Rb Luciferase Reporter Plasmid

（Rb-Luc熒光素酶報(bào)告基因質(zhì)粒）

產(chǎn)品信息

產(chǎn)品描述

Rb-Luc熒光素酶報(bào)告基因（報(bào)告基因質(zhì)粒）（Rb luciferase reporter plasmid）是翊圣生物自主研發(fā)的用于檢測(cè)Retinoblastoma protein轉(zhuǎn)錄活性水平為目的的報(bào)告基因。Retinoblastoma protein(Rb)是一種腫瘤抑制蛋白，在多種癌細(xì)胞中是發(fā)生功能障礙的。Rb是通過(guò)抑制處于分裂期細(xì)胞的細(xì)胞周期進(jìn)程阻礙細(xì)胞的過(guò)度增殖。

Rb報(bào)告基因主要用于檢測(cè)細(xì)胞中cell cycle信號(hào)通路活性、藥物研究以及基因過(guò)表達(dá)和RNAi的表型分析等。

pGMRb-Lu是翊圣生物改造后的哺乳動(dòng)物真核表達(dá)載體，在其多克隆位點(diǎn)插入了多個(gè)Rb結(jié)合位點(diǎn)，可以高靈敏度地檢測(cè)Rb的激活水平。同時(shí)，對(duì)載體中預(yù)測(cè)出的其它轉(zhuǎn)錄因子以外的結(jié)合位點(diǎn)進(jìn)行了適當(dāng)?shù)耐蛔儯黾恿速|(zhì)粒的轉(zhuǎn)錄因子結(jié)合特異性。由于質(zhì)粒體積減小，使得Rb報(bào)告基因更易于轉(zhuǎn)染。

質(zhì)粒圖譜

運(yùn)輸與保存方法

冰袋（wet ice）運(yùn)輸。收到產(chǎn)品后放到-20oC保存。

注意事項(xiàng)

本質(zhì)粒未經(jīng)翊圣生物允許不得用于任何商業(yè)用途，也不得移交給訂貨人實(shí)驗(yàn)室以外的任何人或單位。

為了您的健康，實(shí)驗(yàn)操作時(shí)請(qǐng)穿實(shí)驗(yàn)服和帶一次性手套。

使用說(shuō)明

pGMRb-Lu可以采用常規(guī)轉(zhuǎn)染方法轉(zhuǎn)染哺乳動(dòng)物細(xì)胞。用熒光素酶檢測(cè)試劑盒或雙熒光素酶檢測(cè)試劑盒進(jìn)行檢測(cè)。

參考文獻(xiàn)

[1] Tsang WP ,et al. Oncofetal H19-derived miR-675 regulates tumor suppressor RB in human colorectal cancer. Carcinogenesis. 2010 Mar;31(3):350-8.

[2] Niesen MI ,et al. Technological advances in the study of HLA-DRA promoter regulation: extending the functions of CIITA, Oct-1, Rb, and RFX. ActaBiochimBiophys Sin (Shanghai). 2009 Mar;41(3):198-205.

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[1] Miettinen HM. Regulation of human formyl peptide receptor 1 synthesis: role of single nucleotide polymorphisms, transcription factors, and inflammatory mediators. PLoS One. 2011;6(12):e28712.

[2] Zhang C, et al. Downregulation of PU.1 leads to decreased expression of Dectin-1 in alveolar macrophages during Pneumocystis pneumonia. Infect Immun. 2010 Mar;78(3):1058-65.

[5] Tsai CW, et al. Carnosic acid induces the NAD(P)H: quinoneoxidoreductase 1 expression in rat clone 9 cells through the p38/nuclear factor erythroid-2 related factor 2 pathway. J Nutr. 2011 Dec;141(12):2119-25.

[6] Wang DJ, et al. Regulation of the let-7a-3 promoter by NF-κB. PLoS One. 7(2):e31240 (2012).

[7] Tran TA, et al. Lipopolysaccharide and tumor necrosis factor regulate Parkin expression via nuclear factor-kappa B. PLoS One. 6(8):e23660 (2011).

[8] Robbins D, et al. Imaging NF-κB signaling in mice for screening anticancer drugs. Methods Mol Biol. 716:169-77 (2011).

[9] Dvorák Z, et al. Differential effects of selected natural compounds with anti-inflammatory activity on the glucocorticoid receptor and NF-kappaB in HeLa cells. ChemBiol Interact. 159(2):117-28 (2006).

[10] Ozeki M, et al. Response of cyclin B1 to ionizing radiation: regulation by NF-kappaB and mitochondrial antioxidant enzyme MnSOD. Anticancer Res. 24(5A):2657-63 (2004).