shè)置成mix形式,加樣方便,移液誤差小
7.反應(yīng)時間
逆轉(zhuǎn)錄時間15min,反應(yīng)總時間低于半小時
產(chǎn)品應(yīng)用
兩步法RT-qPCR檢測。
產(chǎn)品性能
1.反應(yīng)溫度55℃,耐受65℃高溫
逆轉(zhuǎn)錄溫度55℃,高GC模板可適當(dāng)提高到65℃反應(yīng)
2.適合不同GC含量、不同表達豐度基因逆轉(zhuǎn)錄
適合低豐度基因逆轉(zhuǎn)錄,適合不同GC含量(25-65%)基因逆轉(zhuǎn)錄
3.線性檢測范圍廣,Total RNA 10pg-5μg
11141在很寬的模板范圍內(nèi)(Total RNA 10pg-5μg)均可合成cDNA
4.一管多用,適用于后續(xù)qPCR及常規(guī)長度基因克隆實驗
5.RNA大投入12μL,有利于低濃度模板檢測
YEASEN逆轉(zhuǎn)錄產(chǎn)品部分已發(fā)表文獻
1. Wang J, Ge P, Qiang L, et al. The mycobacterial phosphatase PtpA regulates the expression of host genes and promotes cell proliferation[J]. Nature communications, 2017, 8(1): 244. [ IF 12.353]
2. Zhu Y, Song D, Xu P, et al. A HD‐ZIP III gene, PtrHB4, is required for interfascicular cambium development in Populus[J]. Plant biotechnology journal, 2018, 16(3): 808-817. [ IF 6.305]
3. Yu S L, Wu J C, Liu P F, et al. Up-regulation of RNF187 induces hepatocellular carcinoma cell epithelial to mesenchymal transitions[J]. Oncotarget, 2017, 8(60): 101876. [ IF 6.18]
4. Xu Y, Zhang G, Zou C, et al. LncRNA MT1JP suppresses gastric cancer cell proliferation and migration through MT1JP/MiR-214-3p/RUNX3 axis[J]. Cellular Physiology and Biochemistry, 2018, 46(6): 2445-2459.[ IF 5.5]
5. Li J, Yang Y L, Li L Z, et al. Succinate accumulation impairs cardiac pyruvate dehydrogenase activity through GRP91-dependent and independent signaling pathways: Therapeutic effects of ginsenoside Rb1[J]. Biochimica et Biophysica Acta (BBA)-Molecular Basis of Disease, 2017, 1863(11): 2835-2847.[ IF 5.108]
產(chǎn)品訂購
產(chǎn)品名稱 | 產(chǎn)品編號 | 規(guī)格 | 原價(元) | *(元) |
Hifair® Ⅲ 1st Strand cDNA Synthesis Super Mix for qPCR (gDNA digester plus) | 11141ES60 | 100 T | 1383 | 798 |
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