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REPOTMNF-κB-GFP報告基因主要應(yīng)用于NF-κB信號通路的研究和基于NF-κB信號通路的藥物研究以及癌細(xì)胞中NF-KB的調(diào)控機制的研究
REPOTMNF-KB-GFP報告基因
REPOTMNF-kB-GFP報告基因是吉滿生物自主研發(fā)的用于檢測NFκB轉(zhuǎn)錄活性水平為目的的報告基因。NF-κB基因在炎癥反應(yīng)、免疫應(yīng)答和抑制細(xì)胞凋亡等方面有著重要的作用。
REPOTMNF-κB-GFP報告基因主要應(yīng)用于NF-κB信號通路的研究和基于NF-κB信號通路的藥物研究以及癌細(xì)胞中NF-KB的調(diào)控機制的研究。
pGMNF-ΚB-GFP是吉滿生物改造后的哺乳動物真核表達載體,在其多克隆位點插入了多個NF-κB結(jié)合位點,可以高效地檢測NF-κB的激活水平。由于載體采用了GFP作為報告基因,更便于后續(xù)的檢測。同時,對載體中預(yù)測出的其它轉(zhuǎn)錄因子以外的結(jié)合位點進行了適當(dāng)?shù)耐蛔?,增加了質(zhì)粒的轉(zhuǎn)錄因子結(jié)合特異性。另外,由于質(zhì)粒體積減小,使得NF-κB-GFP報告基因更易于轉(zhuǎn)染。
質(zhì)粒圖譜:
包裝清單:
產(chǎn)品編號 | 產(chǎn)品名稱 | 包裝 |
GM-024001 | REPOTMNF-κB-GFP報告基因 | 1μg |
- | 說明書 | - |
使用說明:
pGMNF-κB -GFP可以采用常規(guī)轉(zhuǎn)染方法轉(zhuǎn)染哺乳動物細(xì)胞。
TNF-α、IL-1β和LPS等是常見的NF-κB激活劑,可作為NF-κB報告基因的陽性對照。
注意事項:
本質(zhì)粒未經(jīng)吉滿生物允許不得用于任何商業(yè)用途,也不得移交給訂貨人實驗室以外的任何人或單位。
為了您的健康,實驗操作時請穿實驗服和帶一次性手套。
保存條件:
-20℃保存。
參考文獻:
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Lipopolysaccharide and tumor necrosis factor regulate Parkin expression via nuclear factor-kappa B.
PLoS One. 2011;6(8):e23660. doi: 10.1371/journal.pone.0023660. Epub 2011 Aug 17.
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Chem Biol Interact. 2006 Feb 1;159(2):117-28. Epub 2005 Nov 10
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