聯(lián)系電話
- 聯(lián)系人:
- 曹女士
- 電話:
- 400-6111-883
- 手機(jī):
- 售后:
- 4006-111-883
- 傳真:
- 86-21-34615995
- 地址:
- 上海市浦東新區(qū)天雄路166弄1號3樓
- 網(wǎng)址:
- www.yeasen.com
掃一掃訪問手機(jī)商鋪
1月,登《Journal of Experimental & Clinical Cancer Research》(IF:11.161);
2月,再登《Clinical and Translational Medicine》(IF:11.492);
3月,又登《Journal of Experimental & Clinical Cancer Research》(IF:11.161);
連續(xù)3個月都發(fā)表影響因子10+的高水平文章,充分展現(xiàn)了南京中醫(yī)藥大學(xué)楊燁和顧春艷教授團(tuán)隊(duì)強(qiáng)大的科研實(shí)力。
值得一提的是,在這些研究中,該團(tuán)隊(duì)都選擇了翌圣的轉(zhuǎn)染試劑產(chǎn)品用于相關(guān)基因的細(xì)胞轉(zhuǎn)染:
目前轉(zhuǎn)染試劑系列的產(chǎn)品已經(jīng)榮登《Nature》 《Cell》等多個頂級期刊,獲得科研大牛們認(rèn)可!
以下僅展示部分論文:
[1] Liu R, Yang J, et al. Optogenetic control of RNA function and metabolism using engineered light-switchable RNA-binding proteins. Nat Biotechnol. 2022 Jan 3. (IF:55)華東理工大學(xué)生物工程學(xué)院楊弋團(tuán)隊(duì)
[2] Zhou J, Chen P, et al. Cas12a variants designed for lower genome-wide off-target effect through stringent PAM recognition. Mol Ther. 2022 Jan 5.(IF:11.454)武漢大學(xué)生命科學(xué)學(xué)院殷雷團(tuán)隊(duì)
[3] Chen S, Cao X, et al. circVAMP3 Drives CAPRIN1 Phase Separation and Inhibits Hepatocellular Carcinoma by Suppressing c-Myc Translation. Adv Sci (Weinh). 2022 Jan 24.(IF:16.808)中國科學(xué)院北京生命科學(xué)研究院趙方慶團(tuán)隊(duì)
[4] Gu C, Wang Y, et al. AHSA1 is a promising therapeutic target for cellular proliferation and proteasome inhibitor resistance in multiple myeloma. J Exp Clin Cancer Res. 2022 Jan 6.(11.161)南京中醫(yī)藥大學(xué)楊燁顧春艷團(tuán)隊(duì)[5] Zhang Y, Yu X, et al. Splicing factor arginine/serine-rich 8 promotes multiple myeloma malignancy and bone lesion through alternative splicing of CACYBP and exosome-based cellular communication. Clin Transl Med. 2022 Feb.(11.492)南京中醫(yī)藥大學(xué)楊燁顧春艷團(tuán)隊(duì)
[6] Qin J, Cai Y, et al. Molecular mechanism of agonism and inverse agonism in ghrelin receptor. Nat Commun. 2022 Jan 13.(14.9)四川大學(xué)生物治療國家重點(diǎn)實(shí)驗(yàn)室邵振華團(tuán)隊(duì)
[7] Tang X, Deng Z, et al. A novel protein encoded by circHNRNPU promotes multiple myeloma progression by regulating the bone marrow microenvironment and alternative splicing. J Exp Clin Cancer Res. 2022 Mar 8.(11.161)南京中醫(yī)藥大學(xué)楊燁顧春艷團(tuán)隊(duì)
[8] Xie F, Su P, et al. Engineering Extracellular Vesicles Enriched with Palmitoylated ACE2 as Therapy. Adv Mater. 2021 Oct 19. (IF:30.849)蘇州大學(xué)生物醫(yī)學(xué)研究院周芳芳團(tuán)隊(duì)和浙江大學(xué)生命科學(xué)研究院張龍團(tuán)隊(duì)
[10] Fan Y, Wang J, et al. CircNR3C2 promotes HRD1-mediated tumor-suppressive effect via sponging miR-513a-3p in triple-negative breast cancer. Mol Cancer. 2021 Feb 2.(IF:27.403)南京醫(yī)科大學(xué)附屬逸夫醫(yī)院蘇東明團(tuán)隊(duì)
產(chǎn)品描述
產(chǎn)品特點(diǎn)
高效性:適合瞬時轉(zhuǎn)染或者穩(wěn)定轉(zhuǎn)染細(xì)胞系。
低毒性:轉(zhuǎn)染細(xì)胞保持很好的活性。
適應(yīng)性廣:普通細(xì)胞、難轉(zhuǎn)染的原代細(xì)胞、活體全面覆蓋。
操作簡便:適合血清存在的培養(yǎng)基,轉(zhuǎn)染前后無需更換培養(yǎng)基。
性價比高:經(jīng)濟(jì)實(shí)用,轉(zhuǎn)染效率高,價格低
數(shù)據(jù)展示
Hieff Trans™脂質(zhì)體核酸轉(zhuǎn)染試劑是一種多用途的脂質(zhì)體轉(zhuǎn)染試劑,適用于DNA、RNA和寡核苷酸的轉(zhuǎn)染,對大多數(shù)真核細(xì)胞具有很高的轉(zhuǎn)染效率。其*的配方使其可直接加入培養(yǎng)基中,血清的存在不會影響轉(zhuǎn)染效率,這樣可以減少去除血清對細(xì)胞的損傷。轉(zhuǎn)染后不需要除去核酸-Hieff Trans™復(fù)合物或更換新鮮培養(yǎng)基,也可在4~6小時后除去。
PEI 40000是一種分子量為40000的高電荷陽離子聚合物,非常容易結(jié)合帶負(fù)電荷的核酸分子,形成復(fù)合物,并使該復(fù)合物進(jìn)入細(xì)胞中。PEI 40000是一種瞬時轉(zhuǎn)染試劑,細(xì)胞毒性低,轉(zhuǎn)染效率高,在HEK293和CHO等細(xì)胞中基因表達(dá)效率較高。目前已經(jīng)驗(yàn)證線性PEI轉(zhuǎn)染試劑廣泛適用于多種細(xì)胞系包括HEK-293、HEK293T、CHO-K1、COS-1、COS-7、NIH/3T3 、Sf9、HepG2和Hela細(xì)胞等。轉(zhuǎn)染效率高達(dá)80%~90%。