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NF-κB luciferase reporter plasimd熒光素酶報(bào)告基因(報(bào)告基因質(zhì)粒)
NF-κB luciferase reporter plasimd熒光素酶報(bào)告基因(報(bào)告基因質(zhì)粒)

地:Genomeditech

貨物所在地:上海上海市

更新時(shí)間:2024/9/1 21:01:14

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初級會員·12年
聯(lián)人:
曹女士
話:
400-6111-883
機(jī):
后:
4006-111-883
真:
86-21-34615995
址:
上海市浦東新區(qū)天雄路166弄1號3樓
網(wǎng)址:
www.yeasen.com

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供應(yīng)簡介
REPOTMNF-κB報(bào)告基因是吉滿生物自主研發(fā)的用于檢測NFκB轉(zhuǎn)錄活性水平為目的的報(bào)告基因。NF-κB基因在炎癥反應(yīng)、免疫應(yīng)答和抑制細(xì)胞凋亡等方面有著重要的作用。


詳細(xì)介紹

 

REPOTMNF-κB報(bào)告基因主要應(yīng)用于NF-κB信號通路的研究和基于NF-κB信號通路的藥物研究以及癌細(xì)胞中NF-KB的調(diào)控機(jī)制的研究。
pGMNF-ΚB-Lu是吉滿生物改造后的哺乳動物真核表達(dá)載體,在其多克隆位點(diǎn)插入了多個NFκB結(jié)合位點(diǎn),可以高靈敏度地檢測NFκB的激活水平。同時(shí),對載體中預(yù)測出的其它轉(zhuǎn)錄因子以外的結(jié)合位點(diǎn)進(jìn)行了適當(dāng)?shù)耐蛔?,增加了質(zhì)粒的轉(zhuǎn)錄因子結(jié)合特異性。另外,由于質(zhì)粒體積減小,使得NFκB報(bào)告基因質(zhì)粒更易于轉(zhuǎn)染。
 
質(zhì)粒圖譜:
 
質(zhì)粒元件信息:

NF-κB response element (NF-κB)
32-71
Minimal TA promoter (pTA)
100-122
Luciferase reporter gene
154-1816
SV40 late poly(A) signal
1851-2072
SV40 early promoter
2120-2538
Synthetic neomycin phosphotransferase(Neor) coding region
2563-3357
Synthetic poly(A) signal
3382-3430
Synthetic Beta-lactamase(Ampr) coding region
4545-5405
Synthetic poly(A) signal/transcriptional pause site
5510-5663

包裝清單:

產(chǎn)品編號
產(chǎn)品名稱
包裝
GM-021001
REPOTMNF-κB報(bào)告基因
1μg
-
說明書
-

 
使用說明:
pGMNF-Κb-Lu可以采用常規(guī)轉(zhuǎn)染方法轉(zhuǎn)染哺乳動物細(xì)胞。用熒光素酶檢測試劑盒或雙熒光素酶檢測試劑盒進(jìn)行檢測。
TNF-α、IL-1βLPS等是常見的NF-κB激活劑,可作為NF-Κb報(bào)告基因的陽性對照。
 
注意事項(xiàng):
本質(zhì)粒未經(jīng)吉滿生物允許不得用于任何商業(yè)用途,也不得移交給訂貨人實(shí)驗(yàn)室以外的任何人或單位。
為了您的健康,實(shí)驗(yàn)操作時(shí)請穿實(shí)驗(yàn)服和帶一次性手套。
 
保存條件:
-20保存。
 
參考文獻(xiàn):
[1] Chang CT, Lin H, Ho TY, Li CC, Lo HY, Wu SL, Huang YF, Liang JA, Hsiang CY.
Comprehensive assessment of host responses to ionizing radiation by nuclear factor-κB bioluminescence imaging-guided transcriptomic analysis.
PLoS One. 2011;6(8):e23682. doi: 10.1371/journal.pone.0023682. Epub 2011 Aug 24.
[2] Xu J, Ding WF, Shao KK, Wang XD, Wang GH, Li HQ, Wang HM.
Transcription of promoter from the human APRIL gene regulated by Sp1 and NF-kB.
Neoplasma. 2012;59(3):341-7. doi: 10.4149/neo_2012_044.
[3] Barham W, Sherrill T, Connelly L, Blackwell TS, Yull FE.
Intraductal injection of LPS as a mouse model of mastitis: signaling visualized via an NF-κB reporter transgenic.
J Vis Exp. 2012 Sep 4;(67):e4030. doi: 10.3791/4030.
[4] Salerno KM, Jing X, Diges CM, Davis BM, Albers KM.
TRAF family member-associated NF-kappa B activator (TANK) expression increases in injured sensory neurons and is transcriptionally regulated by Sox11.
neuroscience.2012.11.034.
[5] Nagasaki H, Yoshimura T, Aoki N.
Real-time monitoring of inflammation status in 3T3-L1 adipocytes possessing a secretory Gaussia luciferase gene under the control of nuclear factor-kappa B response element.
Biochem Biophys Res Commun. 2012 Apr 13;420(3):623-7. doi: 10.1016/j.bbrc.2012.03.049. Epub 2012 Mar 17.
[6] Wang DJ, Legesse-Miller A, Johnson EL, Coller HA.
Regulation of the let-7a-3 promoter by NF-κB.
PLoS One. 2012;7(2):e31240. doi: 10.1371/journal.pone.0031240. Epub 2012 Feb 13.
[7] Tran TA, Nguyen AD, Chang J, Goldberg MS, Lee JK, Tansey MG.
Lipopolysaccharide and tumor necrosis factor regulate Parkin expression via nuclear factor-kappa B.
PLoS One. 2011;6(8):e23660. doi: 10.1371/journal.pone.0023660. Epub 2011 Aug 17.
[8] Robbins D, Zhao Y.
Imaging NF-κB signaling in mice for screening anticancer drugs.
Methods Mol Biol. 2011;716:169-77. doi: 10.1007/978-1-61779-012-6_10.
[9] Dvorák Z, Vrzal R, Maurel P, Ulrichová J.
Differential effects of selected natural compounds with anti-inflammatory activity on the glucocorticoid receptor and NF-kappaB in HeLa cells.
Chem Biol Interact. 2006 Feb 1;159(2):117-28. Epub 2005 Nov 10
[10] Ozeki M, Tamae D, Hou DX, Wang T, Lebon T, Spitz DR, Li JJ.
Response of cyclin B1 to ionizing radiation: regulation by NF-kappaB and mitochondrial antioxidant enzyme MnSOD.
Anticancer Res. 2004 Sep-Oct;24(5A):2657-63.
 
產(chǎn)品價(jià)格:

貨號
名稱
產(chǎn)地
規(guī)格
報(bào)價(jià)/
GM021001
NF-κB luciferase reporter plasimd
熒光素酶報(bào)告基因(報(bào)告基因質(zhì)粒)
Genomeditech
1μg
800

 

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