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供貨周期 | 一周 | 貨號(hào) | 11745ES03 |
---|---|---|---|
應(yīng)用領(lǐng)域 | 生物產(chǎn)業(yè) |
SRE-GFP報(bào)告基因質(zhì)粒(Reporter Plasmid)
產(chǎn)品描述
SRE-GFP報(bào)告基因質(zhì)粒(Reporter Plasmid)是翌圣生物自主研發(fā)的用于檢測SRF轉(zhuǎn)錄活性水平為目的的報(bào)告基因。三重復(fù)合體TCR/Elk1/SRF結(jié)合到SRE進(jìn)行基因表達(dá)的調(diào)控。
SRE-GFP報(bào)告基因主要用于檢測細(xì)胞中MAPK/ERK信號(hào)通路、藥物研究、基因過表達(dá)和RNAi的表型分析等。
pGMSRE-GFP是翌圣生物改造后的哺乳動(dòng)物真核表達(dá)載體,在其多克隆位點(diǎn)插入了多個(gè)SRE結(jié)合位點(diǎn),可以高效地檢測SRE的激活水平。由于載體采用了GFP作為報(bào)告基因,更便于后續(xù)的檢測。同時(shí),對載體中預(yù)測出的其它轉(zhuǎn)錄因子以外的結(jié)合位點(diǎn)進(jìn)行了適當(dāng)?shù)耐蛔?,增加?/span>質(zhì)粒的轉(zhuǎn)錄因子結(jié)合特異性。另外,由于質(zhì)粒體積減小,使得SRE-GFP報(bào)告基因更易于轉(zhuǎn)染。
質(zhì)粒圖譜
質(zhì)粒元件信息
SRE response element (SRE) | 32-67 |
Minimal TA promoter (pTA) | 96-118 |
GFP reporter gene | 160-879 |
SV40 late poly(A) signal | 914-1135 |
SV40 early promoter | 1183-1600 |
Synthetic neomycin phosphotransferase(Neor) coding region | 1626-2419 |
Synthetic poly(A) signal | 2445-2492 |
Synthetic Beta-lactamase(Ampr) coding region | 3608-4467 |
Synthetic poly(A) signal/transcriptional pause site | 4573-4726 |
運(yùn)輸與保存方法
冰袋運(yùn)輸。-20℃保存。有效期1年。
使用說明
pGMSRE-GFP可以采用常規(guī)轉(zhuǎn)染方法轉(zhuǎn)染哺乳動(dòng)物細(xì)胞。
注意事項(xiàng)
1)本質(zhì)粒未經(jīng)翌圣生物允許不得用于任何商業(yè)用途,也不得移交給訂貨人實(shí)驗(yàn)室以外的任何人或單位。
2)為了您的健康,實(shí)驗(yàn)操作時(shí)請穿實(shí)驗(yàn)服和戴一次性手套。
3) 本產(chǎn)品僅作科研用途!
參考文獻(xiàn)
[1] Katsch K, et al. Actin-dependent activation of serum response factor in T cells by the viral oncoprotein tip. Cell Commun Signal. 10(1):5(2012).
[2] Gozé C, et al. Involvement of tryptophan W276 and of two surrounding amino acid residues in the high constitutive activity of the ghrelin receptor GHS-R1a. Eur J Pharmacol. 643(2-3):153-61(2010).
[3] Notcovich C, et al. Histamine acting on H1 receptor promotes inhibition of proliferation via PLC, RAC, and JNK-dependent pathways. Exp Cell Res. 316(3):401-11 (2010).
[4] Hawkins JL, et al. Pharmacologic inhibition of site 1 protease activity inhibits sterol regulatory element-binding protein processing and reduces lipogenic enzyme gene expression and lipid synthesis in cultured cells and experimental animals. J Pharmacol Exp Ther. 326(3):801-8(2008).
[5] Aminova LR, et al. The C3 domain of Pasteurella multocida toxin is the minimal domain responsible for activation of Gq-dependent calcium and mitogenic signaling. Protein Sci. 17(5):945-9(2008).
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HB210825