Origen二甲基亞砜DMSO及DMSO-DEX40

Origen 二甲基亞砜DMSO及DMSO-DEX40供應(yīng)

美國(guó)Origen

    DMSO 屬于非質(zhì)子極性溶劑，常用于化學(xué)反應(yīng)、 PCR反應(yīng)以及在細(xì)胞、組織和器官的保存中用作玻璃化低溫冷凍防護(hù)劑。 DMSO用于細(xì)胞冷凍培養(yǎng)基內(nèi)，可以保護(hù)細(xì)胞免受冰晶引起的機(jī)械性損傷。它也可以用于主培養(yǎng)、亞培養(yǎng)、重組異倍體、雜交瘤等系列細(xì)胞株，胚胎干細(xì)胞（ ESC）和造血干細(xì)胞的冷凍保藏。另外常常與 BSA或FBS混合使用。

用    途：

（1） DMSO廣泛應(yīng)用在人、動(dòng)物細(xì)胞株及細(xì)菌噬菌體λ的低溫防護(hù)中，制備 DMSO溶液用來(lái)冷凍細(xì)胞的步驟如下：

A.配制冷凍培養(yǎng)基：含有細(xì)胞培養(yǎng)用培養(yǎng)基、 10-20% 血清和 5-10% DMSO；

B.用胰酶或其他合適的方法消化貼壁細(xì)胞；（細(xì)胞處于對(duì)數(shù)增長(zhǎng)期）

C.低速離心（4℃，250×g, 10min）收集細(xì)胞，吸去培養(yǎng)基；

D.用冷凍培養(yǎng)基懸浮細(xì)胞，使細(xì)胞密度為 10⁶ -10⁷ cells/ml；

E.平均分配細(xì)胞，裝入凍存管內(nèi)；

F.依照標(biāo)準(zhǔn)凍存程序冷凍細(xì)胞，凍存在 -70℃或更低的溫度內(nèi)；

（2）細(xì)胞融合時(shí)，40-50% PEG 溶液內(nèi)加入10% DMSO；

（3）有文獻(xiàn)報(bào)道 DMSO作為緩沖液的成分，用于以下的實(shí)驗(yàn)：Poly（A+）RNA 的純化， E. coli感受態(tài)細(xì)胞轉(zhuǎn)化、 PCR反應(yīng)、 cDNA文庫(kù)的擴(kuò)增、 DNA測(cè)序、 DEAE-葡聚糖介導(dǎo)的細(xì)胞轉(zhuǎn)染、聚凝胺介導(dǎo)的 DNA轉(zhuǎn)染；

（4）有相應(yīng)的程序描述到用 DMSO回收膜過(guò)濾后的 DNA，用于接下來(lái)的 PCR擴(kuò)增反應(yīng)；

（5）用于 DNA測(cè)序的毛細(xì)管電泳技術(shù)將 5% DMSO (v/w)混入 2 M尿素中，如果需要可以調(diào)整 DMSO濃度到100%。

（6）有研究報(bào)道不同濃度的 DMSO與寡核苷酸的熔點(diǎn)溫度關(guān)系；

（7）有研究描述利用 DMSO提高雜交瘤細(xì)胞中單克隆抗體的產(chǎn)量； 調(diào)查發(fā)現(xiàn)低溫冷凍前用含有 DMSO的培養(yǎng)基高溫培養(yǎng)雜交瘤細(xì)胞。

（8）文獻(xiàn)報(bào)道利用 DMSO修飾蛋白中的磷酸絲氨酸和磷酸蘇氨酸殘基用作磷酸化狀態(tài)的質(zhì)譜分析（ MS）；

（9）文獻(xiàn)報(bào)道水和 DMSO中的亮脯利特（Leuprolide）降解作用；

互溶性：

? *互溶： LDPE, HDPE，聚丙烯 , PPCO, 聚甲基戊烯，尼龍， teflon FEP

? 中度互溶：聚苯乙烯， ECTFE/ETFE

? 不 互 溶：聚砜 ,， flexible and rigid PVC tubing，聚碳酸脂

注意事項(xiàng)：

    僅用于實(shí)驗(yàn)室，不可用于藥品、家居和其他用處 。

使用說(shuō)明：

    DMSO 易于水混合（ 1 ml DMSO + 1 ml H2O）形成清澈、無(wú)色的溶液。易吸潮，免暴露在潮濕環(huán)境。

儲(chǔ)存 /穩(wěn)定性：

DMSO 容易過(guò)冷并且室溫下緩慢融化，收到的產(chǎn)品應(yīng)為固體并非液體狀態(tài)。固化的 DMSO放在室溫下可以再次液化，對(duì)其本身無(wú)損害。屬于熱穩(wěn)定化合物，在高達(dá) 100℃的酸、堿及中性溶液中保持穩(wěn)定。溫度接近其沸點(diǎn)（189℃），在堿性和中性溶液中仍保持穩(wěn)定。 150℃加熱 24h，其純度損失小于0.1%。

要制備濾膜除菌的 DMSO溶液，推薦使用 teflon或 nylon膜，盡量不用醋酸纖維素膜。

產(chǎn)品基本信息：

1、CryoPur DMSO

OriGen manufactures sterile filtered CryoPurTM 100% DMSO and CryoPur-DTM DMSO/Dextran.

The solutions are packaged in syringes or vials. Both packages are shatter resistant. All syringes are shipped in a tray to prevent any damage while in transit, and are fitted with a stopcock, shipped with the actuator blocked for safety.

Our DMSO products are CE marked and certified USP and Pharmacopeia Europa Standards.

VIALS

CryoPurTM: 100% DMSO

Stock Number  Product Description  Stock Item    European Reference Number

CP-05    100% DMSO, 5ml Fill Volum              V10005

CP-10        100% DMSO, 10ml Fill Volume    X       V10010

CP-50        100% DMSO, 50ml Fill Volume         X     V10050

CP-70        100% DMSO, 70ml Fill Volume         X     V10070

CP-100     100% DMSO, 100ml Fill Volume       X     V10100

CryoPur-DTM: 55% w/v DMSO, Buffered with 5% w/v Dextran-40

(*CryoPur-D is a mixture of pure DMSO, Dextran-40 and Water for Injection, with the resulting solution being 55% w/v DMSO, 5% Dextran-40, and the remainder water for injection)

Stock Number  Product Description         Stock Item        European Reference Number

CD-05     DMSO/Dextran/remainder water, 5ml fill volume

CD-07       DMSO/Dextran/remainder water, 7.2ml fill volume       X       V504007

CD-35       DMSO/Dextran/remainder water, 35ml fill volume         X   V504035

CD-50       DMSO/Dextran/remainder water, 50ml fill volume      X     V504050

CD-70       DMSO/Dextran/remainder water, 70ml fill volume              V504070

CD-100     DMSO/Dextran/remainder water, 100ml fill volume    X     V5040100

CryoPur-STM: 55% w/v DMSO, Buffered with 5% w/v Dextran-40

(*CryoPur-S is a mixture of pure DMSO, Dextran-40 and remainder 0.72% Saline, with the resulting solution being 55% w/v DMSO, 5% Dextran-40, and the remainder saline.)

Stock Number  Product Description         Stock Item        European Reference Number

CDS-50B     DMSO/Dextran/saline, 50ml fill volume            V204850-S7

CDS-100B            DMSO/Dextran/saline, 100ml fill volume         V2048100-S7

SYRINGES

CryoPurTM: 100% DMSO

Stock Number  Product Description         Stock Item        European Reference Number

SP-10   100% DMSO, 10ml in 20ml syringe, stopcock and SCD tubing          X     S10010

SP-20    100% DMSO, 20ml in 20ml syringe, stopcock and SCD tubing       X      

SP-30        100% DMSO, 30ml in 60ml syringe, stopcock and SCD tubing        X     S10030

SP-50        100% DMSO, 50ml in 60ml syringe, stopcock and SCD tubing        X     S10050

SP-10-1     100% DMSO, 10ml in 20ml syringe, stopcock and cap           S10010SC

SP-50-1  100% DMSO, 50ml in 60ml syringe, stopcock and cap           S10050SC

CryoPur-DTM: 55% w/v DMSO, Buffered with 5% w/v Dextran-40

(*CryoPur-D is a mixture of pure DMSO, Dextran-40 and Water for Injection, with the resulting solution being 55% w/v DMSO, 5% Dextran-40, and the remainder water for injection)

Stock Number  Product Description         Stock Item        European Reference Number

SD-05   DMSO/Dextran/remainder water, 5ml in 20ml syringe, stopcock and SCD Tubing                 S504005

SD-06        DMSO/Dextran/remainder water, 6ml in 20ml syringe, stopcock and SCD Tubing                S504006

SD-07        DMSO/Dextran/remainder water, 7.2ml in 20ml syringe, stopcock and SCD Tubing     X     S504007

SD-10        DMSO/Dextran/remainder water, 10ml in 20ml syringe, stopcock and SCD Tubing      X       S504010

SD-12        DMSO/Dextran/remainder water, 12ml in 20ml syringe, stopcock and SCD Tubing              S504012

SD-10-1    DMSO/Dextran/remainder water, 10ml in 20ml syringe, stopcock and cap          S504010SC

CryoPur-STM: 55% w/v DMSO, Buffered with 5% w/v Dextran-40

(*CryoPur-S is a mixture of pure DMSO, Dextran-40 and remainder 0.9% Saline, with the resulting solution being 55% w/v DMSO, 5% Dextran-40, and the remainder saline.)

Stock Number  Product Description         Stock Item        European Reference Number

SDS-05A-B  DMSO/Dextran/saline, 5ml fill volume + 2ml air in 20ml syringe, stopcock and SCD Tubing      

SDS-15A   DMSO/Dextran/saline, 15ml in 20ml syringe, stopcock and SCD tubing       SDS-20A   DMSO/Dextran/saline, 20ml in 20ml syringe, stopcock and SCD tubing      

CryoPurTM: 80% DMSO, remainder water for injection

Stock Number  Product Description         Stock Item        European Reference Number

SPW-10C  DMSO, remainder water, 10ml in 20ml syringe, stopcock and SCD tubing       SPW-30C  DMSO, remainder water, 30ml in 60ml syringe, stopcock and SCD tubing       SPW-50C  DMSO, remainder water, 50ml in 60ml syringe, stopcock and SCD tubing            

Storage

100% DMSO: +20 to 30°C (freezes/solidifies at 18°C, DMSO/Dextran: +2 to 8°C

Packaging

10ml vials: 12 to a case, larger vials: 6 to a case, syringes sold individually

Custom Sizes

Custom packages and formulations are possible, please enquire. Minimum order volumes may apply.  8-12 week lead time on custom items.

USA: For research use only.

其他：

Cryostore:Freezing Bags  Freeze volumemin-max,10-30mL 凍存袋    24袋/箱        OriGen BioMedical    CS50

Cryostore:Freezing Bags  Freeze volumemin-max,30-70mL 凍存袋    24袋/箱        OriGen BioMedical    CS250

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