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胰酶
參考價(jià) | 面議 |
- 型號(hào)
- 品牌 Biological Industries
- 廠商性質(zhì) 代理商
- 所在地 重慶市
更新時(shí)間:2016-12-20 09:20:08瀏覽次數(shù):3483
聯(lián)系我們時(shí)請(qǐng)說(shuō)明是化工儀器網(wǎng)上看到的信息,謝謝!
現(xiàn)貨供應(yīng),歡迎訂購(gòu): :
胰酶 產(chǎn)品信息:
Crystalline Trypsin Solution,貨號(hào) : 03-047-1A/B,胰酶
Cell Dissociation Solution(Non-Enzymatic),貨號(hào): 03-071-1B,(71號(hào))
71號(hào)細(xì)胞分離試劑是BI公司*開(kāi)發(fā)的不含任何酵素、蛋白水解酶及動(dòng)物組分的一種試劑。運(yùn)用離子螯合技術(shù)使貼壁細(xì)胞溫和的脫 落,zui大程度地保留完整的細(xì)胞表面蛋白及其生物功能,長(zhǎng)時(shí)間使用也無(wú)需擔(dān)心細(xì)胞受損??蛇m用于血清及不含血清培養(yǎng)細(xì)胞,貼壁更緊密的細(xì)胞可選用木瓜蛋白酶 或結(jié)晶。
Papain Dissociation Solution,貨號(hào) : 03-072-1B,
水晶對(duì)細(xì)胞損傷較小、細(xì)胞存活率高,較長(zhǎng)時(shí)間的使用也無(wú)需擔(dān)心。
木瓜蛋白酶與相比有更廣泛的特異性,更廣泛的降解蛋白物質(zhì),效率更高,對(duì)細(xì)胞、組織造成的損害更小,極少負(fù)面的影響,同時(shí)避免了動(dòng)物成分的污染。
的使用方法:
1. 使用不含鈣和鎂的PBS溶液沖洗細(xì)胞
2. 每一個(gè)T-25培養(yǎng)瓶中加1ml的木瓜蛋白酶分離溶液
3. 15-30°C孵育,當(dāng)細(xì)胞*分離,快速將細(xì)胞懸浮在5-10ml生長(zhǎng)培養(yǎng)基中。
4. 離心細(xì)胞,去掉上清
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重慶市華雅干細(xì)胞技術(shù)有限公司
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