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Anti-ATP5O 4C11C10D12 antibody (ab110276)抗ATP5O 4C
參考價(jià) | 面議 |
- 型號(hào) ab110276
- 品牌
- 廠商性質(zhì) 代理商
- 產(chǎn)品資料 查看pdf文檔
- 所在地 重慶市
更新時(shí)間:2017-09-25 10:12:45瀏覽次數(shù):2063
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貨號(hào):ab110276
廠家:Abcam公司
規(guī)格:100ug
保存溫度:-20℃ 和 2~8℃
貨期:現(xiàn)貨2~5天,期貨2-3周
Anti-ATP5O [4C11C10D12] antibody (ab110276)抗ATP5O [4C11C10D12]抗體產(chǎn)品信息:
- Product nameAnti-ATP5O [4C11C10D12] antibodySee all ATP5O primary antibodies ...
- Description
Mouse monoclonal [4C11C10D12] to ATP5O
- Tested applicationsICC/IF, WB, Flow Cyt, IHC-P more details
- Species reactivity
Reacts with: Cow, Human, African Green Monkey
- Immunogen
Recombinant mature Human ATP5O.
- Positive control
- Isolated mitochondria from Human heart and Bovine heart, Cultured Human embryonic lung-derived fibroblasts (strain MRC5), Human colon tissue, HL-60 cells
Properties
- FormLiquid
- Storage instructionsStore at +4°C. Do not freeze.
- Storage bufferPreservative: 0.02% Sodium azide
Constituent: HBS Concentration 100 µg at 1 mg/ml
- PurityIgG fraction
- Purification notesNear homogeneity as judged by SDS-PAGE. ab110276 was produced in vitro using hybridomas grown in serum-free medium, and then purified by biochemical fractionation.
- Clonality Monoclonal
- Clone number4C11C10D12
- IsotypeIgG1
- Light chain typekappa
- Research Areas
- Tags & Cell Markers
- Subcellular Markers
- Organelles
- Mitochondria
- Signal Transduction
- Metabolism
- Mitochondrial
- Metabolism
- Pathways and Processes
- Mitochondrial Metabolism
- Mitochondrial markers
- Metabolism
- Pathways and Processes
- Mitochondrial Metabolism
- Oxidative phosphorylation
- Complex V
- Metabolism
- Types of disease
- Cancer
Applications
Our Abpromise guarantee covers the use of ab110276 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Notes |
---|---|
ICC/IF | ICC/IF: Use a concentration of 0.5 - 1 µg/ml. heat-induced antigen-retrieval improves signal |
WB | WB: Use a concentration of 0.5 µg/ml. Predicted molecular weight: 23 kDa. |
Flow Cyt | Flow Cyt: Use a concentration of 1 µg/ml. |
IHC-P | IHC-P: 1/500. |
Target
- FunctionMitochondrial membrane ATP synthase (F(1)F(0) ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F(1) - containing the extramembraneous catalytic core and F(0) - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Part of the complex F(0) domain and the peripheric stalk, which acts as a stator to hold the catalytic alpha(3)beta(3) subcomplex and subunit a/ATP6 static relative to the rotary elements.
- Sequence similaritiesBelongs to the ATPase delta chain family.
- Cellular localizationMitochondrion. Mitochondrion inner membrane.
- Information by UniProt
Database links
- Entrez Gene: ATP5O Cow
- Entrez Gene: 539 Human
- Omim: 600828 Human
- SwissProt: P13621 Cow
- SwissProt: P48047 Human
- Unigene: 49172 Cow
- Unigene: 409140 Human
Alternative names
- ATP synthase O subunit mitochondrial precursor antibody
- ATP synthase subunit O antibody
- ATP synthase, H+ transporting, mitochondrial F1 complex, O subunit antibody
Anti-ATP5O [4C11C10D12] antibody images
Western blot - ATP5O antibody [4C11C10D12] (ab110276)
All lanes : Anti-ATP5O [4C11C10D12] antibody (ab110276) at 0.5 µg/ml
Lane 1 : Human heart mitochondria at 5 µg
Lane 2 : Bovine heart mitochondria at 1 µg
Predicted band size : 23 kDaImmunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - ATP5O antibody [4C11C10D12] (ab110276)
Human colon tissue fixed with 4% PFA and antibody detection with ab110276 using the ABC system. ab110276 diluted 1/500 and incubated for 1 hour. Sections were incubated in peroxidase-conjugated rabbit anti-mouse immunoglobulins (diluted 1/100 in 4% BSA in PBST) for 1 hour at room temp.
Immunocytochemistry/ Immunofluorescence - ATP5O antibody [4C11C10D12] (ab110276)
Mitochondrial localization of ATP5O. Cultured Human embryonic lung-derived fibroblasts (strain MRC5) were fixed, treated for heat-induced antigen retrieval, permeabilized and then labeled with ab110276 (0.5 µg/ml) followed by an AlexaFluor® 488-conjugated-goat-anti-mouse IgG1 isotype specific secondary antibody (2 µg/ml).
Flow Cytometry - ATP5O antibody [4C11C10D12] (ab110276)
HL-60 cells were stained with 1 µg/mL ab110276 (blue) or an equal amount of an isotype control antibody (red) and analyzed by flow cytometry.
Western blot - Anti-ATP5O antibody [4C11C10D12] (ab110276)
All lanes : Anti-ATP5O [4C11C10D12] antibody (ab110276) at 1 µg/ml
Lane 1 : HDFn (human) cell lysates at 20 µg
Lane 2 : COS7 (monkey) cell lysates at 20 µg
Lane 3 : H4IIE (rat) cell lysates at 20 µg
Lane 4 : MEF (mouse) cell lysates at 20 µg
Lane 5 : bovine heart mitochondria lysates at 5 µg
Predicted band size : 23 kDaSecondary antibody: Goat-Anti-Mouse-IR800 1:4000
公司介紹:
Abcam位于英國的劍橋科學(xué)園,成立于1998年,專門生產(chǎn)和分銷研究型抗體。主要?jiǎng)?chuàng)立人Jonathan Milner和實(shí)驗(yàn)室同事構(gòu)思成立一間了解科研人員需要的網(wǎng)上抗體公司,理念是Abcam銷售質(zhì)量高的抗體,并提供zui全面、誠實(shí)、時(shí)新的說明書,快速的送貨服務(wù),及高效率的顧客和服務(wù)。我們的在線目錄(www.hystemcell.cn) 已有差不多100,000種抗體和試劑,并不斷添加,供應(yīng)予百多個(gè)國家。Abcam于2005年11月在倫敦證券交易所上市,在美國馬薩諸塞州、日本東京及香港均設(shè)有分公司。
Abcam 一直致力加強(qiáng)產(chǎn)品線,為使研究員更容易找到蛋白質(zhì)研究試劑產(chǎn)品,在2011并購了美國的MitoSciences公司,加強(qiáng)了免疫分析方面的產(chǎn)品供應(yīng);同年也并購了英國的Ascent Scientific 公司,開展了生化試劑的供應(yīng)。在2012年,Abcam并購了美國的Epitomics 公司,成為一家有的RabMAbs® 供應(yīng)商。
Abcam 的 目標(biāo)是給世界上的抗體建立zui大的在線目錄,為各地科學(xué)家提供*產(chǎn)品,成為各國科學(xué)界的重要伙伴。我們?yōu)樗挟a(chǎn)品提供來使客戶獲得預(yù)期的結(jié) 果。為提供高質(zhì)量的抗體來指向盡可能多的靶蛋白,我們盡所能在盡可能多的應(yīng)用和物種中檢測(cè)每種抗體。我們相信誠信才是上策,在我們的上會(huì)發(fā)布有關(guān)我們 每一種產(chǎn)品的盡可能多的信息。
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